In:
Proceedings, annual meeting, Electron Microscopy Society of America, Cambridge University Press (CUP), Vol. 36, No. 2 ( 1978-08), p. 662-663
Abstract:
The histological identification of the malignancy of a cartilaginous tumor is not always simple. The ultrastructural structure of the cartilage has been described in numerous reports but there are few dealing with chondrosarcoma. Present authors have been studying to discover definite characteristics of chondrosarcoma. In this study, we hope to discover the characteristics of chondrosarcoma by concentrating on the collagen fibers which are one of the main components of chondroid tumor. Ten cases of chondrosarcoma ranging from 14 to 65 years of age, were examined using both transmission electron microscope and scanning electron microscope, and their structures were compared with other chondroid tissues, taken from non-malignant cartilaginous tumor, normal conditioning articular cartilage, and the meniscus of the knee joint. Small pieces of the specimens were fixed in 3% glutaraldehyde buffered with Millonig's phosphate buffer, post-fixed in 1% osmium tetroxide and embedded in Epon 812. Sections cut by LKB ultramicrotome were stained by uranyl acetate and lead citrate and examined with an AKASHI-TRS-80 electron microscope. The preparations for the scanning electron microscopy were coated with carbon and gold after the critical point drying method.
Type of Medium:
Online Resource
ISSN:
0424-8201
,
2690-1315
DOI:
10.1017/S0424820100084181
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
1978
SSG:
11
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