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  • 1
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    TET2 mRNA Expression in Bone Marrow Mononuclear Cells of the Patients with Myelodysplastic Syndromes and it's Clinical Significances
    American Society of Hematology ; 2011
    In:  Blood Vol. 118, No. 21 ( 2011-11-18), p. 5036-5036
    Details
    In: Blood, American Society of Hematology, Vol. 118, No. 21 ( 2011-11-18), p. 5036-5036
    Abstract: Abstract 5036 Objective This study was aimed to investigate the expression of TET2 mRNA in the bone marrow mononuclear cells(BMMNC)of patients with myelodysplastic syndrome(MDS)and its clinical significance. Methods The mRNA expression of TET2 in bone marrow mononuclear cells(BMMNC) of 25 patients with MDS and 16 controls were detected by RT-PCR. Results The expression of TET2 mRNA in BMMNC was down-regulated in MDS (0.9509±0.3841)compared with that in controls(1.2515±0.3749)(P 〈 0.05), but was no significant difference of BMMNC expression of TET2 among RA, RCMD and RAEB. Patients with higher expression of TET2(≥0.9) presented significantly lower proportion of bone marrow blasts[(1.04±1.68)%] than that [(6.13±8.17)%] of those with lower expression ( 〈 0.9) of TET2 (P 〈 0.05). The expression of TET2 mRNA in BMMNC of MDS patients was inversely correlated with malignant clone burden (r=-0.398,P 〈 0.05) and IPSS (r=-0.480,P 〈 0.05). Conclusions The mRNA expression of TET2 in BMMNC of MDS patients decreased, which might useful as an important indicator for the evaluation of MDS clone burden. Disclosures: No relevant conflicts of interest to declare.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V118.21.5036.5036
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2011
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  • 2
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    Expression of Differentiation Antigens on Bone Marrow Myeloid Cells of the Patients with Myelodysplastic Syndromes and it's Clinical Significances
    American Society of Hematology ; 2010
    In:  Blood Vol. 116, No. 21 ( 2010-11-19), p. 4967-4967
    Details
    In: Blood, American Society of Hematology, Vol. 116, No. 21 ( 2010-11-19), p. 4967-4967
    Abstract: Abstract 4967 Objective To study the abnormal differentiation of bone marrow myeloid cells in myelodysplastic syndromes (MDS) and its correlation with the prognosis of MDS patients. Methods Quantitative assessment of CD11b, CD13, CD16 and HLA-DR expression on the membrane of bone marrow granulocytes, and CD71 and glycophorin A on erythroblasts of 12 MDS patients in low-risk, 22 in high-risk and 31 normal controls was conducted with flow cytometry. The correlation between the abnormality of these antigen expression and the prognosis of MDS cases were analyzed. Results The granulocytic differentiation was analyzed with the combinations of CD13/CD11b, CD13/CD16 and CD11b/CD16. The “right hook”, “sickle” and “retroflex 7” shape expressions were found in normal controls while there were various changes in MDS groups. The ratios of CD11b-/CD11b+(0.39±0.34)and CD16-/CD16+(1.33 ±0.77)of high-risk MDS group were significantly higher than those of control group (0.07±0.05 and 0.39 ±0.31 respectively) (P 〈 0.05). The MFI (mean fluorescence index) of SSC (side scatter) in the granulocyte gate of MDS groups was lower while their MFI of CD13 was higher. The mean percentages of CD11b-HLA-DR+ (3.88%±3.07%), CD11b- HLA-DR- (16.23%±15.59%), CD16-HLA-DR- (41.12%±24.53%), CD11b+CD16- (33.53%±17.26%) and CD13+CD16- (44.51%±21.99%) granulocytes of high-risk MDS group were significantly higher than those of low-risk and control groups (P 〈 0.05). The erythroid cell lineage differentiation was analyzed with CD71/glycophorin A combination. Double antigen positive expression was found in all controls, but asynchronous expression of CD71/glycophorin A was found in some MDS cases. The mean percentage of double antigen positive cells in CD45- and glycophorin A+ cell population was significantly lower in low-risk and high-risk MDS groups. The abnormal numbers and patterns of the antigen expression of MDS cases correlated directly with their IPSS (international prognostic scoring system) (r=0.690, P=0.000) and WPSS (WHO adapted prognostic scoring system) (r=0.651, P=0.000) scores. Conclusion There were abnormal expressions of differentiation antigens on bone marrow myeloid cells of MDS patients. And the severity of these abnormal expressions was correlated with their prognosis. The abnormal differentiation of myeloid cells is probably involved in the pathogenesis of MDS. So the examination of these antigenic expressions with flow cytometry might be helpful for diagnosis and prognosis of MDS. Disclosures: No relevant conflicts of interest to declare.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V116.21.4967.4967
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2010
    detail.hit.zdb_id: 1468538-3
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  • 3
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    Characteristics of patients with autoimmune haemolytic anaemia secondary to lymphoproliferative disorder: A single-centre retrospective analysis
    Springer Science and Business Media LLC ; 2019
    In:  Scientific Reports Vol. 9, No. 1 ( 2019-12-23)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2019-12-23)
    Abstract: Autoimmune haemolytic anaemia (AIHA) is a kind of autoimmune diseases characterized by autoantibodies which produced and secreted by abnormal activated B lymphocytes directed against red blood cells (RBC). Study reveals that about 50% AIHA mainly occurs secondary to lymphoproliferative disorders (LPD) and autoimmune diseases. In this study, we aim to explore the characteristics of patients with AIHA secondary to LPD. Fifteen patients with AIHA secondary to LPD (secondary group) and 60 with primary AIHA (primary group) were retrospectively included. Patients in the secondary group [(59.40 ± 4.74) y] were older than those in the primary group [(47.53 ± 2.30) y] ( p  = 0.024). Reticulocyte counts were lower for the secondary group [(134.55 ± 20.67) × 10 9 /L] than for the primary group [(193.88 ± 27.32) × 10 9 /L] ( p  = 0.09). Haptoglobin was higher in the secondary (0.75 ± 0.19) g/L than in the primary group (0.34 ± 0.05) g/L ( p  = 0.004). The ratio of CD3 + CD4 + /CD3 + CD8 + was higher in the secondary (1.81 ± 0.41) than in the primary (1.05 ± 0.12) group ( p  = 0.025). Duration of remission was shorter in the secondary [(23.52 ± 5.20) months] than in the primary [(40.87 ± 3.92) months] group ( p  = 0.013). Relapse rate was higher for the secondary (33.3%) than for the primary (8.3%) group ( p  = 0.003). Mortality rate was higher in the secondary (33.3%) than in the primary (8.3%) group ( p  = 0.003). Progression-free survival was shorter in the secondary than in the primary group ( p  = 0.021). In conclusion, patients with AIHA secondary to LPD showed higher age at diagnosis, shorter remission time, and higher recurrence and mortality rates than did those with primary AIHA.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-019-56162-y
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
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  • 4
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    Study on the Membrane Hemopoietic Cytokine Receptor Expression on CD34+ Bone Marrow Cells of the Patients with Myelodysplastic Syndromes
    American Society of Hematology ; 2010
    In:  Blood Vol. 116, No. 21 ( 2010-11-19), p. 2908-2908
    Details
    In: Blood, American Society of Hematology, Vol. 116, No. 21 ( 2010-11-19), p. 2908-2908
    Abstract: Abstract 2908 Objectives This study was to detect if there were abnormalities of membrane hemopoietic cytokine receptor expression on CD34+ bone marrow cells in MDS. Methods 34 newly diagnosed MDS(12 in low-risk and 22 in high-risk) cases and 32 normal controls were enrolled in this study. Their CD34+CD38+ and CD34+CD38- bone marrow cells and the expressions of stem cell factor receptor(SCF-R),erythropoietin receptor (EpoR), granulocyte colony-stimulating factor receptor (G-CSFR) and thrombopoietin receptor (TpoR) on those cells were measured by flow cytometry. Results The mean percentage of CD34+ BMMNCs of MDS cases in high risk[(2.94±4.79)%)] was significantly higher than that of control group[(0.95±1.06)%] (P 〈 0.05). The mean percentages of CD34+CD38+ cells were significantly lower in low risk and high risk groups[(86.98±6.83)% and (83.57±9.86)% respectively] than that in control group [(92.41±3.43)%] , thus the percentage of CD34+CD38- cells was significantly higher in either low-risk or high-risk group[(13.03±6.84)% and (16.42±9.85)% respectively]than that in control group[(7.59±3.43)%] (P 〈 0.05). In control group, the mean percentage of antigen expression of EpoR was significantly lower in CD34+CD38+ cells [(17.72±20.24) %] than that in CD34+CD38- cells [(64.65±21.02)%] (P 〈 0.01), The expressions of SCF-R,G-CSFR and TpoR on CD34+CD38- cells were not significantly different from these on CD34+CD38+ cells. The expression of EpoR on CD34+CD38+ cells of low-risk and high-risk MDS groups[(7.01±6.82)% and (7.16±9.45)% respectively] were significantly lower than that of control group[(17.72±20. 24) %] (P 〈 0.05), The expression of G-CSFR on CD34+CD38+ cells of low-risk and high-risk MDS groups[(22.65±12.14)% and (26.50±19.65)% respectively] were significantly lower than that of control group[(45.13±23.41)%](P 〈 0.01). The amount of EpoR on CD34+CD38-cells of low-risk and high-risk MDS groups[(40.18±20.38)% and (28.58±17.00)% respectively] were significantly lower than that of control group[(64.65±21.02)%] (P 〈 0.01), The expression of TpoR on CD34+CD38- cells of low-risk and high-risk MDS groups[(4.46±7.45)% and (3.23±4.55)% respectively] were significantly lower than that of control group[(15.33±14.95)%] (P 〈 0.01). The incidence of cytopenia of MDS cases with low expression rates of hemopoietic cytokine receptors on CD34+cells were higher than that of MDS with high expression rates of hemopoietic cytokine receptors. Conclusions There were abnormalities of differentiation and membrane hemopoietic cytokine receptors expression of CD34+ bone marrow cells in MDS, which were associated with MDS cytopenia and might be useful for MDS diagnosis. Disclosures: No relevant conflicts of interest to declare.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V116.21.2908.2908
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2010
    detail.hit.zdb_id: 1468538-3
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  • 5
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    Analysis of Related Factors of Malignant Clone Burden of Patients with Myelodysplastic Syndromes
    American Society of Hematology ; 2010
    In:  Blood Vol. 116, No. 21 ( 2010-11-19), p. 4979-4979
    Details
    In: Blood, American Society of Hematology, Vol. 116, No. 21 ( 2010-11-19), p. 4979-4979
    Abstract: Abstract 4979 Objective To investigate the related factors of the malignant clone burden of patients with Myelodysplastic syndromes (MDS), and explore the risk factors of cytogenetic evolution of MDS. Methods Seventy-three cases of MDS patients were enrolled in this study who received treatment in the hematological department of General Hospital of Tianjin Medical University from 2004 to 2009. Statistics methods such as t-test, Chi-square test and Logistic regression analysis were used to investigate the correlation between the malignant clone burden and its related factors such as bone marrow dysplasia, peripheral blood and iron metabolism indexes. Furthermore, the risk factors of cytogenetic evolution of MDS were also analyzed. Results Odd number-nucleus erythrocytes, double-nucleus granulocytes, hypolobated neutrophils were significantly correlated to high malignant clone burden (P 〈 0.05). PAS positive patients and patients with dysplasia in three myeloid lineages, blasts in peripheral blood exhibited significantly higher malignant clone burden (P 〈 0.05). Patients with abnormal karyotype presented significantly higher level of serum ferritin and lower level of unsaturated iron binding capacity (UIBC) than those with normal karyotype (P 〈 0.05). Odd number-nucleus erythrocytes, megaloblastic granulocytes and high myeloid differentiation index (DI) are risk factors indicating cytogenetic evolution of MDS patients. Conclusion The indexes of high malignant clone burden of MDS include: odd number-nucleus erythrocytes, double-nucleus granulocytes, hypolobated neutrophils, dysplasia in three myeloid lineages, blasts in peripheral blood, positive PAS, high level of serum ferritin and low level of UIBC. The risk factors of cytogenetic evolution of MDS include: odd number-nucleus erythrocytes, megaloblastic granulocytes and high myeloid DI. Disclosures: No relevant conflicts of interest to declare.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V116.21.4979.4979
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2010
    detail.hit.zdb_id: 1468538-3
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  • 6
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    Study on the Dendritic Cell Subsets and Their Relationship with the Expressions of T-Bet and GATA-3 in Peripheral Lymphocytes of Severe Aplastic Anemia Patients
    American Society of Hematology ; 2008
    In:  Blood Vol. 112, No. 11 ( 2008-11-16), p. 4901-4901
    Details
    In: Blood, American Society of Hematology, Vol. 112, No. 11 ( 2008-11-16), p. 4901-4901
    Abstract: Objective To investigate the effects of the dendritic cell (DC) subsets and the transcriptive factors, T-bet and GATA-3, on immune imbalance in the pathogenesis of acquired severe aplastic anemia (SAA). Methods The mDCs(HLA-DR+Lin−CD11c+) and pDCs(HLA-DR+Lin−CD123+) in peripheral blood mononuclear cells (PBMNC) were measured with flow cytometry (FCM), the expressions of T-bet mRNA and GATA-3 mRNA in PBMNC with semiquantitive RT-PCR and the plasma levels of IFNγ and IL-4 with ELISA in 29 SAA patients (21 untreated and 8 recovered) and 16 healthy controls. Results The percentages of mDCs in PBMNC were (0.44±0.24) % and (0.73±0.30) % in untreated and recovered SAA patients respectively, either of which was higher than that of controls (0.29±0.10%) (P & lt;0.05). The percentage of pDCs in the untreated cases was lower than that of the recovered or controls [(0.18±0.14) % vs. (0.28±0.20) % and (0.29±0.13) %] (P & lt;0.05). mDCs/pDCs ratios were 3.45±2.71 and 2.90±0.95 in untreated and recovered groups respectively, either of which was higher than that of controls (1.15±0.56) (P & lt;0.05). No statistical difference of mDCs/pDCs was found between untreated and recovered patients (P & lt;0.05). The relative mRNA expressions of transcriptive factor T-bet were 0.37±0.07, 0.20±0.07 and 0.17±0.05 in 3 groups, respectively. The expression of T-bet of untreated patients was higher than that of recovered patients or healthy controls (P & lt;0.05). There was no statistical difference of GATA-3 expression among 3 groups(P & gt;0.05). T-bet/GATA-3 ratio was 0.72±0.13 in untreated patients, which was higher than that of recovered patients (0.33±0.08) or controls (0.35±0.11). The plasma level of IFNγ in the untreated cases was (50.9±1.1)ng/L, which was higher than that of recovered [(49.7±0.9) ng/L] or controls[(49.7±0.7) ng/L] . There were significant positive correlation between T-bet and mDCs/pDCs (r=0.445, P & lt;0.01), as well as T-bet and IFNγ (r=0.402, P & lt;0.01). Conclusion Both mDCs/pDCs and T-bet/GATA-3 ratios in SAA were higher and positively correlated which might contribute to the cascade activation of T lymphocytes. Immunosuppressive therapy for SAA should be kept on until both ratios become normal.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V112.11.4901.4901
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2008
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  • 7
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    In Vitro Study of the Function of Dendritic Cells on Stimulating T Lymphocytes Proliferation in Severe Aplastic Anemia
    American Society of Hematology ; 2008
    In:  Blood Vol. 112, No. 11 ( 2008-11-16), p. 4919-4919
    Details
    In: Blood, American Society of Hematology, Vol. 112, No. 11 ( 2008-11-16), p. 4919-4919
    Abstract: Objective To investigate the function of dendritic cells (DCs) in severe aplastic anemia (SAA). Methods Fifteen untreated patients, 10 recovered patients with SAA and 12 normal controls were enrolled in this study. Their mDCs were induced from their bone marrow mononuclear cells with rhIL-4, rhGM-CSF and rhTNF in vitro. Then mDCs were co-cultured with allogenic lymphocytes (mixture lymphocyte reaction, MLR). The growth rate of lymphocyte was measured by MTT colorimetry and the concentrations of IL-12 and IFNγ in MLR supernatant were measured by ELISA. The correlation between the growth rate and the concentration of IL-12 or IFNγ was analyzed. Results When mDCs and lymphocytes were co-cultured at the ratio of 1:50, the growth rate of lymphocytes stimulated with the mDCs from untreated cases was (322.13±171.07)%, which was higher than those stimulated with the mDCs from recovered and controls [(180.90±79.12)%, (192.25±91.93)%] (P & lt;0.05). There was no statistical significance between the growth rates of lymphocytes stimulated with the mDCs from the recovered and controls (P & gt;0.05). The mDCs of 8 recovered cases and 4 controls were co-cultured with allogenic lymphocytes at the ratio of 1:100, 1:50, 1:20 and 1:10, but no statistical significance was found between two groups at each ratio (P & gt;0.05). Cross MLR showed only untreated cases versus normal had statistical significance (P & lt;0.05). The concentrations of IL-12 and IFNγ in MLR supernatant of untreated cases were higher than those of the recovered or controls (P & lt;0.05), but there was no statistical significance between the recovered and controls (P & gt;0.05). The concentration of IL-12 in MLR supernatant correlated positively with the growth rate of lymphocyte (r = 0.529,P=0.001), which was also positively correlated with the concentration of IFNγ (r = 0.381,P=0.024). Conclusion The function of mDCs to stimulate T lymphocytes proliferation by secretingt IL-12 in SAA was enhanced, which might play an important role in the immunopathogenesis of SAA.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V112.11.4919.4919
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2008
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  • 8
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    CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia
    Spandidos Publications ; 2014
    In:  Molecular Medicine Reports Vol. 10, No. 3 ( 2014-9), p. 1252-1258
    Details
    In: Molecular Medicine Reports, Spandidos Publications, Vol. 10, No. 3 ( 2014-9), p. 1252-1258
    Type of Medium: Online Resource
    ISSN: 1791-2997 , 1791-3004
    URL: Article
    DOI: 10.3892/mmr.2014.2344
    Language: English
    Publisher: Spandidos Publications
    Publication Date: 2014
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  • 9
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    Increased Frequency of Bone Marrow T Follicular Helper Cells in Patients with Immune-Related Pancytopenia
    Hindawi Limited ; 2013
    In:  Clinical and Developmental Immunology Vol. 2013 ( 2013), p. 1-10
    Details
    In: Clinical and Developmental Immunology, Hindawi Limited, Vol. 2013 ( 2013), p. 1-10
    Abstract: Immune-related pancytopenia (IRP) is one kind of bone marrow failure diseases which is related to autoantibodies. Autoantibodies have been detected on the membrane of various bone marrow (BM) hemopoietic cells by BM mononuclear-cell-Coombs test or flow cytometric analysis. There are autoantibodies in the BM supernatant of IRP patients, which can target several antigens on hematopoietic cells membranes by western blot. T follicular helper (Tfh) cells are the true helper cells for Ab responses, which represent one of the most numerous and important subsets of effector T cells. Dysregulation of Tfh cell function or expression of Tfh cell-associated molecules could contribute to the pathogenesis of autoimmune diseases. Currently, there are no studies regarding the role of Tfh cells in IRP patients. The percentages of Tfh cells, Tfh-related molecules ICOS, CD40L, IL-21, and Bcl-6 in BM were investigated in 90 patients with IRP, and 25 healthy controls. We observed that there exist increased quantity and hyperfunction of Tfh cells in IRP, and the results were correlated with patient characteristics. It was indicated that dysregulated Tfh cells might be involved in the pathogenesis of IRP and that inhibition of Tfh cells effector molecules might provide opportunities for new therapeutic approaches to IRP and even other human autoimmune diseases.
    Type of Medium: Online Resource
    ISSN: 1740-2522 , 1740-2530
    URL: Article
    DOI: 10.1155/2013/730450
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2013
    detail.hit.zdb_id: 2817541-4
    detail.hit.zdb_id: 2119272-8
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  • 10
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    Transforming growth factor 15 increased in severe aplastic anemia patients
    Informa UK Limited ; 2017
    In:  Hematology Vol. 22, No. 9 ( 2017-10-21), p. 548-553
    Details
    In: Hematology, Informa UK Limited, Vol. 22, No. 9 ( 2017-10-21), p. 548-553
    Type of Medium: Online Resource
    ISSN: 1607-8454
    URL: Article
    DOI: 10.1080/10245332.2017.1311462
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2017
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