In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 6 ( 2021-6-16), p. e0253297-
Abstract:
Infectious salmon anaemia virus (ISAV) is the cause of an important waterborne disease of farmed Atlantic salmon. Detection of virus in water samples may constitute an alternative method to sacrificing fish for surveillance of fish populations for the presence of ISA-virus. We aimed to evaluate different membrane filters and buffers for concentration and recovery of ISAV in seawater, prior to molecular detection. One litre each of artificial and natural seawater was spiked with ISAV, followed by concentration with different filters and subsequent elution with different buffers. The negatively charged MF hydrophilic membrane filter, combined with NucliSENS ® lysis buffer, presented the highest ISAV recovery percentages with 12.5 ± 1.3% by RT-qPCR and 31.7 ± 10.7% by RT-ddPCR. For the positively charged 1 MDS Zeta Plus ® Virosorb ® membrane filter, combined with NucliSENS ® lysis buffer, the ISAV recovery percentages were 3.4 ± 0.1% by RT-qPCR and 10.8 ± 14.2% by RT-ddPCR. The limits of quantification (LOQ) were estimated to be 2.2 x 10 3 ISAV copies/L of natural seawater for both RT-qPCR and RT-ddPCR. The ISAV concentration method was more efficient in natural seawater.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0253297
DOI:
10.1371/journal.pone.0253297.g001
DOI:
10.1371/journal.pone.0253297.g002
DOI:
10.1371/journal.pone.0253297.g003
DOI:
10.1371/journal.pone.0253297.g004
DOI:
10.1371/journal.pone.0253297.t001
DOI:
10.1371/journal.pone.0253297.t002
DOI:
10.1371/journal.pone.0253297.t003
DOI:
10.1371/journal.pone.0253297.t004
DOI:
10.1371/journal.pone.0253297.s001
DOI:
10.1371/journal.pone.0253297.s002
DOI:
10.1371/journal.pone.0253297.s003
DOI:
10.1371/journal.pone.0253297.s004
DOI:
10.1371/journal.pone.0253297.s005
DOI:
10.1371/journal.pone.0253297.r001
DOI:
10.1371/journal.pone.0253297.r002
DOI:
10.1371/journal.pone.0253297.r003
DOI:
10.1371/journal.pone.0253297.r004
DOI:
10.1371/journal.pone.0253297.r005
DOI:
10.1371/journal.pone.0253297.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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