In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-09-05)
Abstract:
Adherens junction (AJ) assembly under force is essential for many biological processes like epithelial monolayer bending, collective cell migration, cell extrusion and wound healing. The acto-myosin cytoskeleton acts as a major force-generator during the de novo formation and remodeling of AJ. Here, we investigated the role of non-muscle myosin II isoforms (NMIIA and NMIIB) in epithelial junction assembly. NMIIA and NMIIB differentially regulate biogenesis of AJ through association with distinct actin networks. Analysis of junction dynamics, actin organization, and mechanical forces of control and knockdown cells for myosins revealed that NMIIA provides the mechanical tugging force necessary for cell-cell junction reinforcement and maintenance. NMIIB is involved in E-cadherin clustering, maintenance of a branched actin layer connecting E-cadherin complexes and perijunctional actin fibres leading to the building-up of anisotropic stress. These data reveal unanticipated complementary functions of NMIIA and NMIIB in the biogenesis and integrity of AJ.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.46599.001
DOI:
10.7554/eLife.46599.002
DOI:
10.7554/eLife.46599.005
DOI:
10.7554/eLife.46599.003
DOI:
10.7554/eLife.46599.004
DOI:
10.7554/eLife.46599.006
DOI:
10.7554/eLife.46599.007
DOI:
10.7554/eLife.46599.008
DOI:
10.7554/eLife.46599.010
DOI:
10.7554/eLife.46599.009
DOI:
10.7554/eLife.46599.011
DOI:
10.7554/eLife.46599.012
DOI:
10.7554/eLife.46599.017
DOI:
10.7554/eLife.46599.013
DOI:
10.7554/eLife.46599.014
DOI:
10.7554/eLife.46599.015
DOI:
10.7554/eLife.46599.016
DOI:
10.7554/eLife.46599.018
DOI:
10.7554/eLife.46599.019
DOI:
10.7554/eLife.46599.020
DOI:
10.7554/eLife.46599.022
DOI:
10.7554/eLife.46599.021
DOI:
10.7554/eLife.46599.023
DOI:
10.7554/eLife.46599.030
DOI:
10.7554/eLife.46599.024
DOI:
10.7554/eLife.46599.025
DOI:
10.7554/eLife.46599.026
DOI:
10.7554/eLife.46599.027
DOI:
10.7554/eLife.46599.028
DOI:
10.7554/eLife.46599.029
DOI:
10.7554/eLife.46599.031
DOI:
10.7554/eLife.46599.032
DOI:
10.7554/eLife.46599.035
DOI:
10.7554/eLife.46599.036
DOI:
10.7554/eLife.46599.034
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2019
detail.hit.zdb_id:
2687154-3
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