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  • 1
    In: Frontiers in Physiology, Frontiers Media SA, Vol. 8 ( 2017-09-22)
    Type of Medium: Online Resource
    ISSN: 1664-042X
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2017
    detail.hit.zdb_id: 2564217-0
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  • 2
    In: LaboratoriumsMedizin, Walter de Gruyter GmbH, Vol. 41, No. 5 ( 2017-10-26), p. 229-237
    Abstract: In der Sportmedizin und -wissenschaft sowie im Hochleistungssport werden Untersuchungen sowohl unter standardisierten Bedingungen im Labor als auch im Feld durchgeführt. Es kommen dabei die unterschiedlichsten medizinischen Messmethoden zum Einsatz. Fast immer werden sie von Blutanalysen begleitet, wobei sowohl hochkomplexe Laborverfahren als auch das Point of care Testing (POCT) angewendet werden. Auch wenn das POCT schon mit Beginn seiner Entwicklung im sportlichen Kontext Beachtung gefunden hat, so ist der Begriff in diesem Bereich noch nicht etabliert und Veröffentlichungen von Untersuchungen mit Leistungs- und Spitzensportlern, bei denen das POCT als Messmethode explizit genannt wird, bisher sehr selten. Der vorliegende Artikel soll aus diesem Grund an Hand unterschiedlicher Studien und in Anlehnung an einen Vortrag auf dem 3. Münchener POCT-Symposium einen Überblick über die verschiedenen Fragestellungen mit sportwissenschaftlichem Hintergrund bieten, bei denen POCT zur athletennahen Sofortdiagnostik eingesetzt wird.
    Type of Medium: Online Resource
    ISSN: 1439-0477 , 0342-3026
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2017
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    detail.hit.zdb_id: 2909042-8
    SSG: 15,3
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  • 3
    In: Medicina, MDPI AG, Vol. 57, No. 7 ( 2021-06-29), p. 673-
    Abstract: Background and Objectives: During intense training periods, there is a high need to monitor the external and especially the internal training load in order to fine-tune the training process and to avoid overreaching or overtraining. However, data on stress reactions, especially of biomarkers, to high training loads in children and youth are rare. Therefore, in this study, we aimed to investigate the training load of youth athletes during a training camp using a multilevel approach. Materials and Methods: Six trained youth male cyclists performed a 7-day preseason training camp. To investigate the internal training load, every morning, minimally invasive “point-of-care testing” (POCT) devices were used to analyze the following biomarkers: creatine kinase (CK), blood urea nitrogen (BUN), albumin (Alb), bilirubin (Bil), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total protein (TP). Additionally, data of training load measures (HR: heart rate, RPE: rating of perceived exertion, sRPE: session-RPE, TRIMP: training impulse, intensity (RPE:HR), and load (sRPE:TRIMP) ratios), self-perception (person’s perceived physical state, questionnaires on muscle soreness, and sleep quality), and measures of the autonomic nervous system (resting heart rate, heart rate variability) were collected. Two days before and after the training camp, subjects performed performance tests (Graded Exercise Test, Wingate Anaerobic Test, Counter Movement Jump). Results: Primarily, the biomarkers CK, BUN, and Alb, as well as the self-perception showed moderate to large load-dependent reactions during the 7-day training camp. The biomarkers returned to baseline values two days after the last training session. Power output at lactate threshold showed a small increase, and no changes were found for other performance parameters. Conclusions: The study suggests that a multilevel approach is suitable to quantify the internal training load and that different parameters can be used to control the training process. The biomarkers CK, BUN, and Alb are suitable for objectively quantifying the internal training load. The self-perception provides additional subjective information about the internal training load.
    Type of Medium: Online Resource
    ISSN: 1648-9144
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2088820-X
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  • 4
    In: Pflügers Archiv, Springer Science and Business Media LLC, Vol. 415, No. 1 ( 1990-12), p. R1-R119
    Type of Medium: Online Resource
    ISSN: 0031-6768 , 1432-2013
    RVK:
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 1990
    detail.hit.zdb_id: 1463014-X
    SSG: 12
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  • 5
    In: Nature, Springer Science and Business Media LLC, Vol. 513, No. 7518 ( 2014-9), p. 409-413
    Type of Medium: Online Resource
    ISSN: 0028-0836 , 1476-4687
    RVK:
    RVK:
    RVK:
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2014
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    detail.hit.zdb_id: 1413423-8
    SSG: 11
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  • 6
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 72, No. 8_Supplement ( 2012-04-15), p. 3526-3526
    Abstract: Blinatumomab, a CD19/CD3-bispecific BiTE antibody has shown high response rates and durable remissions in patients with relapsed or refractory acute lymphocytic leukemia and non-Hodgkin's lymphoma. For treatment of patients with prostate cancer (PCa), we have developed a novel BiTE antibody, MT112/BAY2010112, that is bispecific for prostate-specific membrane antigen (PSMA) and the CD3 epsilon subunit of the T cell receptor complex. MT112/BAY 2010112 binds PSMA and CD3 of human and macaque origin allowing for assessment of safety, pharmacodynamics and pharmacokinetics in a relevant animal species. PSMA expressing human PCa cell lines VCaP, 22Rv1, MDA PCa 2b, C4-2, PC3-PSMA and LNCaP were lysed by freshly isolated, unstimulated human T cells redirected by MT112/BAY2010112 at EC50 values ranging between 0.1 and 4 ng/ml (1.8-72 pM). Efficacy of lysis correlated with levels of surface expressed PSMA. No lysis was observed in PSMA-negative PCa cell lines PC3 and DU145, showing the target antigen-specific activity of MT112/BAY2010112. The relevance of cynomolgus monkeys for further nonclinical safety evaluation of MT112/BAY 2010112 was demonstrated by in vitro side-by-side comparison of the BiTE antibody's pharmacological characteristics in human and cynomolgus monkey assay systems. For studying the activity of MT112/BAY 2010112 after subcutaneous (s.c.) administration, human 22Rv1 PCa xenografts were grown s.c. in mice to sizes of & gt;200 mm3 in the absence of human T cells. Three days prior to treatment with BiTE, ex-vivo expanded human T cells were intraperitoneally injected. Animals were treated s.c. at 2.5 mg/kg/d or intravenously (i.v.) at 0.5 mg/kg/d for 28 consecutive days. S.c. administration of MT112/BAY 2010112 resulted in a similar drug exposure, rapid shrinkage and complete remission of established PCa xenografts as seen upon i.v. administration of the BiTE antibody. Treatment with human T cells or vehicle alone had no effect on tumor growth. Compared to i.v. administration, the relative bioavailability of the PSMA BiTE in serum after s.c. injection in mice was approximately 18%. Initial results will be presented showing that 14C-labeled MT112/BAY 2010112 accumulates in s.c. implanted LNCaP PCa tumors of mice after tail vein injection. Based on these preclinical in vivo pharmacology results, treatment of PCa patients with s.c. administered MT112/BAY2010112 appears to be justified. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3526. doi:1538-7445.AM2012-3526
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    RVK:
    RVK:
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2012
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
    detail.hit.zdb_id: 410466-3
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  • 7
    In: ChemBioChem, Wiley, Vol. 7, No. 10 ( 2006-10-06), p. 1605-1611
    Abstract: In addition to the transmembrane protein, GP 1,2 , the Ebola virus glycoprotein gene encodes the soluble glycoproteins sGP and Δ‐peptide. Two more soluble proteins, GP 1 and GP 1,2 Δ TM , are generated from GP 1,2 as a result of disulfide‐bond instability and proteolytic cleavage, respectively, and are shed from the surface of infected cells. The sGP glycoprotein is secreted as a disulfide‐linked homodimer, but there have been conflicting reports on whether it is arranged in a parallel or antiparallel orientation. Off‐line HPLC‐MALDI‐TOF MS (MS/MS) was used to identify the arrangement of all disulfide bonds and simultaneously determine site‐specific information regarding N ‐glycosylation. Our data prove that sGP is a parallel homodimer that contains C53–C53’ and C306–C306’ disulfide bonds, and although there are six predicted N ‐linked carbohydrate sites, only five are consistently glycosylated. The disulfide bond arrangement was confirmed by using cysteine to glycine mutations at amino acid positions 53 and 306. The mutants had a reduced ability to rescue the barrier function of TNF‐ α‐ treated endothelial cells—a function previously reported for sGP. This indicates that these disulfide bonds are critical for the proposed anti‐inflammatory function of sGP.
    Type of Medium: Online Resource
    ISSN: 1439-4227 , 1439-7633
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2006
    detail.hit.zdb_id: 2020469-3
    SSG: 12
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  • 8
    In: Archiv der Pharmazie, Wiley, Vol. 321, No. 1 ( 1988-01), p. 21-24
    Abstract: 2,6‐Piperidinediones, VI: The Anticholinergic Activities of the Enantiomere of 2,6‐Piperidinediones with Basic Side Chain The anticholinergic activities of the enantiomers of the piperidine‐2,6‐diones 1 , 2 and 3 , which are disubstituted at position 3 and have a basic side chain, were tested on the isolated ileum of rats. Only the dextrorotatory enantiomers show anticholinergic activity in the dose range used. The activity decreases depending on the cyclic 3‐substituents from the phenyl over the thienyl to the cyclohexyl group. Determination of the inhibition type was made graphically by transformation of the dosis‐response curves according to Lineweaver‐Burk . A competitive antagonism was found.
    Type of Medium: Online Resource
    ISSN: 0365-6233 , 1521-4184
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 1988
    detail.hit.zdb_id: 1496815-0
    SSG: 15,3
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  • 9
    Online Resource
    Online Resource
    Wiley ; 2018
    In:  American Journal of Physical Anthropology Vol. 166, No. 1 ( 2018-05), p. 246-260
    In: American Journal of Physical Anthropology, Wiley, Vol. 166, No. 1 ( 2018-05), p. 246-260
    Abstract: The purpose of this study was to put forth a precise landmark‐based technique for reconstructing the three‐dimensional shape of human entheseal surfaces, to investigate whether the shape of human entheses is related to their size. The effects of age‐at‐death and bone length on entheseal shapes were also assessed. Materials and methods The sample comprised high‐definition three‐dimensional models of three right hand entheseal surfaces, which correspond to 45 male adult individuals of known age. For each enthesis, a particular landmark configuration was introduced, whose precision was tested both within and between observers. The effect of three‐dimensional size, age‐at‐death, and bone length on shape was investigated through shape regression. Results The method presented high intra‐observer and inter‐observer repeatability. All entheses showed significant allometry, with the area of opponens pollicis demonstrating the most substantial relationship. This was particularly due to variation related to its proximal elongated ridge. The effect of age‐at‐death and bone length on entheses was limited. Discussion The introduced methodology can set a reliable basis for further research on the factors affecting entheseal shape. Using both size and shape, variables can provide further information on entheseal variation and its biomechanical implications. The low entheseal variation by age verifies that specimens under 50 years of age are not substantially affected by age‐related changes. The lack of correlation between entheseal shape and bone length or age implies that other factors may regulate entheseal surfaces. Future research should focus on multivariate shape patterns among entheses and their association with occupation.
    Type of Medium: Online Resource
    ISSN: 0002-9483 , 1096-8644
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1495833-8
    detail.hit.zdb_id: 3129801-1
    SSG: 12
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  • 10
    In: American Journal of Physical Anthropology, Wiley, Vol. 157, No. 3 ( 2015-07), p. 458-471
    Abstract: Objectives: Straight next to a segment of the outer ditch of the Late Neolithic Michelsberg Culture earthwork of Bruchsal‐Aue in SW‐Germany (ca. 4250–3650 calBC), a multiple burial of eight individuals (two male adults and six children) plus a subsequent child burial was excavated. In this study, we applied a multidisciplinary approach to elucidate interpersonal relationships and life histories within this collective. Materials and methods: To determine the identity of this collective, we performed aDNA analyses in addition to osteological examination using HVR I plus Y‐chromosomal and autosomal STR profiling to find evidence for kinship relations. Strontium isotopic analyses were used to reconsider migrational behavior. To find evidence for a specific social affiliation, the individual diet was reconstructed by performing nitrogen and carbon isotopic analyses. Furthermore, radiocarbon‐dating was carried out to integrate the burial context into an absolute timeframe. Two nearby single burials were included in the analyses for comparison. Results: Because of a shared HVR I haplotype, three pairs of individuals were most likely linked by kinship, and statistical testing on autosomal STR profiles shows a high probability for the pair of two men being brothers. Although it cannot be excluded, isotopic data gave no clear proof for migration. A rather poor health status is indicated by skeletal stress markers even though the isotope data attest to a diet rich in meat and fish. Discussion: Although clear kinship relations among the infants remain unconfirmed, a relationship could also be indicated by the positioning of the bodies in the burial pit. Whereas a common cause of death might have been the presupposition for their special treatment, interpersonal relationships were likely the decisive factor for the multiple burial. Am J Phys Anthropol 157:458–471, 2015. © 2015 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 0002-9483 , 1096-8644
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1495833-8
    detail.hit.zdb_id: 3129801-1
    SSG: 12
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