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  • 1
    Online Resource
    Online Resource
    Extracellular Matrix Expression in Human Induced Pluripotent Stem Cell-Derived Optic Vesicles
    Science Repository OU ; 2021
    In:  International Journal of Regenerative Medicine
    Details
    In: International Journal of Regenerative Medicine, Science Repository OU
    Abstract: Background: Human tissue/organ development is a complex, highly orchestrated process, regulated in part by the surrounding extracellular matrix (ECM). Every complex tissue, including the retina, has a unique ECM configuration that plays a critical role in cellular differentiation, adhesion, migration, and maturation. Aim: To characterize ECM expression of human induced pluripotent stem cell-derived optic vesicles (iPSC-OVs). Methods: A 3- dimensional (3D) in vitro suspension culture system was used to direct differentiation of human induced pluripotent stem cells (iPSCs) into optic vesicles (OVs). Stepwise differentiation of iPSCs into retinal progenitor cells was confirmed by sequential expression of OTX2, SOX1, SIX6, LHX2, PAX6, and CHX10. Expression of ECM genes in iPSC-derived OVs was analyzed by RT2 ProfilerTM PCR Array, whereas immunofluorescence staining was performed to detect ECM proteins in the OVs. Results: A number of cell adhesion molecules (CAMs) previously reported to be abundantly expressed in iPSCs such as E-cadherin, Intercellular adhesio n molecule-1 (ICAM1), Integrin-α L, Integrin-α M, Integrin-α 6 were downregulated while neural and retina specific CAMs including neural cell adhesion molecule 1 (NCAM1), neural plakophilin-related armadillo repeat protein (NPRAP), Integrin-α 1 and Integrin-α 4 were upregulated. Several glycoproteins that have been reported to play key roles during retinogenesis, namely CD44, Tenascin C, Tenascin R, Neurocan, Neuroglycan C, Delta 2 Catenin, Vitronectin, and Reelin were also present. Conclusion: We have identified an array of ECM proteins that were expressed during retinogenesis. Further characterization of these proteins will lead to a better understanding of retinal development.
    Type of Medium: Online Resource
    ISSN: 2613-5914 , 2613-5914
    URL: Journal
    URL: Article
    DOI: 10.31487/j.RGM
    DOI: 10.31487/j.RGM.2021.02.01
    Language: Unknown
    Publisher: Science Repository OU
    Publication Date: 2021
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  • 2
    Online Resource
    Online Resource
    CLIC4 regulates late endosomal trafficking and matrix degradation activity of MMP14 at focal adhesions in RPE cells
    Springer Science and Business Media LLC ; 2019
    In:  Scientific Reports Vol. 9, No. 1 ( 2019-08-22)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2019-08-22)
    Abstract: Dysregulation in the extracellular matrix (ECM) microenvironment surrounding the retinal pigment epithelium (RPE) has been implicated in the etiology of proliferative vitreoretinopathy and age-related macular degeneration. The regulation of ECM remodeling by RPE cells is not well understood. We show that membrane-type matrix metalloproteinase 14 (MMP14) is central to ECM degradation at the focal adhesions in human ARPE19 cells. The matrix degradative activity, but not the assembly, of the focal adhesion is regulated by chloride intracellular channel 4 (CLIC4). CLIC4 is co-localized with MMP14 in the late endosome. CLIC4 regulates the proper sorting of MMP14 into the lumen of the late endosome and its proteolytic activation in lipid rafts. CLIC4 has the newly-identified “late domain” motif that binds to MMP14 and to Tsg101, a component of the endosomal sorting complex required for transport (ESCRT) complex. Unlike the late domain mutant CLIC4, wild-type CLIC4 can rescue the late endosomal sorting defect of MMP14. Finally, CLIC4 knockdown inhibits the apical secretion of MMP2 in polarized human RPE monolayers. These results, taken together, demonstrate that CLIC4 is a novel matrix microenvironment modulator and a novel regulator for late endosomal cargo sorting. Moreover, the late endosomal sorting of MMP14 actively regulates its surface activation in RPE cells.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-019-48438-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
    detail.hit.zdb_id: 2615211-3
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  • 3
    Online Resource
    Online Resource
    Profiling the microRNA Expression in Human iPS and iPS-derived Retinal Pigment Epithelium
    SAGE Publications ; 2014
    In:  Cancer Informatics Vol. 13s5 ( 2014-01), p. CIN.S14074-
    Details
    In: Cancer Informatics, SAGE Publications, Vol. 13s5 ( 2014-01), p. CIN.S14074-
    Abstract: The purpose of this study is to characterize the microRNA (miRNA) expression profiles of induced pluripotent stem (iPS) cells and retinal pigment epithelium (RPE) derived from induced pluripotent stem cells (iPS-RPE). MiRNAs have been demonstrated to play critical roles in both maintaining pluripotency and facilitating differentiation. Gene expression networks accountable for maintenance and induction of pluripotency are linked and share components with those networks implicated in oncogenesis. Therefore, we hypothesize that miRNA expression profiling will distinguish iPS cells from their iPS-RPE progeny. To identify and analyze differentially expressed miRNAs, RPE was derived from iPS using a spontaneous differentiation method. MiRNA microarray analysis identified 155 probes that were statistically differentially expressed between iPS and iPS-RPE cells. Up-regulated miRNAs including miR-181c and miR-129–5p may play a role in promoting differentiation, while down-regulated miRNAs such as miR-367, miR-18b, and miR-20b are implicated in cell proliferation. Subsequent miRNA-target and network analysis revealed that these miRNAs are involved in cellular development, cell cycle progression, cell death, and survival. A systematic interrogation of temporal and spatial expression of iPS-RPE miRNAs and their associated target mRNAs will provide new insights into the molecular mechanisms of carcinogenesis, eye differentiation and development.
    Type of Medium: Online Resource
    ISSN: 1176-9351 , 1176-9351
    URL: Article
    DOI: 10.4137/CIN.S14074
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2014
    detail.hit.zdb_id: 2202739-7
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  • 4
    Online Resource
    Online Resource
    High Levels of CXCL10 Are Produced by Intestinal Epithelial Cells in AIDS Patients with Active Cryptosporidiosis but Not after Reconstitution of Immunity
    American Society for Microbiology ; 2007
    In:  Infection and Immunity Vol. 75, No. 1 ( 2007-01), p. 481-487
    Details
    In: Infection and Immunity, American Society for Microbiology, Vol. 75, No. 1 ( 2007-01), p. 481-487
    Abstract: Chemokines play key roles in attracting immune cells to sites of infections. However, few data on chemokine expression in the gut during human infections are available. We examined expression of chemokines in intestinal tissues of AIDS patients during active Cryptosporidium infection and during resolution of such an infection. The chemokines and cytokines in cell lysates from jejunal biopsy tissues were assayed by a 22-multiplex bead immunoassay. CXCL10 (IP-10) and its receptor, CXCR3, in sections were studied by immunohistochemistry. In biopsies from AIDS patients with active cryptosporidiosis, four chemokines (CXCL10, CCL11 [eotaxin] , CCL5 [RANTES], and CCL2 [monocyte chemoattractant protein 1] ) and three cytokines (interleukin-1α [IL-1α], IL-10, and granulocyte colony-stimulating factor) were detected. The level of CXCL10 was significantly increased in AIDS patients with cryptosporidiosis compared to the level in AIDS patients without cryptosporidiosis or in normal volunteers (median in AIDS patients with cryptosporidiosis, 508 pg/mg protein, compared to 111 pg/mg and 72 pg/mg protein in AIDS patients without cryptosporidiosis and in normal volunteers, respectively [ P 〈 0.05 and P 〈 0.005, respectively, as determined by a Mann-Whitney test]). The level of CXCL10 correlated with the parasite burden (as measured by the number of Cryptosporidium oocysts in the stools) and also with the IL-1α concentration (Pearson correlation values, 0.961 [ P 〈 0.01] and 0.737 [ P 〈 0.05]). As determined by immunohistochemistry, CXCL10 localized to epithelial cells at the site of infection. Following effective antiparasite and antiretroviral therapy, Cryptosporidium infections resolved, and the levels of CXCL10 decreased to normal levels. We hypothesized that CXCL10 plays an important role in the resolution of cryptosporidiosis by attracting immune effector cells to the site of infection. By contrast, in AIDS patients lacking effector cells, CXCL10 may contribute to the immunopathogenesis by recruiting inflammatory cells.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    URL: Article
    DOI: 10.1128/IAI.01237-06
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2007
    detail.hit.zdb_id: 1483247-1
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  • 5
    Online Resource
    Online Resource
    Rapid and Transient Reduction in Circulating Thyroid Hormones Following Systemic Antigen Priming: Implications for Functional Collaboration between Dendritic Cells and Thyroid
    Elsevier BV ; 2001
    In:  Cellular Immunology Vol. 212, No. 2 ( 2001-09), p. 92-100
    Details
    In: Cellular Immunology, Elsevier BV, Vol. 212, No. 2 ( 2001-09), p. 92-100
    Type of Medium: Online Resource
    ISSN: 0008-8749
    URL: Article
    DOI: 10.1006/cimm.2001.1846
    RVK:
    XA 10000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2001
    detail.hit.zdb_id: 1462601-9
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  • 6
    Online Resource
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    Comparative evaluation of mesenchymal stromal cell growth and osteogenic differentiation on a shape memory polymer scaffold
    Wiley ; 2022
    In:  Journal of Biomedical Materials Research Part B: Applied Biomaterials Vol. 110, No. 9 ( 2022-09), p. 2063-2074
    Details
    In: Journal of Biomedical Materials Research Part B: Applied Biomaterials, Wiley, Vol. 110, No. 9 ( 2022-09), p. 2063-2074
    Abstract: Trauma‐induced, critical‐size bone defects pose a clinical challenge to heal. Albeit autografts are the standard‐of‐care, they are limited by their inability to be shaped to various defect geometries and often incur donor site complications. Herein, the combination of a “self‐fitting” shape memory polymer (SMP) scaffold and seeded mesenchymal stromal cells (MSCs) was investigated as an alternative. The porous SMP scaffold, prepared from poly(ε‐caprolactone) diacrylate (PCL‐DA) and coated with polydopamine, provided conformal shaping and cell adhesion. MSCs from five tissues, amniotic (AMSCs), chorionic tissue (CHSCs), umbilical cord (UCSCs), adipose (ADSCs), and bone marrow (BMSCs) were evaluated for viability, density, and osteogenic differentiation on the SMP scaffold. BMSCs exhibited the fastest increase in cell density by day 3, but after day 10, CHSCs, UCSCs, and ADSCs approached similar cell density. BMSCs also showed the greatest calcification among the cell types, followed closely by ADSCs, CHSCs and AMSCs. Alkaline phosphatase (ALP) activity peaked at day 7 for AMSCs, UCSCs, ADSCs and BMSCs, and at day 14 for CHSCs, which had the greatest overall ALP activity. Of all the cell types, only scaffolds cultured with ADSCs in osteogenic media had increased hardness and local modulus as compared to blank scaffolds after 21 days of cell culture and osteogenic differentiation. Overall, ADSCs performed most favorably on the SMP scaffold. The SMP scaffold was able to support key cellular behaviors of MSCs and could potentially be a viable, regenerative alternative to autograft.
    Type of Medium: Online Resource
    ISSN: 1552-4973 , 1552-4981
    URL: Issue
    URL: Article
    DOI: 10.1002/jbm.b.v110.9
    DOI: 10.1002/jbm.b.35061
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2130917-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Multiple Levels of Activation of Murine CD8+ Intraepithelial Lymphocytes Defined by OX40 (CD134) Expression: Effects on Cell-Mediated Cytotoxicity, IFN-γ, and IL-10 Regulation
    The American Association of Immunologists ; 2001
    In:  The Journal of Immunology Vol. 167, No. 12 ( 2001-12-15), p. 6717-6723
    Details
    In: The Journal of Immunology, The American Association of Immunologists, Vol. 167, No. 12 ( 2001-12-15), p. 6717-6723
    Abstract: The involvement of OX40 (CD134) in the activation of CD8+ intestinal intraepithelial lymphocytes (IELs) has been studied using freshly isolated IELs and in vitro CD3-stimulated IELs. Although freshly isolated CD8+ IELs exhibited properties of activated T cells (CD69 expression and ex vivo cytotoxicity), virtually all CD8+ IELs from normal mice were devoid of other activation-associated properties, including a lack of expression of OX40 and the ligand for OX40 (OX40L) and an absence of intracellular IFN-γ staining. However, OX40 and OX40L expression were rapidly up-regulated on CD8 IELs following CD3 stimulation, indicating that both markers on IELs reflect activation-dependent events. Unlike IELs, activated lymph node T cells did not express OX40L, thus indicating that OX40-OX40L communication in the intestinal epithelium is part of a novel CD8 network. Functionally, OX40 expression was exclusively associated with IELs with active intracellular IFN-γ synthesis and markedly enhanced cell-mediated cytotoxicity. However, OX40 costimulation during CD3-mediated activation significantly suppressed IL-10 synthesis by IELs, whereas blockade of OX40-OX40L by anti-OX40L mAb markedly increased IL-10 production. These findings indicate that: 1) resident CD69+OX40− IELs constitute a population of partially activated T cells poised for rapid delivery of effector activity, 2) OX40 and OX40L expression defines IELs that have undergone recent immune activation, 3) OX40+ IELs are significantly more efficient CTL than are OX40− IELs, and 4) the local OX40/OX40L system plays a critical role in regulating the magnitude of cytokine responses in the gut epithelium.
    Type of Medium: Online Resource
    ISSN: 0022-1767 , 1550-6606
    URL: Article
    DOI: 10.4049/jimmunol.167.12.6717
    RVK:
    XA 54100
    RVK:
    XA 10000
    Language: English
    Publisher: The American Association of Immunologists
    Publication Date: 2001
    detail.hit.zdb_id: 1475085-5
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  • 8
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    Online Resource
    Polarized Secretion of Matrix Metalloproteinases and Their Inhibitors by Retinal Pigment Epithelium Derived from Induced Pluripotent Stem Cells During Wound Healing
    Mary Ann Liebert Inc ; 2017
    In:  Journal of Ocular Pharmacology and Therapeutics Vol. 33, No. 3 ( 2017-04), p. 132-140
    Details
    In: Journal of Ocular Pharmacology and Therapeutics, Mary Ann Liebert Inc, Vol. 33, No. 3 ( 2017-04), p. 132-140
    Type of Medium: Online Resource
    ISSN: 1080-7683 , 1557-7732
    URL: Article
    DOI: 10.1089/jop.2016.0070
    Language: English
    Publisher: Mary Ann Liebert Inc
    Publication Date: 2017
    detail.hit.zdb_id: 2049089-6
    SSG: 15,3
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  • 9
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    Sealing of Corneal Lacerations Using Photoactivated Rose Bengal Dye and Amniotic Membrane
    Ovid Technologies (Wolters Kluwer Health) ; 2018
    In:  Cornea Vol. 37, No. 2 ( 2018-02), p. 211-217
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 37, No. 2 ( 2018-02), p. 211-217
    Abstract: Watertight closure of perforating corneoscleral lacerations is necessary to prevent epithelial ingrowth, infection, and potential loss of the eye. Complex lacerations can be difficult to treat, and repair with sutures alone is often inadequate. In this study, we evaluated a potentially sutureless technology for sealing complex corneal and scleral lacerations that bonds the amniotic membrane (AM) to the wound using only green light and rose bengal dye. Methods: The AM was impregnated with rose bengal and then sealed over lacerations using green light to bond the AM to the deepithelialized corneal surface. This process was compared with suture repair of 3 laceration configurations in New Zealand White rabbits in 3 arms of the study. A fourth study arm assessed the side effect profile including viability of cells in the iris, damage to the blood–retinal barrier, retinal photoreceptors, retinal pigment epithelium, and choriocapillaris in Dutch Belted rabbits. Results: Analyses of the first 3 arms revealed a clinically insignificant increase in polymorphonuclear inflammation. In the fourth arm, iris cells appeared unaffected and no evidence of breakdown of the blood–retinal barrier was detected. The retina from green light laser-treated eyes showed normal retinal pigment epithelium, intact outer segments, and normal outer nuclear layer thickness. Conclusions: The results of these studies established that a light-activated method to cross-link AM to the cornea can be used for sealing complex penetrating wounds in the cornea and sclera with minimal inflammation or secondary effects.
    Type of Medium: Online Resource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000001389
    RVK:
    XA 37258
    RVK:
    XA 10000
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2018
    detail.hit.zdb_id: 2045943-9
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  • 10
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    Immune Function of Thyroid Stimulating Hormone and Receptor
    Begell House ; 2001
    In:  Critical Reviews™ in Immunology Vol. 21, No. 4 ( 2001), p. 15-
    Details
    In: Critical Reviews™ in Immunology, Begell House, Vol. 21, No. 4 ( 2001), p. 15-
    Type of Medium: Online Resource
    ISSN: 2162-6472
    URL: Issue
    URL: Article
    DOI: 10.1615/CritRevImmunol.v21.i4
    DOI: 10.1615/CritRevImmunol.v21.i4.20
    Language: English
    Publisher: Begell House
    Publication Date: 2001
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