In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 12 ( 2021-10-14)
Abstract:
Soil salinity is one of the major factors that limit the area of cultivable land and yield potential of crops. The ability of salt tolerance varies with plant species. Peanut ( Arachis hypogaea L.) is a moderately salt-sensitive and economically important crop, however, their biological processes involved in salt-stress response remain unclear. In this study, we investigated the role of A. hypogaea L. ABSCISIC ACID INSENSITIVE 4s ( AhABI4s ) in salt tolerance and elucidated its mode of action in peanuts. The results showed that the downregulation of AhABI4 s via whole plant virus-induced gene silencing has enhanced the survival rate, biomass accumulation, and root/shoot ratio of peanut seedlings in response to salt-stress. Transcriptomics, quantitative proteomics, and phosphoproteomic analyses were performed using AhABI4 s-silenced and Mock plants. The expression pattern of 15,247 genes, 1,900 proteins, and 2,620 phosphorylation sites were affected by silencing of AhABI4 s in peanut leaf and root after sodium chloride (NaCl) treatment. Among them, 63 potential downstream target genes of ABI4 changed consistently at both transcription and translation levels, and the protein/phosphorylation levels of 31 ion transporters/channels were also affected. Electrophoretic mobility shift assays (EMSA) showed that ABI4 was able to bind to the promoters of HSP70, fructokinase ( FRK ), and pyruvate kinase ( PK ) coding genes in vitro . In addition, we also detected a binding preference of AhABI4 for CACT(G/T)GCA motif in the promoters of down-regulated genes in peanut leaf. Collectively, the potential downstream targets which were regulated at the levels of transcription and translation, binding preference, and in vivo phosphorylation sites that had been revealed in this study will provide new insight into the AhABI4s -mediated salt tolerance regulation mechanism in peanuts.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2021.741641
DOI:
10.3389/fpls.2021.741641.s001
DOI:
10.3389/fpls.2021.741641.s002
DOI:
10.3389/fpls.2021.741641.s003
DOI:
10.3389/fpls.2021.741641.s004
DOI:
10.3389/fpls.2021.741641.s005
DOI:
10.3389/fpls.2021.741641.s006
DOI:
10.3389/fpls.2021.741641.s007
DOI:
10.3389/fpls.2021.741641.s008
DOI:
10.3389/fpls.2021.741641.s009
DOI:
10.3389/fpls.2021.741641.s010
DOI:
10.3389/fpls.2021.741641.s011
DOI:
10.3389/fpls.2021.741641.s012
DOI:
10.3389/fpls.2021.741641.s013
DOI:
10.3389/fpls.2021.741641.s014
DOI:
10.3389/fpls.2021.741641.s015
DOI:
10.3389/fpls.2021.741641.s016
DOI:
10.3389/fpls.2021.741641.s017
DOI:
10.3389/fpls.2021.741641.s018
DOI:
10.3389/fpls.2021.741641.s019
DOI:
10.3389/fpls.2021.741641.s020
DOI:
10.3389/fpls.2021.741641.s021
DOI:
10.3389/fpls.2021.741641.s022
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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