In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-20), p. e0247227-
Abstract:
In humans, GART [phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) / phosphoribosylglycinamide synthetase (EC 6.3.4.13) / phosphoribosylaminoimidazole synthetase (EC 6.3.3.1)] is a trifunctional protein which catalyzes the second, third, and fifth reactions of the ten step de novo purine synthesis (DNPS) pathway. The second step of DNPS is conversion of phosphoribosylamine (5-PRA) to glycineamide ribonucleotide (GAR). 5-PRA is extremely unstable under physiological conditions and is unlikely to accumulate in the absence of GART activity. Recently, a HeLa cell line null mutant for GART was constructed via CRISPR-Cas9 mutagenesis. This cell line, crGART, is an important cellular model of DNPS inactivation that does not accumulate DNPS pathway intermediates. In the current study, we characterized the crGART versus HeLa transcriptomes in purine-supplemented and purine-depleted growth conditions. We observed multiple transcriptome changes and discuss pathways and ontologies particularly relevant to Alzheimer disease and Down syndrome. We selected the Cluster of Differentiation (CD36) gene for initial analysis based on its elevated expression in crGART versus HeLa as well as its high basal expression, high log2 value, and minimal P-value.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0247227
DOI:
10.1371/journal.pone.0247227.g001
DOI:
10.1371/journal.pone.0247227.g002
DOI:
10.1371/journal.pone.0247227.g003
DOI:
10.1371/journal.pone.0247227.g004
DOI:
10.1371/journal.pone.0247227.g005
DOI:
10.1371/journal.pone.0247227.g006
DOI:
10.1371/journal.pone.0247227.g007
DOI:
10.1371/journal.pone.0247227.g008
DOI:
10.1371/journal.pone.0247227.g009
DOI:
10.1371/journal.pone.0247227.s001
DOI:
10.1371/journal.pone.0247227.s002
DOI:
10.1371/journal.pone.0247227.s003
DOI:
10.1371/journal.pone.0247227.s004
DOI:
10.1371/journal.pone.0247227.s005
DOI:
10.1371/journal.pone.0247227.s006
DOI:
10.1371/journal.pone.0247227.s007
DOI:
10.1371/journal.pone.0247227.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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