In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 71, No. 8_Supplement ( 2011-04-15), p. 2870-2870
Abstract:
Propolis has been used empirically for centuries and it was always mentioned as an immunomodulatory agent. Propolis has over 150 constituents such as polyphenols (flavonoids, phenolic acids and their esters), terpenoids, steroids, and amino acids, but its composition varies qualitatively and quantitatively with the geographical and botanical origins. In Tawiwan, there are ten propolins (A-J) were isolated and characterized from the local propolins. Propolins, prenylflavanones are isolated and characterized from Taiwanese propolis have been shown inhibits certain cancer cell lines growth and induced apoptosis. In this study, human lung adenocarcinoma cell (A549) was used to investigate the anticancer activity of Proploins. Propolin C and propolin G could inhibit A549 cell growth by dose-dependent manner. The combination of propolin C with propolin G was more predominantly inhibited the cell viability. Propolin C or propolin G treatment alone could suppress Akt and p70S6K activation by blocking the PI3K/Akt/mTOR pathway. Moreover, the pro-apoptotic protein Bax and Bad expression were increased. The procaspase 9, procaspase 3, and PARP activated in cascade to lead to mitochondria-mediated apoptosis pathway of apoptosis. Flow cytometry assay showed that sub-G1 phase increased significantly in propolin G treatment but not in propolin C group. Both propolin C and G could promote LC3B increased within 6 hours treatment, moreover, and AVOs (acidic vesicular organelles) significantly increased by AO (Acridine orange) staining. Combination treatment had synergies effect to induce significantly apoptosis and autophagy in A549 cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2870. doi:10.1158/1538-7445.AM2011-2870
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2011-2870
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2011
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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