In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_8 ( 2013-08-01), p. 2963-2969
Abstract:
Two Gram-negative, non-motile, rod-shaped strains, BQ4-1 T and NHI3-2, isolated respectively from the healthy and diseased part of Populus × euramericana canker bark, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of the two strains in the genus Acinetobacter , with genomic DNA G+C contents (42.5–43 mol%) within the range observed for this genus (38–47 mol%) and 9-octadecenoic acid (C 18 : 1 ω9 c , 39.87 %), hexadecanoic acid (C 16 : 0 , 11.26 %) and summed feature 3 (comprising C 16 : 1 ω7 c /C 16 : 1 ω6 c , 18.90 %) as major fatty acids. Phylogenetic analysis based on 16S rRNA, rpoB and gyrB gene sequences revealed that strains BQ4-1 T and NHI3 did not cluster with any species with validly published names, and formed a distinct cluster with 99–100 % bootstrap support on three phylogenetic trees within the genus Acinetobacter . Acid was not produced from d -glucose, and haemolysis was not observed on agar media supplemented with sheep erythrocytes. On the basis of phenotypic, genotypic and phylogenetic characteristics, the two strains are considered to represent a novel species of the genus Acinetobacter , for which the name Acinetobacter puyangensis sp. nov. is proposed. The type strain is BQ4-1 T ( = CFCC 10780 T = JCM 18011 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.047274-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
2056611-6
detail.hit.zdb_id:
2002336-4
SSG:
12
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