In:
Physiological Research, Institute of Physiology of the Czech Academy of Sciences
Abstract:
Gastrointestinal form is the second stage of the Acute Radiation
Syndrome (ARS) with a threshold dose of 8 Gy. It represents an absolutely lethal clinical-pathological unit, enteritis necrohemorrhagica (duodenitis, jejunitis, ileitis, respectively) with
unknown causal therapy. The purpose of our study has been to evaluate the morphological changes in a model of radiationinduced enteritis in rats and estimate the significance of changes
in biodosimetry. Wistar rats were randomly divided into 21 groups, 10 animals per group. Samples of the jejunum were
taken 24, 48, 72, and 96 h after the whole-body γ-irradiation with the doses of 1, 5, 10, 15, and 20 Gy, and routinely stained
with hematoxylin and eosin. Five morphometric markers – intercryptal distance, enterocytal height on the top and base of
villus, length of basal lamina of 10 enterocytes and enterocytal
width – in irradiated rat jejunum were examined. The results were compared with sham-irradiated control group. After lethal
doses of irradiation, all morphometric parameters of jejunum significantly changed. With the exception of intercryptal distance,
they might be considered as suitable biodosimetric markers under these experimental conditions. Our morphometry results in
radiation-induced jejunitis are in accordance with those in other studies. We were the first who quantified morphological postirradiation changes in animal jejunum. Some of them might be
used under experimental conditions. This experimental study is a predecessor of the clinical assessment of a specific marker.
Under clinical practice, the sensitive biodosimetric parameter could serve as one of the guidance for evaluation of the
absorbed dose in irradiated troops as well as rescue workers. This is in accordance with tasks and Standardization Agreement
of the North Atlantic Treaty Organization.
Type of Medium:
Online Resource
ISSN:
1802-9973
,
0862-8408
DOI:
10.33549/physiolres.931027
Language:
English
Publisher:
Institute of Physiology of the Czech Academy of Sciences
Publication Date:
2008
detail.hit.zdb_id:
2100162-5
detail.hit.zdb_id:
1073141-6
SSG:
12
Permalink