In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 7 ( 2023-7-28), p. e0289073-
Abstract:
Dietary fat can alter host metabolism and gut microbial composition. Crocodile oil (CO) was extracted from the fatty tissues of Crocodylus siamensis . CO, rich in monounsaturated- and polyunsaturated fatty acids, has been reported to reduce inflammation, counter toxification, and improve energy metabolism. The aim of this study was to investigate the effect of CO on gut microbiota (GM) in laboratory mice as well as the accompanying metabolic changes in the animals. Forty-five C57BL/6 male mice were randomly divided into five groups and orally administrated either sterile water (control [C]); 1 or 3% (v/w) CO (CO-low [CO-L] and CO-high [CO-H], respectively); or 1 or 3% (v/w) palm oil (PO-low and PO-high, respectively) for 11 weeks. Body weight gain, food intake, energy intake, blood glucose levels, and blood lipid profiles were determined. Samples from colon tissue were collected and the 16S rRNA genes were pyrosequenced to clarify GM analyses. The results showed that there were no differences in body weight and blood glucose levels. Food intake by the mice in the CO-L and CO-H groups was statistically significantly less when compared to that by the animals in the C group. However, neither CO treatment had a statistically significant effect on calorie intake when compared to the controls. The CO-H exhibited a significant increase in serum total cholesterol and low-density lipoprotein but showed a downward trend in triglyceride levels compared to the control. The GM analyses revealed that both CO treatments have no significant influence on bacterial diversity and relative abundance at the phylum level, whereas increases of Choa1 and abundance-based coverage estimator indexes, distinct β-diversity, and Proteobacteria abundance were observed in the PO-high group compared with the C group. Furthermore, the abundance of Azospirillum thiophilum and Romboutsia ilealis was significantly higher in the CO-L and CO-H groups which could be associated with energy metabolic activity. Thus, CO may be an alternative fat source for preserving host metabolism and gut flora.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0289073
DOI:
10.1371/journal.pone.0289073.g001
DOI:
10.1371/journal.pone.0289073.g002
DOI:
10.1371/journal.pone.0289073.g003
DOI:
10.1371/journal.pone.0289073.g004
DOI:
10.1371/journal.pone.0289073.g005
DOI:
10.1371/journal.pone.0289073.g006
DOI:
10.1371/journal.pone.0289073.t001
DOI:
10.1371/journal.pone.0289073.t002
DOI:
10.1371/journal.pone.0289073.s001
DOI:
10.1371/journal.pone.0289073.s002
DOI:
10.1371/journal.pone.0289073.s003
DOI:
10.1371/journal.pone.0289073.s004
DOI:
10.1371/journal.pone.0289073.r001
DOI:
10.1371/journal.pone.0289073.r002
DOI:
10.1371/journal.pone.0289073.r003
DOI:
10.1371/journal.pone.0289073.r004
DOI:
10.1371/journal.pone.0289073.r005
DOI:
10.1371/journal.pone.0289073.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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