In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-20), p. e0254486-
Abstract:
The coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body’s immune response from natural COVID-19 illness or vaccines’ effects is urgently needed. In the present study, an attempt was made to integrate a newly designed spectrometer to the COVID-19 test strip procedure; this augmentation provides the quantitative capacity to a lateral flow immunoassay (LFIA). Optical interpretation of results by quantitative α index, rather than visual qualification, can be done quickly, in 5–10 minutes. The developed product was compared with several other serological IgM/IgG antibody reagents on the market by recruiting 111 participants suspected of having COVID-19 infection from March to May 2020 in a hospital. Taking RT-PCR as the diagnostic gold standard, the quantitative spectral LIFA platform could correctly detect all 12 COVID-19 patients. Concerning RT-PCR negative patients, all three antibody testing methods found positive cases. The optical-based platform exhibited the ability of early detection of immunoglobulins of RT-PCR negative patients. There was an apparent trend that elevation of IgM levels in the acute phase of infection; then IgG levels rose later. It exhibited the risk of a false-negative diagnosis of RT-PCR in COVID-19 testing. The significant detection ability of this new optical-based platform demonstrated clinical potential.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0254486
DOI:
10.1371/journal.pone.0254486.g001
DOI:
10.1371/journal.pone.0254486.g002
DOI:
10.1371/journal.pone.0254486.g003
DOI:
10.1371/journal.pone.0254486.g004
DOI:
10.1371/journal.pone.0254486.g005
DOI:
10.1371/journal.pone.0254486.g006
DOI:
10.1371/journal.pone.0254486.g007
DOI:
10.1371/journal.pone.0254486.t001
DOI:
10.1371/journal.pone.0254486.t002
DOI:
10.1371/journal.pone.0254486.t003
DOI:
10.1371/journal.pone.0254486.t004
DOI:
10.1371/journal.pone.0254486.s001
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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