In:
Tropical and Subtropical Agroecosystems, Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia, Vol. 24, No. 2 ( 2021-04-13)
Abstract:
〈 p 〉 〈 strong 〉 Background. 〈 /strong 〉 The edible mushroom 〈 em 〉 Pleurotus ostreatus 〈 /em 〉 is a potential alternative for the control of gastrointestinal nematodes due to their nematoxins and secondary metabolites. 〈 strong 〉 Objective. 〈 /strong 〉 To evaluate the 〈 em 〉 in vitro 〈 /em 〉 anthelmintic activity of hydroalcoholic extracts of mycelium, basidiomata and spent substrate of the edible mushroom 〈 em 〉 P. ostreatus 〈 /em 〉 against eggs and unsheathed larvae of 〈 em 〉 Haemonchus contortus 〈 /em 〉 . 〈 strong 〉 Methodology. 〈 /strong 〉 The mycelium of 〈 em 〉 P. ostreatus 〈 /em 〉 was extracted with ethanol–water (70:30) and the basidiomata and the spent substrate with methanol–water (70:30) which were concentrated to obtain the mycelium (MHA), basidiomata (BHA) and spent mushroom substrate extract (SMSE) extracts. The eggs and larvae of 〈 em 〉 H. contortus 〈 /em 〉 were exposed to different extracts in 96-well plates. The tests used were the egg-hatch and larval mortality tests. Three exposure times, 24, 48 and 72 h, were considered. The data were analyzed with a factorial design and the comparison of means by Duncan test. 〈 strong 〉 Results. 〈 /strong 〉 The percentage anthelmintic effectiveness of the mycelium hydroalcoholic extract (MHA) of 〈 em 〉 P. ostreatus 〈 /em 〉 against eggs and larvae of 〈 em 〉 H. contortus 〈 /em 〉 were 93 and 96.8% at 12.8 and 240 mg/mL (respectively) after 72 h. Also, activity was observed for SMSE and BHA against eggs, with values of 87.2 and 100%, respectively, at 25.6 mg/mL after 72 h. However, SMSE showed no activity against 〈 em 〉 H. contortus 〈 /em 〉 larvae, while the BHA extract showed less than 70% corresponding activity. 〈 strong 〉 Implications. 〈 /strong 〉 This was an exploratory study of 〈 em 〉 in vitro 〈 /em 〉 tests; the 〈 em 〉 in vivo 〈 /em 〉 studies are still needed. 〈 strong 〉 Conclusion. 〈 /strong 〉 The MHA extract of 〈 em 〉 P. ostreatus 〈 /em 〉 showed anthelmintic activity against the eggs and larvae of 〈 em 〉 H. contortus 〈 /em 〉 . 〈 /p 〉
Type of Medium:
Online Resource
ISSN:
1870-0462
Language:
Unknown
Publisher:
Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia
Publication Date:
2021
detail.hit.zdb_id:
2486562-X
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