In:
Biochemical Society Transactions, Portland Press Ltd., Vol. 42, No. 6 ( 2014-12-01), p. 1498-1505
Abstract:
Breast cancer heterogeneity demands that prognostic models must be biologically driven and recent clinical evidence indicates that future prognostic signatures need evaluation in the context of early compared with late metastatic risk prediction. In pre-clinical studies, we and others have shown that various protein–protein interactions, pertaining to the actin microfilament-associated proteins, ezrin and cofilin, mediate breast cancer cell migration, a prerequisite for cancer metastasis. Moreover, as a direct substrate for protein kinase Cα, ezrin has been shown to be a determinant of cancer metastasis for a variety of tumour types, besides breast cancer; and has been described as a pivotal regulator of metastasis by linking the plasma membrane to the actin cytoskeleton. In the present article, we demonstrate that our tissue imaging-derived parameters that pertain to or are a consequence of the PKC–ezrin interaction can be used for breast cancer prognostication, with inter-cohort reproducibility. The application of fluorescence lifetime imaging microscopy (FLIM) in formalin-fixed paraffin-embedded patient samples to probe protein proximity within the typically & lt;10 nm range to address the oncological challenge of tumour heterogeneity, is discussed.
Type of Medium:
Online Resource
ISSN:
0300-5127
,
1470-8752
Language:
English
Publisher:
Portland Press Ltd.
Publication Date:
2014
SSG:
12
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