In:
The Journal of Immunology, The American Association of Immunologists, Vol. 194, No. 1_Supplement ( 2015-05-01), p. 129.1-129.1
Abstract:
Co-receptors, being either co-stimulatory or co-inhibitory, play a pivotal role in T cell immunity, as they decide the fate of T cell function. CD43 is one of the abundant cell surface glycoproteins expressed on T cells. CD43 has been demonstrated to act as not only a potent co-receptor but also a negative regulator for T cell activation. To further investigate role of CD43 in T cell activation and subsequent function, peripheral blood T cells were activated via two distinct CD43 epitopes recognized by monoclonal antibodies (mAbs) 6E5 (T6E5-act) and 10G7 (T10G7-act) along with TCR signalling. Both the CD43 mAbs could potently induce T cell activation when immobilized but exerted differential downstream effects including differential activation of signalling pathways, T cell cytokine production and also distinct effector function. T10G7-act poorly responded in re-stimulation assay and further acquired suppressive function compared to T6E5-act or to T cells activated via CD28 (TCD28-act). Furthermore, T10G7-act did not directly inhibit responder T cells, but rather exhibited their effect via dendritic cells when added to allogenic mixed leukocyte reaction. Together our data suggests a unique role of CD43 in bidirectional polarization of T cell immunity, a positive co-stimulatory role and a negative role in down-modulation of immune response, depending on its targeted epitope.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.194.Supp.129.1
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2015
detail.hit.zdb_id:
1475085-5
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