In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 10 ( 2022-10-4), p. e3001813-
Abstract:
The reduced sleep duration previously observed in Camk2b knockout mice revealed a role for Ca 2+ /calmodulin-dependent protein kinase II (CaMKII)β as a sleep-promoting kinase. However, the underlying mechanism by which CaMKIIβ supports sleep regulation is largely unknown. Here, we demonstrate that activation or inhibition of CaMKIIβ can increase or decrease sleep duration in mice by almost 2-fold, supporting the role of CaMKIIβ as a core sleep regulator in mammals. Importantly, we show that this sleep regulation depends on the kinase activity of CaMKIIβ. A CaMKIIβ mutant mimicking the constitutive-active (auto)phosphorylation state promotes the transition from awake state to sleep state, while mutants mimicking subsequent multisite (auto)phosphorylation states suppress the transition from sleep state to awake state. These results suggest that the phosphorylation states of CaMKIIβ differently control sleep induction and maintenance processes, leading us to propose a “phosphorylation hypothesis of sleep” for the molecular control of sleep in mammals.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001813
DOI:
10.1371/journal.pbio.3001813.g001
DOI:
10.1371/journal.pbio.3001813.g002
DOI:
10.1371/journal.pbio.3001813.g003
DOI:
10.1371/journal.pbio.3001813.g004
DOI:
10.1371/journal.pbio.3001813.g005
DOI:
10.1371/journal.pbio.3001813.g006
DOI:
10.1371/journal.pbio.3001813.s001
DOI:
10.1371/journal.pbio.3001813.s002
DOI:
10.1371/journal.pbio.3001813.s003
DOI:
10.1371/journal.pbio.3001813.s004
DOI:
10.1371/journal.pbio.3001813.s005
DOI:
10.1371/journal.pbio.3001813.s006
DOI:
10.1371/journal.pbio.3001813.s007
DOI:
10.1371/journal.pbio.3001813.s008
DOI:
10.1371/journal.pbio.3001813.s009
DOI:
10.1371/journal.pbio.3001813.s010
DOI:
10.1371/journal.pbio.3001813.s011
DOI:
10.1371/journal.pbio.3001813.s012
DOI:
10.1371/journal.pbio.3001813.s013
DOI:
10.1371/journal.pbio.3001813.s014
DOI:
10.1371/journal.pbio.3001813.s015
DOI:
10.1371/journal.pbio.3001813.s016
DOI:
10.1371/journal.pbio.3001813.s017
DOI:
10.1371/journal.pbio.3001813.s018
DOI:
10.1371/journal.pbio.3001813.s019
DOI:
10.1371/journal.pbio.3001813.s020
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
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