In:
New Phytologist, Wiley, Vol. 219, No. 1 ( 2018-07), p. 149-162
Abstract:
Whilst WRKY transcription factors are known to be involved in diverse plant responses to biotic stresses, their involvement in abiotic stress tolerance is poorly understood. Os FRDL 4 , encoding a citrate transporter, has been reported to be regulated by ALUMINUM (Al) RESISTANCE TRANSCRIPTION FACTOR 1 ( ART 1) in rice, but whether it is also regulated by other transcription factors is unknown. We define the role of Os WRKY 22 in response to Al stress in rice by using mutation and transgenic complementation assays, and characterize the regulation of Os FRDL 4 by Os WRKY 22 via yeas one‐hybrid, electrophoretic mobility shift assay and Ch IP ‐quantitative PCR. We demonstrate that loss of Os WRKY 22 function conferred by the oswrky22 T‐ DNA insertion allele causes enhanced sensitivity to Al stress, and a reduction in Al‐induced citrate secretion. We next show that Os WRKY 22 is localized in the nucleus, functions as a transcriptional activator and is able to bind to the promoter of Os FRDL 4 via W‐box elements. Finally, we find that both Os FRDL 4 expression and Al‐induced citrate secretion are significantly lower in art1 oswrky22 double mutants than in the respective single mutants. We conclude that Os WRKY 22 promotes Al‐induced increases in Os FRDL 4 expression, thus enhancing Al‐induced citrate secretion and Al tolerance in rice.
Type of Medium:
Online Resource
ISSN:
0028-646X
,
1469-8137
DOI:
10.1111/nph.2018.219.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
208885-X
detail.hit.zdb_id:
1472194-6
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