In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 45, No. 4 ( 2007-04), p. 1255-1260
Abstract:
In order to assess the applicability of multiplexed fluorescence in situ hybridization (FISH) assay for the clinical setting, we conducted retrospective analysis of 110 formalin-stored diarrheic stool samples from human immunodeficiency virus (HIV)/AIDS patients with intestinal microsporidiosis collected between 1992 and 2003. The multiplexed FISH assay identified microsporidian spores in 94 of 110 (85.5%) samples: 49 (52.1%) were positive for Enterocytozoon bieneusi , 43 (45.8%) were positive for Encephalitozoon intestinalis , 2 (2.1%) were positive for Encephalitozoon hellem , and 9 samples (9.6%) contained both E. bieneusi and E. intestinalis spores. Quantitative spore counts per ml of stool yielded concentration values from 3.5 × 10 3 to 4.4 × 10 5 for E. bieneusi (mean, 8.8 × 10 4 /ml), 2.3 × 10 2 to 7.8 × 10 4 (mean, 1.5 × 10 4 /ml) for E. intestinalis , and 1.8 × 10 2 to 3.6 × 10 2 for E. hellem (mean, 2.7 × 10 2 /ml). Identification of microsporidian spores by multiplex FISH assay was more sensitive than both Chromotrope-2R and CalcoFluor White M2R stains; 85.5% versus 72.7 and 70.9%, respectively. The study demonstrated that microsporidian coinfection in HIV/AIDS patients with intestinal microsporidiosis is not uncommon and that formalin-stored fecal samples older than 10 years may not be suitable for retrospective analysis by techniques targeting rRNA. Multiplexed FISH assay is a reliable, quantitative fluorescence microscopy method for the simultaneous identification of E. bieneusi , E. intestinalis , and E. hellem , as well as Encephalitozoon cuniculi , spores in fecal samples and is a useful tool for assessing spore shedding intensity in intestinal microsporidiosis. The method can be used for epidemiological investigations and applied in clinical settings.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.01975-06
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2007
detail.hit.zdb_id:
1498353-9
SSG:
12
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