In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 4 ( 2022-4-14), p. e1010137-
Abstract:
Viral infections can alter host transcriptomes by manipulating host splicing machinery. Despite intensive transcriptomic studies on SARS-CoV-2, a systematic analysis of alternative splicing (AS) in severe COVID-19 patients remains largely elusive. Here we integrated proteomic and transcriptomic sequencing data to study AS changes in COVID-19 patients. We discovered that RNA splicing is among the major down-regulated proteomic signatures in COVID-19 patients. The transcriptome analysis showed that SARS-CoV-2 infection induces widespread dysregulation of transcript usage and expression, affecting blood coagulation, neutrophil activation, and cytokine production. Notably, CD74 and LRRFIP1 had increased skipping of an exon in COVID-19 patients that disrupts a functional domain, which correlated with reduced antiviral immunity. Furthermore, the dysregulation of transcripts was strongly correlated with clinical severity of COVID-19, and splice-variants may contribute to unexpected therapeutic activity. In summary, our data highlight that a better understanding of the AS landscape may aid in COVID-19 diagnosis and therapy.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010137
DOI:
10.1371/journal.pgen.1010137.g001
DOI:
10.1371/journal.pgen.1010137.g002
DOI:
10.1371/journal.pgen.1010137.g003
DOI:
10.1371/journal.pgen.1010137.g004
DOI:
10.1371/journal.pgen.1010137.g005
DOI:
10.1371/journal.pgen.1010137.g006
DOI:
10.1371/journal.pgen.1010137.s001
DOI:
10.1371/journal.pgen.1010137.s002
DOI:
10.1371/journal.pgen.1010137.s003
DOI:
10.1371/journal.pgen.1010137.s004
DOI:
10.1371/journal.pgen.1010137.s005
DOI:
10.1371/journal.pgen.1010137.s006
DOI:
10.1371/journal.pgen.1010137.s007
DOI:
10.1371/journal.pgen.1010137.s008
DOI:
10.1371/journal.pgen.1010137.s009
DOI:
10.1371/journal.pgen.1010137.s010
DOI:
10.1371/journal.pgen.1010137.s011
DOI:
10.1371/journal.pgen.1010137.s012
DOI:
10.1371/journal.pgen.1010137.s013
DOI:
10.1371/journal.pgen.1010137.s014
DOI:
10.1371/journal.pgen.1010137.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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