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  • 1
    Online Resource
    Online Resource
    Gut microbiota composition may relate to weight loss rate in obese pet dogs
    Wiley ; 2017
    In:  Veterinary Medicine and Science Vol. 3, No. 4 ( 2017-11), p. 252-262
    Details
    In: Veterinary Medicine and Science, Wiley, Vol. 3, No. 4 ( 2017-11), p. 252-262
    Abstract: Obese dogs seem to have a different gut microbiome ( GM ) composition compared to lean dogs, and in humans, GM composition may negatively impact the ability to lose weight in some individuals. The purpose of this study was to investigate the interaction between exercise, weight‐loss and the composition of GM in dogs. Eighteen obese pet dogs were recruited for a 12‐week weight‐loss intervention. All dogs were fed restrictively with a commercial high‐protein/high‐fibre dry diet, and eight of these dogs were enrolled in an exercise program in addition to the diet intervention. Faecal samples were collected and the dogs were weighed at week 0, week 6 and week 12. GM composition was determined using MiSeq‐based tag‐encoded 16S rRNA gene high‐throughput amplicon sequencing, and concentrations of short chain fatty acids ( SCFA ) by gas‐liquid chromatography. Total weight loss, food allowance and GM were not changed by exercise inclusion. However, Megamonas abundance negatively correlated with weight loss rate and Ruminococcaceae relative abundance was lower at 12 weeks in dogs with a faster weight loss rate (≥1% per week) compared with slower weight loss rate ( 〈 1% per week) independent of exercise. Acetic and propionic acid concentrations decreased in the dogs with a faster weight loss rate. Members of Megamonas and Ruminococcaceae produce acetic and propionic acids and we therefore interpret that having a GM that favour SCFA production may negatively affect weight loss rate in dogs. Weight loss rate in dogs may be related to the composition of the GM and its production of metabolites.
    Type of Medium: Online Resource
    ISSN: 2053-1095 , 2053-1095
    URL: Issue
    URL: Article
    DOI: 10.1002/vms3.2017.3.issue-4
    DOI: 10.1002/vms3.80
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2819409-3
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  • 2
    Online Resource
    Online Resource
    Analytical performance of canine acylated and unacylated ghrelin enzyme‐linked immunosorbent assays
    Wiley ; 2019
    In:  Veterinary Clinical Pathology Vol. 48, No. 4 ( 2019-12), p. 748-753
    Details
    In: Veterinary Clinical Pathology, Wiley, Vol. 48, No. 4 ( 2019-12), p. 748-753
    Abstract: Ghrelin is a major appetite‐stimulating hormone. It circulates as acylated ghrelin (AG) and unacylated ghrelin (UAG), which could have different metabolic actions in obesity. Our objective was to study the analytical performance of two new canine AG and UAG ELISAs using blood samples from healthy, normal‐weight dogs. Additionally, the effect of a protease inhibitor (PI) on short‐term sample storage was analyzed. Methods The intra‐ and inter‐assay precision for low, intermediate, and high AG and UAG concentrations, accuracy, limits of quantification (LQ), and detection limit (DL) of a blank sample were determined in ten healthy dogs. To study the effects of a PI on ghrelin concentrations, and AG and UAG concentrations were compared in five canine plasma samples stored for 1 month with (PI+), without (PI−), and with PI added at sample thawing (PI+th). Results The intra‐ and inter‐assay coefficients of variation were 1.8%‐5.7% and 2.9%‐6.4% for the AG assay, and 0.8%‐7.5% and 2.8%‐13.4% for the UAG assay, respectively. Accuracy analyses showed nonsignificant deviation from linearity for the AG ( R 2  = .99; Runs test: P  = .37) and UAG ( R 2  = .99; Runs test: P  = .42) assays. For the AG assay, the upper LQ was 〉 1261 pg/mL, the lower LQ was 6.2 pg/mL, and the DL was 0.3 pg/mL. For the UAG assay, the upper LQ was 〉 1785 pg/mL, the lower LQ was 16.3 pg/mL, and the DL was 1.8 pg/mL. No differences in the AG ( P  = .54) and UAG ( P  = .95) concentrations were detected in the plasma samples subjected to PI+, PI−, and PI+th. Conclusion The AG and UAG ELISA assays had acceptable precision, accuracy, lower LQ, and DLs, but upper LQ could not be established. An influence of the PI on short‐term storage was not detectable. Long‐term storage ± PI was not evaluated and should be investigated further.
    Type of Medium: Online Resource
    ISSN: 0275-6382 , 1939-165X
    URL: Issue
    URL: Article
    DOI: 10.1111/vcp.v48.4
    DOI: 10.1111/vcp.12801
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2114702-4
    SSG: 22
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  • 3
    Online Resource
    Online Resource
    Simple Video-Based Spatiotemporal Gait Analysis Is Not Better than Subjective Visual Assessment of Lameness in Dogs
    Georg Thieme Verlag KG ; 2021
    In:  VCOT Open Vol. 04, No. 01 ( 2021-01), p. e65-e71
    Details
    In: VCOT Open, Georg Thieme Verlag KG, Vol. 04, No. 01 ( 2021-01), p. e65-e71
    Abstract: Introduction Visual gait analysis is prone to subjectivity, but objective analysis systems are not widely available to clinicians. Simple video analysis using high-definition recordings might enable identification of temporal or spatial variations that could permit objective and repeatable assessments of lameness in general practice. Methods Cohorts of normal and mildly to moderately lame dogs were filmed using a standardized protocol. Using freely available software, measurements of stance, swing and stride time were obtained, along with measurements of pelvic, shoulder, and head height for each limb. Symmetry ratios were calculated, and distributions of normal and lame dogs compared using Mann–Whitney U test and Kruskal–Wallis test. Results Recordings from 35 normal dogs were assessed along with 30 dogs with grade 1 to 3/5 lameness. While no consistent significant differences in temporal characteristics could be found, head height asymmetry was significantly different between lame and normal dogs (p = 0.003), with pairwise comparison showing this difference was restricted to forelimb-lame dogs (p = 0.03). Conclusion While potentially useful for patient records, use of video recordings at walking speeds for simple spatiotemporal gait analysis does not appear to offer clinically significant advantages over visual gait analysis in a typical clinical population of lame dogs.
    Type of Medium: Online Resource
    ISSN: 2625-2325
    URL: Article
    DOI: 10.1055/s-0041-1731437
    Language: English
    Publisher: Georg Thieme Verlag KG
    Publication Date: 2021
    detail.hit.zdb_id: 2934191-7
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  • 4
    Online Resource
    Online Resource
    Integration of a physical training program in a weight loss plan for overweight pet dogs
    American Veterinary Medical Association (AVMA) ; 2016
    In:  Journal of the American Veterinary Medical Association Vol. 248, No. 2 ( 2016-01-15), p. 174-182
    Details
    In: Journal of the American Veterinary Medical Association, American Veterinary Medical Association (AVMA), Vol. 248, No. 2 ( 2016-01-15), p. 174-182
    Abstract: OBJECTIVE To investigate whether a controlled physical training plan for overweight dogs during a weight loss program would improve cardiorespiratory fitness and better preserve lean body mass, compared with results for dogs undergoing a weight loss program based on caloric restriction alone. DESIGN Prospective, nonrandomized clinical study. ANIMALS 19 client-owned overweight or obese dogs. PROCEDURES All dogs were fed the same calorie-restricted diet rationed to achieve a weight loss rate of 1% to 2%/wk for 12 weeks. The fitness-and-diet (FD) group participated in a training program that included underwater and land-based treadmill exercise 3 times/wk. The diet-only (DO) group had no change in exercise routines. Daily activity before and during the intervention was recorded by accelerometry. Before and after intervention, heart rate during exercise was recorded to assess cardiovascular fitness, and body composition was analyzed by dual-energy x-ray absorptiometry. Differences between groups were evaluated with t tests and multiple regression analysis. RESULTS Mean weight loss was 13.9% and 12.9% for the FD and DO groups, respectively (n = 8 dogs/group that completed the study). Mean accelerometer counts during intervention were 13% higher than baseline counts for the FD group. Heart rate during exercise declined after intervention in both groups. Lean body mass was preserved in the FD group and lost in the DO group during intervention. CONCLUSIONS AND CLINICAL RELEVANCE The controlled exercise plan used with a dietary weight loss program prevented loss of lean body mass in dogs. This finding supports inclusion of controlled physical training for obesity management in dogs.
    Type of Medium: Online Resource
    ISSN: 0003-1488
    URL: Article
    DOI: 10.2460/javma.248.2.174
    Language: Unknown
    Publisher: American Veterinary Medical Association (AVMA)
    Publication Date: 2016
    detail.hit.zdb_id: 2904887-4
    SSG: 22
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  • 5
    Online Resource
    Online Resource
    Physical training and weight loss in dogs lead to transcriptional changes in genes involved in the glucose-transport pathway in muscle and adipose tissues
    Elsevier BV ; 2016
    In:  The Veterinary Journal Vol. 208 ( 2016-02), p. 22-27
    Details
    In: The Veterinary Journal, Elsevier BV, Vol. 208 ( 2016-02), p. 22-27
    Type of Medium: Online Resource
    ISSN: 1090-0233
    URL: Article
    DOI: 10.1016/j.tvjl.2015.11.002
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2016
    detail.hit.zdb_id: 2002624-9
    SSG: 22
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