In:
Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam, Vietnamese Journal of Food Control, National Institute for Food Control, Vol. 4, No. 1 ( 2021-02-05), p. 15-17
Abstract:
A method for the simultaneous determination of Nisin A and Nisin Z in nutritional products by LC-MS/MS has been developed. Sample treatment was performed by ultrasonic extraction using a mixture of 0.1 M CH3COONH4 buffer solution - 1.0 M NaCl (pH 2.0): MeOH (1:1, v/v) at room temperature for 10 minutes. Extracts were then cleaned through C18 solid phase extraction column (500 mg, 3 mL) and analyzed on LC-MS/MS instrument using a C18 column (100 mm & times; 2 mm & times; 1.7 & micro;m) with ESI (+) mode. The method was validated for linear calibration curve in the range of 10 - 1,000 & micro;g/L; relative standard deviation (RSD) 2.12 - 5.77%; recovery 80.1 - 105%, meeting AOAC requirements. The method has been applied to analyze 25 samples of nutritious food collected from the local markets (including: liquid milk, powdered milk, butter, cheese, nutritious powder), showing that two samples were detected both Nisin A and Nisin Z.
Type of Medium:
Online Resource
ISSN:
2615-9252
DOI:
10.47866/2615-9252/vjfc.2772
Language:
Unknown
Publisher:
Vietnamese Journal of Food Control, National Institute for Food Control
Publication Date:
2021
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