In:
Angewandte Chemie, Wiley, Vol. 126, No. 46 ( 2014-11-10), p. 12706-12710
Abstract:
Conventional dissociation‐enhanced lanthanide fluoroimmunoassays (DELFIA) using molecular probes suffer from a low labeling ratio of lanthanide ions (Ln 3+ ) per biomolecule. Herein, we develop a unique bioassay based on the dissolution‐enhanced luminescence of inorganic lanthanide nanoparticles (NPs). As a result of the highly concentrated Ln 3+ ions in a single Ln 3+ NP, an extremely high Ln 3+ labeling ratio can be achieved, which amplifies significantly the luminescence signal and thus improves the detection sensitivity compared to DELFIA. Utilizing sub‐10 nm NaEuF 4 NPs as dissolution‐enhanced luminescent nanoprobes, we demonstrate the successful in vitro detection of carcinoembryonic antigen (CEA, an important tumor marker) in human serum samples with a record‐low detection limit of 0.1 pg mL −1 (0.5 f M ). This value is an improvement of approximately 3 orders of magnitude relative to that of DELFIA. The dissolution‐enhanced luminescent bioassay shows great promise in versatile bioapplications, such as ultrasensitive and multiplexed in vitro detection of disease markers in clinical diagnosis.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v126.46
DOI:
10.1002/ange.201405937
Language:
German
Publisher:
Wiley
Publication Date:
2014
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