In:
Journal of Phytopathology, Wiley, Vol. 161, No. 7-8 ( 2013-08), p. 536-543
Abstract:
Downy mildew ( DM ), caused by Pseudoperonospora cubensis (Berk. & M.A. Curtis) Rostovzev, is a worldwide major disease of cucumbers ( Cucumis sativus L.). By screening 10 introgression lines ( IL s) derived from interspecific hybridization between cucumber and the wild Cucumis , C. hystrix , through a whole plant assay, one introgression line ( IL 52) was identified with high DM ‐resistance. IL 52 was further used as a resistant parent to make an F 2 population with ‘changchunmici’ (susceptible parent). The F 2 population (300 plants) was investigated for DM ‐yellowing, DM ‐necrosis and DM ‐resistance in the adult stage. A genetic map spanning 642.5 cM with 104 markers was constructed and used for QTL analysis from the population. Three QTL regions were identified on chromosome 5 and chromosome 6. By interval mapping analysis, two QTL s for DM ‐resistance were determined on chromosome 5 (DM_5.1 and DM_5.2), which explained 17.9% and 14.2% of the variation, respectively. QTL s for DM ‐yellowing were in the same regions as DM ‐resistance. For DM ‐necrosis, by interval mapping analysis, one QTL was determined on chromosome 5 (Necr_5.1) that explained 18.3% of the variation and one on chromosome 6 (Necr_6.1) that explained 13.9% of the variation. Our results indicated that the identification of molecular markers linked to the QTL s could be further applied for marker‐assisted selection ( MAS ) of downy mildew resistance in cucumber.
Type of Medium:
Online Resource
ISSN:
0931-1785
,
1439-0434
DOI:
10.1111/jph.2013.161.issue-7pt8
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2020539-9
SSG:
12
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