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  • 1
    Online Resource
    Online Resource
    Diagnostic significance of TLR2 and TLR4 receptors on lymphoid cells as a marker of the progression of periodontal inflammation associated with key periodontal pathogenic species 〈i〉F. alocis〈/i〉 and 〈i〉P. gingivalis〈/i〉
    Central Research Institute for Epidemiology ; 2022
    In:  Journal of microbiology, epidemiology and immunobiology Vol. 99, No. 5 ( 2022-12-07), p. 565-572
    Details
    In: Journal of microbiology, epidemiology and immunobiology, Central Research Institute for Epidemiology, Vol. 99, No. 5 ( 2022-12-07), p. 565-572
    Abstract: The aim of the work was to evaluate the diagnostic value of TLR2 and TLR4 expression on periodontal and peripheral blood lymphoid cells by immunofluorescence microscopy in patients with chronic periodontitis associated with key periodontal pathogenic species Filifactor alocis, Porphyromonas gingivalis. Materials and methods. The study included 150 patients 88 (59%) women and 62 (41%) men aged 18 to 73 years with chronic periodontitis in the acute phase (CP) and 32 people without signs of chronic periodontal inflammation. To confirm the diagnosis of periodontitis, the Multident-5 PCR kit was used (detection of P. gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans), as well as rt-PCR for F. alocis and P. gingivalis in the contents of the periodontal pocket (NPF GenLab, Russia). To evaluate cells carrying CD282 and CD284 markers, gingival fluid flushes from the periodontal pocket with Hanks' solution were used. The isolated cells were stained with antibodies to CD282 markers (corresponding to TLR2 receptor) or CD284 (corresponding to TLR4 receptor) labeled with FITC, and fixed with paraformaldehyde for subsequent immunofluorescence microscopy. Results. The expression of TLR2 and TLR4 on peripheral blood and gingival fluid leukocytes was studied in individuals with healthy periodontitis and patients with chronic periodontitis associated with F. alocis, P. gingivalis. According to the results of PCR, the detection rate of F. alocis and P. gingivalis was 64 and 62.7%, respectively, which confirmed their dominance in the microbial association. It was found that the expression of TLR2 and TLR4 on peripheral blood lymphoid cells varied in humans. The possible diagnostic significance of this phenomenon in assessing the progression of chronic periodontitis is discussed. Conclusion. In patients with chronic periodontitis associated with the dominance of periodontopathogenic species F. alocis, P. gingivalis, the multidirectional expression of TLR2 and TLR4 on peripheral blood cells was observed, which may have diagnostic significance in assessing the progression of periodontal diseases.
    Type of Medium: Online Resource
    ISSN: 2686-7613 , 0372-9311
    URL: Article
    DOI: 10.36233/0372-9311-336
    Language: Unknown
    Publisher: Central Research Institute for Epidemiology
    Publication Date: 2022
    SSG: 12
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  • 2
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    Online Resource
    Sensitivity of parodontopatogens to the cell juice of Siberian fir (microbiological investigation)
    ECO-Vector LLC ; 2022
    In:  Russian Journal of Dentistry Vol. 25, No. 6 ( 2022-07-22), p. 505-510
    Details
    In: Russian Journal of Dentistry, ECO-Vector LLC, Vol. 25, No. 6 ( 2022-07-22), p. 505-510
    Abstract: BACKGROUND: Search for new effective means of preventing inflammatory complications in the state of peri-implant tissues is relevant; in particular, products from coniferous greens produced by the Solagift company (Tomsk) are of interest. The bioeffective cell juice of the Siberian fir, the 100% carbon dioxide extract of Siberian fir (water fraction), stands out among them. Fir cell juice is recommended for enhancing immunity, preventing inflammatory diseases of the upper respiratory tract, and treating several pathological conditions. Fir juice contains vitamin C, carotene, polyphenolic compounds, flavonoids, iron, magnesium, copper, manganese, zinc, and maltoliron chelate complex (a natural antioxidant). AIM: This microbiological study aimed to examine the sensitivity of periodontal pathogens to bioeffective cell juice of Siberian fir of various concentrations. MATERIAL AND METHODS: A number of periodontal pathogens were cultivated in the presence of bioeffective Siberian fir cell juice in the following proportions: 1:5, 1:10, and 1:15. Incubation for up to 3 days was conducted in a reverse-spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density (OD) of the culture at a wavelength () of 850 nm. OD was measured in McFarland units (mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontal pathogens: adaptive (lag phase), exponential (log phase), stationary, and withering away. The following clinical isolates of microorganisms were used: Streptococcus constellatus, Staphylococcus aureus, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. RESULTS: The microbiological study of the sensitivity of periodontal pathogens (such as S. constellatus, S. aureus, F. nucleatum, and A. actinomycetemcomitans) to bioeffective cell juice of Siberian fir showed a growth slowdown of microorganisms in the presence of fir juice and inhibition of the growth of a culture of periodontal pathogens when co-cultivated with fir juice. A significant decrease in the OD of cultures of all studied periodontal pathogens was observed at a fir juice concentration of 1:10 and 1: 5. At these concentrations, there was no growth of S. aureus culture, and the degree of decrease in the average OD of other periodontal pathogens reached 27.1% (A. actinomycetemcomitans), 31.8% (F. nucleatum), and 33.6% (S. constellatus).
    Type of Medium: Online Resource
    ISSN: 2413-2934 , 1728-2802
    URL: Article
    DOI: 10.17816/1728-2802-2021-25-6-505-510
    Language: Unknown
    Publisher: ECO-Vector LLC
    Publication Date: 2022
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  • 3
    Online Resource
    Online Resource
    Effect of the oil-ether complex of Siberian fir on the fungal and periodontopathogenic flora of the mouth (microbiological study)
    ECO-Vector LLC ; 2022
    In:  Russian Journal of Dentistry Vol. 26, No. 2 ( 2022-09-04), p. 89-94
    Details
    In: Russian Journal of Dentistry, ECO-Vector LLC, Vol. 26, No. 2 ( 2022-09-04), p. 89-94
    Abstract: BACKGROUND: Peri-implantitis is mainly caused by the activity of periodontal pathogens with insufficient oral hygiene, which causes the disintegration of intraosseous dental implants against the background of chronic inflammation in peri-implant tissues. The correct techniques in the post-prosthetic stage in patients with dental implants include medical examination with regular professional oral hygiene, which is complicated by low patient compliance. In this regard, there are high requirements for the level of individual oral hygiene and for the effectiveness of local hygiene products, among which dental rinses occupy an important place. As rinses, it is possible to use pine-containing substances, particularly a complex of oil-essential fir Solagift of a Siberian company (Tomsk). AIM: To conduct a microbiological study of the sensitivity of periodontopathogens and Candida albicans to the oil-essential complex of Siberian fir of different concentrations. MATERIAL AND METHODS: A number of periodontopathogens and C. albicans were cultured in the presence of a complex of oilessential Siberian fir in the following proportions: 1:5, 1:10, and 1:15. Incubation for up to 3 days was conducted in the Reverse Spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density of the culture (OD) at a wavelength of =850 nm. The optical density was measured in McFarland units (Mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontopathogens: adaptive (lag phase), exponential (log phase), stationary, and dying. The following clinical isolates of microorganisms were used: Streptococcus constellatus, Staphylococcus aureus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and C. albicans. RESULTS: Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5 by 13.727.1% (A. actinomycetemcomitans), 18.362.0% (F. nucleatum), 30.056.4% (S. aureus), and 19.274.1% (S. constellatus). The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%. CONCLUSION. Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5. The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%. The bacteriostatic effectiveness of the Siberian fir oil-ether complex significantly exceeds the effect of the herbal preparation Stomatophyte.
    Type of Medium: Online Resource
    ISSN: 2413-2934 , 1728-2802
    URL: Article
    DOI: 10.17816/1728-2802-2022-26-2-89-94
    Language: Unknown
    Publisher: ECO-Vector LLC
    Publication Date: 2022
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  • 4
    Online Resource
    Online Resource
    The First Domestic Experience of Detecting the Association of Anaerobic Bacteria 〈i〉Filifactor Alocis〈/i〉 and 〈i〉Porphyromonas Gingivalis〈/i〉 by Molecular Biological Methods in Periodontal Diseases and Comorbid Pathology (Comparative Research)
    Paediatrician Publishers LLC ; 2023
    In:  Annals of the Russian academy of medical sciences Vol. 77, No. 6 ( 2023-02-04), p. 437-446
    Details
    In: Annals of the Russian academy of medical sciences, Paediatrician Publishers LLC, Vol. 77, No. 6 ( 2023-02-04), p. 437-446
    Abstract: Background. The widespread global increase in the incidence of periodontitis and the role of its pathogens in comorbid pathology and systemic complications determines the need to create new molecular genetic systems for diagnosis and the use of metagenomic and bioinformatic analysis methods. Aims to use methods of microbiological genodiagnostics and bioinformatic analysis to prove the etiological role of the key periodontal pathogens Filifactor alocis and Porphyromonas gingivalis, characterizing the degree of progression of chronic periodontitis, and its association with a systemic pathological process (type 2 diabetes mellitus). Methods. A comparative assessment of the identification of key periodontopathogenic species P. gingivalis and F. alocis in different forms of periodontitis according to the degree of progression (84 people) using a previously patented system of primers in patients in 4 comparison groups, differing in the degree of progression. 16S sequencing and bioinformatic analysis were performed in 69 patients (including 38 with type 2 diabetes mellitus). All nondiabetic patients were required to have HbA1c level 6.0%. Results. A higher frequency of detection of periodontal pathogens was established in patients of group C with a pronounced tendency to progression (93 and 100% respectively). The simultaneous presence of P. gingivalis and F. alocis in chronic periodontitis of grade B was noted in 20% of cases, and in grade C in 93% of cases. Conclusions. The proposed method can be used to effectively determine the degree of periodontitis progression based on the determination of oligonucleotide primers of P. gingivalis and F. alocis, including comorbid pathology periodontitis and type 2 diabetes mellitus.
    Type of Medium: Online Resource
    ISSN: 2414-3545 , 0869-6047
    URL: Issue
    URL: Article
    DOI: 10.15690/vramn.776
    DOI: 10.15690/vramn2262
    Language: Unknown
    Publisher: Paediatrician Publishers LLC
    Publication Date: 2023
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  • 5
    Online Resource
    Online Resource
    Fig. 7. Percentage analysis of the inoculation of representatives of microorganisms isolated from autogenous dentinal blocks in patients with various types of antiseptic treatment (with n strain=25, p 〈0.05).
    ECO-Vector LLC ; 2023
    In:  Russian Journal of Dentistry Vol. 27, No. 3 ( 2023-08-23), p. 219-228
    Details
    In: Russian Journal of Dentistry, ECO-Vector LLC, Vol. 27, No. 3 ( 2023-08-23), p. 219-228
    Abstract: BACKGROUND: Autogenous material is currently one of the most common and effective materials used for bone grafting. In recent years, materials based on extracted teeth have attracted attention. When analyzing modern articles, all authors agree that before using autogenous dentinal material, it is necessary to carry out antiseptic treatment to prevent possible complications. AIM: A comparison was made of various antiseptic agents for the treatment of autogenous dentinal blocks with subsequent osteoplastic operations in the pre-implantation period. MATERIALS AND METHODS: At the Department of Maxillofacial and Plastic Surgery of the Moscow State University of Medicine and Dentistry named after A.I. Evdokimov, 43 teeth were removed for orthodontic indications, the teeth were processed mechanically and fragmented into 125 autogenous dentinal blocks. Then they were treated with antiseptic agents (0.01% Miramistin solution, 95% ethyl alcohol solution, 0.05% and 2% chlorhexidine solution) for 15 min and were sent to the Department of Microbiology, Virology, Immunology of the Moscow State University of Medicine and Dentistry named after A.I. Evdokimov for the isolation of microbial cultures. RESULTS: The use of antiseptic agents showed a significant reduction in microbial contamination compared with the control group, which did not undergo antiseptic treatment, which indicates the effectiveness of the treatment of extracted teeth before using it as an osteoplastic material for bone grafting. CONCLUSION: Autogenous dentinal blocks must be processed before being used as an osteoplastic material.
    Type of Medium: Online Resource
    ISSN: 2413-2934 , 1728-2802
    URL: Issue
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.17816/dent.2023.27.3
    DOI: 10.17816/dent321610
    DOI: 10.17816/dent321610-3680851
    DOI: 10.17816/dent321610-3680852
    DOI: 10.17816/dent321610-3680853
    DOI: 10.17816/dent321610-3680854
    DOI: 10.17816/dent321610-3680855
    DOI: 10.17816/dent321610-3680856
    DOI: 10.17816/dent321610-3680857
    Language: Unknown
    Publisher: ECO-Vector LLC
    Publication Date: 2023
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  • 6
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    Online Resource
    Technology of the discator strip seeding. Condition, prospects
    EDP Sciences ; 2021
    In:  E3S Web of Conferences Vol. 273 ( 2021), p. 01030-
    Details
    In: E3S Web of Conferences, EDP Sciences, Vol. 273 ( 2021), p. 01030-
    Abstract: The article is devoted to the implementation of strip sowing technology with a discator working body. The strip sowing, in comparison with the existing row sowing, increases the area of seed feeding, which increases the yield of grain crops. The coulters currently used for strip sowing with the under-spreading distributor are energy intensive and prone to clogging. It is proposed to use disc spherical disc coulters with a distributor. Compact designs of disc seed drills with spherical disc openers have been developed, which can replace bulky seeding complexes. Disc seed drills combine strip sowing with mini and no-till, reducing energy consumption, increasing speeds and productivity. Providing seeds with nutritional areas of at least 10 cm2, they actually implement a new technology for sowing cereals, which can increase yields by 2-3 times compared to ordinary sowing.
    Type of Medium: Online Resource
    ISSN: 2267-1242
    URL: Article
    DOI: 10.1051/e3sconf/202127301030
    Language: English
    Publisher: EDP Sciences
    Publication Date: 2021
    detail.hit.zdb_id: 2755680-3
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