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  • 1
    Online Resource
    Online Resource
    Design of optical fiber data link simulator
    Journal of Applied Optics ; 2015
    In:  Journal of Applied Optics Vol. 36, No. 3 ( 2015), p. 480-485
    Details
    In: Journal of Applied Optics, Journal of Applied Optics, Vol. 36, No. 3 ( 2015), p. 480-485
    Type of Medium: Online Resource
    ISSN: 1002-2082
    URL: Article
    DOI: 10.5768/JAO201536.0308001
    Language: English
    Publisher: Journal of Applied Optics
    Publication Date: 2015
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  • 2
    Online Resource
    Online Resource
    Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2
    Polish Botanical Society ; 2013
    In:  Acta Societatis Botanicorum Poloniae Vol. 82, No. 4 ( 2013), p. 283-288
    Details
    In: Acta Societatis Botanicorum Poloniae, Polish Botanical Society, Vol. 82, No. 4 ( 2013), p. 283-288
    Abstract: To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the 〈 em 〉 Schisandra chinensis 〈 /em 〉 at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the 〈 em 〉 Schisandra sphenanthera 〈 /em 〉 samples differed from that of 〈 em 〉 S. chinensis 〈 /em 〉 . Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both 〈 em 〉 S. chinensis 〈 /em 〉 and 〈 em 〉 S. sphenanthera 〈 /em 〉 showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for 〈 em 〉 S. chinensis 〈 /em 〉 , with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify 〈 em 〉 S. chinensis 〈 /em 〉 and 〈 em 〉 S. sphenanthera 〈 /em 〉 in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of 〈 em 〉 S. chinensis 〈 /em 〉 . Hence, the ITS2 is a powerful and efficient tool for species identification of 〈 em 〉 S. chinensis 〈 /em 〉 .
    Type of Medium: Online Resource
    ISSN: 2083-9480
    URL: Article
    DOI: 10.5586/asbp.2013.032
    Language: Unknown
    Publisher: Polish Botanical Society
    Publication Date: 2013
    detail.hit.zdb_id: 2574892-0
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  • 3
    Online Resource
    Online Resource
    Applying self-injection and dual-ring based fiber laser for wide tunability and stable single-frequency output
    Elsevier BV ; 2021
    In:  Optics & Laser Technology Vol. 137 ( 2021-05), p. 106804-
    Details
    In: Optics & Laser Technology, Elsevier BV, Vol. 137 ( 2021-05), p. 106804-
    Type of Medium: Online Resource
    ISSN: 0030-3992
    URL: Article
    DOI: 10.1016/j.optlastec.2020.106804
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2000654-8
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  • 4
    Online Resource
    Online Resource
    Generation and expression analysis of BAC humanized mice carrying HLA‐DP401 haplotype
    Wiley ; 2021
    In:  Animal Models and Experimental Medicine Vol. 4, No. 2 ( 2021-06), p. 116-128
    Details
    In: Animal Models and Experimental Medicine, Wiley, Vol. 4, No. 2 ( 2021-06), p. 116-128
    Abstract: Human leukocyte antigen (HLA)‐DP is much less studied than other HLA class II antigens, that is, HLA‐DR and HLA‐DQ, etc. However, the accumulating data have suggested the important roles of DP‐restricted responses in the context of cancer, allergy, and infectious disease. Lack of animal models expressing these genes as authentic cis ‐haplotypes blocks our understanding for the role of HLA‐DP haplotypes in immunity. Methods To explore the potential cis ‐acting control elements involved in the transcriptional regulation of the HLA‐DPA1/DPB1 gene, we performed the expression analysis using bacterial artificial chromosome (BAC)‐based transgenic humanized mice in the C57BL/6 background, which carried the entire HLA‐DP401 gene locus. We further developed a mouse model of Staphylococcus aureus pneumonia in HLA‐DP401 humanized transgenic mice, and performed the analysis on the expression pattern of HLA‐DP401 and immunological responses in the model. Results In this study, we screened and identified a BAC clone spanning the entire HLA‐DP gene locus. DNA from this clone was analyzed for integrity by pulsed‐field gel electrophoresis and then microinjected into fertilized mouse oocytes to produce transgenic founder animals. Nine sets of PCR primers for regional markers with an average distance of 15 kb between each primer were used to confirm the integrity of the transgene in the five transgenic lines carrying the HLA‐DPA1/DPB1 gene. Transgene copy numbers were determined by real‐time PCR analysis. HLA‐DP401 gene expression was analyzed at the mRNA and protein level. Although infection with S aureus Newman did not alter the percentage of immune cells in the spleen and thymus from the HLA‐DP401‐H2‐Aβ1 humanized mice. Increased expression of HLA‐DP401 was observed in the thymus of the humanized mice infected by S aureus . Conclusions We generated several BAC transgenic mice, and analyzed the expression of HLA‐DPA1/DPB1 in those mice. A model of S aureus ‐induced pneumonia in the HLA‐DP401‐H2‐Aβ1 −/− humanized mice was further developed, and S aureus infection upregulated the HLA‐DP401 expression in thymus of those humanized mice. These findings demonstrate the potential of those HLA‐DPA1/DPB1 transgenic humanized mice for developing animal models of infectious diseases and MHC‐associated immunological diseases.
    Type of Medium: Online Resource
    ISSN: 2576-2095 , 2576-2095
    URL: Article
    DOI: 10.1002/ame2.12158
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 3009615-7
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  • 5
    Online Resource
    Online Resource
    Development of a humanized HLA‐A30 transgenic mouse model
    Wiley ; 2022
    In:  Animal Models and Experimental Medicine Vol. 5, No. 4 ( 2022-08), p. 350-361
    Details
    In: Animal Models and Experimental Medicine, Wiley, Vol. 5, No. 4 ( 2022-08), p. 350-361
    Abstract: There are remarkable genetic differences between animal major histocompatibility complex (MHC) systems and the human leukocyte antigen (HLA) system. HLA transgenic humanized mouse model systems offer a much better method to study the HLA‐A‐related principal mechanisms for vaccine development and HLA‐A‐restricted responses against infection in human. Methods A recombinant gene encoding the chimeric HLA‐A30 monochain was constructed. This HHD molecule contains the following: α1‐α2 domains of HLA‐A30, α3 and cytoplasmic domains of H‐2D b , linked at its N‐terminus to the C‐terminus of human β2m by a 15‐amino‐acid peptide linker. The recombinant gene encoding the chimeric HLA‐A30 monochain cassette was introduced into bacterial artificial chromosome (BAC) CH502‐67J3 containing the HLA‐A01 gene locus by Red‐mediated homologous recombination. Modified BAC CH502‐67J3 was microinjected into the pronuclei of wild‐type mouse oocytes. This humanized mouse model was further used to assess the immune responses against influenza A virus (H1N1) pdm09 clinically isolated from human patients. Immune cell population, cytokine production, and histopathology in the lung were analyzed. Results We describe a novel human β2m‐HLA‐A30 (α1α2)‐H‐2D b (α3 transmembrane cytoplasmic) (HHD) monochain transgenic mouse strain, which contains the intact HLA‐A01 gene locus including 49 kb 5′‐UTR and 74 kb 3′‐UTR of HLA‐A01*01. Five transgenic lines integrated into the large genomic region of HLA‐A gene locus were obtained, and the robust expression of exogenous transgene was detected in various tissues from A30‐18# and A30‐19# lines encompassing the intact flanking sequences. Flow cytometry revealed that the introduction of a large genomic region in HLA‐A gene locus can influence the immune cell constitution in humanized mice. Pdm09 infection caused a similar immune response among HLA‐A30 Tg humanized mice and wild‐type mice, and induced the rapid increase of cytokines, including IFN‐γ, TNF‐α, and IL‐6, in both HLA‐A30 humanized Tg mice and wild‐type mice. The expression of HLA‐A30 transgene was dramatically promoted in tissues from A30‐9# line at 3 days post‐infection (dpi). Conclusions We established a promising preclinical research animal model of HLA‐A30 Tg humanized mouse, which could accelerate the identification of novel HLA‐A30‐restricted epitopes and vaccine development, and support the study of HLA‐A‐restricted responses against infection in humans.
    Type of Medium: Online Resource
    ISSN: 2576-2095 , 2576-2095
    URL: Issue
    URL: Article
    DOI: 10.1002/ame2.v5.4
    DOI: 10.1002/ame2.12225
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 3009615-7
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  • 6
    Online Resource
    Online Resource
    High cerebrospinal fluid lactate concentration at 48 h of hospital admission predicts poor outcomes in patients with tuberculous meningitis: A multicenter retrospective cohort study
    Frontiers Media SA ; 2022
    In:  Frontiers in Neurology Vol. 13 ( 2022-10-6)
    Details
    In: Frontiers in Neurology, Frontiers Media SA, Vol. 13 ( 2022-10-6)
    Abstract: This study aimed to analyze the cerebrospinal fluid (CSF) parameters affecting the outcomes of patients with tuberculous meningitis (TBM). Methods This is a multi-center, retrospective, cohort study involving 81 patients who were diagnosed with TBM and treated in Haihe Clinical College of Tianjin Medical University, Tianjin Medical University General Hospital, and General Hospital of Air Force PLA from January 2016 to December 2019. Baseline data, Glasgow Coma Scale (GCS) score, and clinical presentations of all patients were collected at admission. CSF samples were collected at 48 h, 1, 2, and 3 weeks after admission. CSF lactate, adenosine deaminase, chloride, protein, glucose levels and intracranial pressure were measured. After a follow-up of 16.14 ± 3.03 months, all patients were assessed using the modified Rankin Scale (mRS) and divided into good (mRS scores of 0–2 points) and poor outcome groups (mRS scores of 3–6 points). The differences in patients' baseline data, GCS score, clinical presentations, and levels of CSF parameters detected at 48 h, 1, 2, and 3 weeks after admission between two groups were compared. Statistically significant variables were added to the binary logistic regression model to identify the factors impacting the outcomes of patients with TBM. Receiver operating characteristic (ROC) curve was used to assess the predictive ability of the model. Results The CSF lactate level exhibited a decreasing trend within 3 weeks of admission in the two groups. For the within-group comparison, statistically significant differences in the lactate level was found in both groups between four different time points. A binary logistic regression model revealed that CSF lactate level at 48 h after admission, age, and GSC score on admission were independently associated with the outcomes of patients with TBM. ROC curve analysis showed that the area under the ROC curve (AUC) was 0.786 for the CSF lactate level (48 h), 0.814 for GCS score, and 0.764 for age. Conclusion High CSF lactate level at 48 h after admission is one of the important factors for poor outcomes in patients with TBM.
    Type of Medium: Online Resource
    ISSN: 1664-2295
    URL: Article
    DOI: 10.3389/fneur.2022.989832
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2564214-5
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  • 7
    Online Resource
    Online Resource
    Robust transmission expansion planning model considering multiple uncertainties and active load
    Elsevier BV ; 2021
    In:  Global Energy Interconnection Vol. 4, No. 5 ( 2021-10), p. 476-484
    Details
    In: Global Energy Interconnection, Elsevier BV, Vol. 4, No. 5 ( 2021-10), p. 476-484
    Type of Medium: Online Resource
    ISSN: 2096-5117
    URL: Article
    DOI: 10.1016/j.gloei.2021.11.009
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2969898-4
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  • 8
    Online Resource
    Online Resource
    Dual-input two-compartment pharmacokinetic model of dynamic contrast-enhanced magnetic resonance imaging in hepatocellular carcinoma
    Baishideng Publishing Group Inc. ; 2016
    In:  World Journal of Gastroenterology Vol. 22, No. 13 ( 2016), p. 3652-
    Details
    In: World Journal of Gastroenterology, Baishideng Publishing Group Inc., Vol. 22, No. 13 ( 2016), p. 3652-
    Type of Medium: Online Resource
    ISSN: 1007-9327
    URL: Article
    DOI: 10.3748/wjg.v22.i13.3652
    Language: English
    Publisher: Baishideng Publishing Group Inc.
    Publication Date: 2016
    detail.hit.zdb_id: 2084831-6
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  • 9
    Online Resource
    Online Resource
    Prognostic model for predicting overall survival in patients with glioblastoma: an analysis based on the SEER database
    SAGE Publications ; 2023
    In:  Journal of Investigative Medicine Vol. 71, No. 4 ( 2023-04), p. 439-447
    Details
    In: Journal of Investigative Medicine, SAGE Publications, Vol. 71, No. 4 ( 2023-04), p. 439-447
    Abstract: Predicting the prognosis of glioblastoma (GBM) has always been important for improving survival. An understanding of the prognostic factors for patients with GBM can help guide treatment. Herein, we aimed to construct a prognostic model for predicting overall survival (OS) for patients with GBM. We identified 11,375 patients with pathologically confirmed GBM from the Surveillance, Epidemiology, and End Results database between 2004 and 2015. The 1-, 2-, and 3-year survival probabilities were 48.8%, 22.5%, and 13.1%, respectively. The patients were randomly divided into the training cohort (n = 8531) and the validation cohort (n = 2844). A Cox proportional risk regression model was used to analyze the prognostic factors of patients in the training cohort, and a nomogram was constructed. Then concordance indexes (C-indexes), calibration curves, and receiver operating characteristic (ROC) curves were used to assess the performance of the nomograms by internal (training cohort) and external validation (validation cohort). Log-rank test and univariate analysis showed that age, race, marital status, extent of surgical resection, chemotherapy, and radiation were the prognostic factors for patients with GBM (p  〈  0.05), which were used to construct nomogram. The C-index of the nomogram was 0.717 (95% confidence interval (CI), 0.710–0.724) in the training cohort, and 0.724 (95% CI, 0.713–0.735) in the validation cohort. The nomogram had a higher areas under the ROC curve value. The nomogram was well validated, which can effectively predict the OS of patients with GBM. Thus, this nomogram could be applied in clinical practice.
    Type of Medium: Online Resource
    ISSN: 1081-5589 , 1708-8267
    URL: Article
    DOI: 10.1177/10815589221147153
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
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  • 10
    Online Resource
    Online Resource
    Neurotization of the phrenic nerve with accessory nerve for high cervical spinal cord injury with respiratory distress: an anatomic study
    Turkish Neurosurgical Society ; 2013
    In:  Turkish Neurosurgery ( 2013)
    Details
    In: Turkish Neurosurgery, Turkish Neurosurgical Society, ( 2013)
    Type of Medium: Online Resource
    ISSN: 1019-5149
    URL: Article
    DOI: 10.5137/1019-5149.JTN.8335-13.1
    Language: Unknown
    Publisher: Turkish Neurosurgical Society
    Publication Date: 2013
    detail.hit.zdb_id: 2433666-X
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