In:
Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 6, No. 3 ( 2009-12), p. 215-219
Abstract:
Single-nucleotide polymorphisms (SNPs) of exon 8 of the GHR gene were detected in Chinese Holstein cows by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The results showed that exon 8 of the GHR gene digested by Tas I could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and T were 0.6339 and 0.3661, respectively. The frequencies of genotypes AA, AT and TT were 0.459, 0.350 and 0.191, respectively. Sequencing showed one single nucleotide mutation T→A at 4962 bp of the gene in genotype TT when compared with genotype AA, and this mutation resulted in an amino acid change of phenylalanine (TTT)→tyrosine (TAT). The result of χ 2 testing indicated that the genotypic frequency of the GHR gene digested by Tas I did not fit with Hardy–Weinberg equilibrium in this population ( P 〈 0.05). The least-square mean of the fat percentage of this population was higher for genotype TT than for genotype AA ( P 〈 0.05).
Type of Medium:
Online Resource
ISSN:
1479-2362
,
1479-2370
DOI:
10.1017/S1479236209990283
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2009
detail.hit.zdb_id:
2188055-4
detail.hit.zdb_id:
2198867-5
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