In:
Journal of Bacteriology, American Society for Microbiology, Vol. 180, No. 10 ( 1998-05-15), p. 2770-2774
Kurzfassung:
Temperature-induced changes in thermotolerance and protein composition were examined in heat-shocked cells and high-temperature-grown cells of the extremely thermophilic bacterium Rhodothermus obamensis . The survival at temperatures superoptimal for growth (90 and 95°C) was enhanced in both heat-shocked cells and high-temperature-grown cells relative to that of cells grown at optimal temperatures. In a comparison of protein composition using two-dimensional gel electrophoresis, putative heat shock proteins (HSPs) and high-temperature growth-specific proteins (HGPs) were detected. N-terminal amino acid sequence analysis revealed that the putative HSPs were quite similar to the ATP-binding subunits of ABC transporters and the HGPs were proteins corresponding to domains II and III of elongation factor Tu. These results suggested that this extreme thermophile has developed temperature-induced responses that include increased survival under hyperthermal conditions, changes in protein composition, and also the production of novel HSPs.
Materialart:
Online-Ressource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/JB.180.10.2770-2774.1998
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
1998
ZDB Id:
1481988-0
SSG:
12
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