GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Material
Publisher
Person/Organisation
Language
Years
FID
Subjects(RVK)
  • 1
    Online Resource
    Online Resource
    A potential protein-based vaccine for influenza H5N1 from the recombinant HA1 domain of avian influenza A/H5N1 expressed in Pichia pastoris
    Future Medicine Ltd ; 2014
    In:  Future Virology Vol. 9, No. 12 ( 2014-12), p. 1019-1031
    Details
    In: Future Virology, Future Medicine Ltd, Vol. 9, No. 12 ( 2014-12), p. 1019-1031
    Abstract: ABSTRACT  The HA1 genes from influenza A strains A/Puerto Rico/8/1934 H1N1 (A/PR/8/34) and A/Hatay/2004 H5N1 were each cloned in Pichia pastoris vectors in the correct reading frame with the yeast α-factor secretion signal and the C-terminus His-tag, resulting in simple, fast purification of expressed H1HA1 and H5HA1 protein from the culture medium. Mice vaccinated with the purified proteins showed robust T cell, anti-HA1 IgG responses and developed a high antibody response for hemagglutination inhibition (HI) at titer 7.6 log 2 . Chickens vaccinated with a dose of 200 µg of H5HA1 mixed with either Montanide or Freund's adjuvants gave HI values of up to 7 log 2 at the third week comparable with a licensed inactivated H5N1 vaccine.
    Type of Medium: Online Resource
    ISSN: 1746-0794 , 1746-0808
    URL: Article
    DOI: 10.2217/fvl.14.93
    Language: English
    Publisher: Future Medicine Ltd
    Publication Date: 2014
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Investigation of influence features on expression of lignocellulases in Escherichia coli and selection of bioinformatic tools for prediction of the enzymes expressibility based on amino acid sequences
    World Researchers Associations ; 2022
    In:  Research Journal of Biotechnology Vol. 17, No. 3 ( 2022-02-25), p. 119-128
    Details
    In: Research Journal of Biotechnology, World Researchers Associations, Vol. 17, No. 3 ( 2022-02-25), p. 119-128
    Abstract: Lignocellulases are the most important enzymes for bioeconomy development and have gained many interests in mining new coding-genes from metagenomic DNA data recently. However, the identification of genes suitable for successful expression in E. coli for the enzyme characterization is still a big challenge. In this study, 18 lignocellullase genes from metagenomic data of bacteria in goats' rumen, termite gut and humus were expressed in E. coli. Then 18 nucleotide and amino acid sequences were used to measure 12 impact features and to investigate tools for prediction of their E. coli expressibility. The features closely related to the enzymes expression level included aliphatic side chains of amino acids (aliphatic index: AI), grand average of hydropathicity, protein folding ability (fold index: FI) and FI was the most important factor. The investigation of two models for prediction of the enzymes expressibility in E. coli showed that 100% sequences predicted to be high expressibility by Periscope were the sequences expressed at high level in experiments. In contrast, 100% sequences predicted to be low expressibilty by ESPRESSO constituted a low expression level in experiments. This result can be a good reference for screening genes before expressing in E. coli.
    Type of Medium: Online Resource
    ISSN: 0973-6263 , 2278-4535
    URL: Article
    DOI: 10.25303/1703rjbt119128
    Language: Unknown
    Publisher: World Researchers Associations
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Transplantation of umbilical cord blood-derived mesenchymal stem cells to treat liver cirrhosis in mice: a comparison of tail and portal vein injection
    Biomedical Research and Therapy ; 2017
    In:  Progress in Stem Cell Vol. 4, No. 2 ( 2017-09-10), p. 201-
    Details
    In: Progress in Stem Cell, Biomedical Research and Therapy, Vol. 4, No. 2 ( 2017-09-10), p. 201-
    Abstract: Introduction: To date, there have been many studies indicating the positive effects of stem cells on treating liver cirrhosis. In this study, we used umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) for treatment in a mouse model of liver cirrhosis. Specifically, we determined and compared the effectiveness of two methods of MSC injection (tail vein versus portal vein). Methods: Liver cirrhosis in male Swiss mice (of age approximately 11 weeks or under) was induced by administration of carbon tetrachloride (CCl4; 1 ml/kg). One million UCB-MSCs were then transplanted into cirrhotic mice via the portal vein or tail vein. After 21 days, blood samples were collected for measurement of transaminase, bilirubin and albumin. The expression of fibrosis-associated genes, specifically procollagen – alpha 1 and integrin – beta1, were assessed using quantitative RT-PCR. The histopathology of the specimens was also evaluated using hematoxylin/eosin, Masson trichrome staining, and immunohistochemistry using collagen type 1 and alpha-SMA antibodies. Results: After 21 days, cirrhotic mice treated with UCB-MSCs showed recovery of bilirubin index, increase of liver albumin synthesis, inhibition of fibrosis-related gene expression (e.g. procollagen – alpha 1 and integrin – beta1), and remodeling of liver histology. From comparison of the different routes of transplantation, UCB-portal route was significantly more effective than UCB-tail route at reducing aspartate transaminase (AST) activity and bilirubin index (P 〈 0.05), and inhibiting procollagen – alpha 1 and integrin – beta1 expression (P 〈 0.05). UCB-MSCs from both transfusion routes showed accelerated improvement of liver histopathology. Conclusion: Therapeutic strategies using UCB-MSCs have proven to be promising for the treatment of liver cirrhosis. Injection of UCB-MSC via portal vein was more effective than tail vein for cirrhosis treatment.   Peer Review Details Peer review method: Single-Blind (Peer-reviewers: 02) Peer-review policy Plagiarism software screening?: Yes Date of Original Submission: 17 August 2017 Date accepted: 30 August 2017 Peer reviewers approved by: Dr. Lili Hami Editor who approved publication: Dr. Phuc Van Pham  
    Type of Medium: Online Resource
    ISSN: 2199-4633 , 2199-4633
    URL: Article
    DOI: 10.15419/psc.v4i2.365
    Language: Unknown
    Publisher: Biomedical Research and Therapy
    Publication Date: 2017
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    De Novo Metagenomic Analysis of Microbial Community Contributing in Lignocellulose Degradation in Humus Samples Harvested from Cuc Phuong Tropical Forest in Vietnam
    MDPI AG ; 2022
    In:  Diversity Vol. 14, No. 3 ( 2022-03-17), p. 220-
    Details
    In: Diversity, MDPI AG, Vol. 14, No. 3 ( 2022-03-17), p. 220-
    Abstract: We aimed to investigate the microbial diversity, mine lignocellulose-degrading enzymes/proteins, and analyze the domain structures of the mined enzymes/proteins in humus samples collected from the Cuc Phuong National Park, Vietnam. Using a high-throughput Illumina sequencer, 52 Gbs of microbial DNA were assembled in 2,611,883 contigs, from which 4,104,872 open reading frames (ORFs) were identified. Among the total microbiome analyzed, bacteria occupied 99.69%; the five ubiquitous bacterial phyla included Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, and Acidobacteria, which accounted for 92.59%. Proteobacteria (75.68%), the most dominant, was 5.77 folds higher than the second abundant phylum Bacteroidetes (13.11%). Considering the enzymes/proteins involved in lignocellulose degradation, 22,226 ORFs were obtained from the annotation analysis using a KEGG database. The estimated ratio of Proteobacteria/Bacteroidetes was approximately 1:1 for pretreatment and hemicellulases groups and 2.4:1 for cellulases. Furthermore, analysis of domain structures revealed their diversity in lignocellulose-degrading enzymes. CE and PL were two main families in pretreatment; GH1 and GH3-FN3 were the highest domains in the cellulase group, whereas GH2 and GH43 represented the hemicellulase group. These results validate that natural tropical forest soil could be considered as an important source to explore bacteria and novel enzymes/proteins for the degradation of lignocellulose.
    Type of Medium: Online Resource
    ISSN: 1424-2818
    URL: Article
    DOI: 10.3390/d14030220
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518137-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Expression of eg9 gene encoding endoglucanase GH8 derived from metagenomic DNA data of bacteria in humus around white rot fungi degrading woods, in Escherichia coli
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 4 ( 2022-05-03), p. 645-650
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4 ( 2022-05-03), p. 645-650
    Abstract: Endoglucanase is an important enzyme participating in the hydrolysis of cellulose into oligosaccharides or glucoses can be used in a variety of industrial fields. A gene (code GL0183420 designated as eg9) of 1398 bp encoding for endoglucanase family GH8 was identified from metagenomic DNA data of bacteria in the humus surrounding wood hydrolyzed by white rot fungi. The 126 terminal nucleotides at 5' terminal of the gene encode for a signal peptide in gram-negative bacteria and the next 1269 nucleotides encode the endoglucanase GH8. In this study, the eg9 gene (lack of sequence encoding the signal peptid) coding for the mature endoglucanase was codon optimized for expression in E. coli, artificical synthesized, then inserted into pET22b(+) for gene expression in E. coli strains. Through a survey of 5 expressive strains, EG9 was overexpressed in Rosetta and C43 strains. However, at 30oC in LB medium, most of EG9 were expressed in the insoluble fraction, in which Rosetta was the best strain for synthesis of EG9. The enzyme EG9 can be overexpressed in Rosetta strain at a large range of OD600 from 0.2 to 1. After reducing the culture temperature to 25°C, 50% of EG9 was expressed in soluble fraction and exhibited good hydrolysis activity in CMC substrate on agar plate. The investigation of media showed that PE was the most suitable medium for the recombinant Rosetta strain grow and synthesize endoglucanase EG9. EG9 was well expressed at various IPTG concentrations from 0.05 to 0.9 mM. These results offer great potential for production of recombinant EG9 for enzyme properties research.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15869
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    ID: 1055 Adipose-derived stem cells and platelet rich plasma ameliorate liver cirrhotic circumstance in CCl4-induced mice: a new approach for liver cirrhosis treatment
    Biomedical Research and Therapy ; 2017
    In:  Biomedical Research and Therapy Vol. 4, No. S ( 2017-09-05), p. 136-
    Details
    In: Biomedical Research and Therapy, Biomedical Research and Therapy, Vol. 4, No. S ( 2017-09-05), p. 136-
    Abstract: Background: Stem cell therapy in liver cirrhosis treatment is attracting the attention of the scientific community. Adipose tissue-derived mesenchymal stem cells are a potential source of cells because they have self-renewal, high proliferation, and differentiation into a variety of cell types, including hepatocytes as potential cell sources for cirrhosis treatment. Platelet-rich plasma (PRP) growth factors contribute to regeneration and wound healing. We test the hypothesis that PRP co-administration enhances MSC treatment for mouse cirrhosis. Method: Male Swiss mice were treated orally with olive oil or CCl4 for 11 weeks. PRP was obtained from healthy mice. Mouse adipose-derived stem cells (mADSCs) from adipose tissue of 3 weeks CCl4 mice were cultured for three passages (P3-mADSCs) before the transfer by tail vein injection with or without PRP into 11 weeks CCl4 mice. Mice were divided into six groups (n=10 each group). 1) normal, 2) cirrhotic, 3) cirrhotic /PBS; 4) cirrhotic/PRP (0.2 ml/mice with PRP from healthy mice), 5) cirrhotic/mADSCs (5 x 105 cells/mice), and 6) cirrhotic/mADSC-PRP.   Result: mADSCs were highly positive for CD44, CD90, and CD105. Relative to liver cells, P3-mADSCs highly expressed Alb, Ck18, Ck19, Tnf, c-met, Cyp1a1, Afp, Muc1, Ldl receptor. mADSCs were strongly positive for Cyp1a1 (98.21±1.57%) and Hgf (95.55±3.11%); moderately positive for alfa-fetoprotein (45.99±2.08%), Aat (44.43±7.79%), Alb (57.81±8.49%) and differentiated into hepatocyte-like cells under induction medium. After transplantation, CFDA-transplanted cells into CCl4-treated mice were found in the liver at day 21 st. Compared to mADSCs, mADSCs and PRP co-treatment most effectively improved serum AST/ALT/bilirubin and albumin levels in day seven cirrhotic mice (p 〈 0.05); and significantly down-regulated procollagen (104-fold less) and TGF-beta 1 (10-fold less) in day 21 cirrhotic liver. Histology index and collagen deposition were improved in 100% of mADSC/PRP- and mADSCs- cirrhotic mice compared to 33.3% of PBS- or PRP- cirrhotic liver (p 〈 0.05). Conclusion: Cultured mADSCs express hepatocyte enriched markers. PRP coadministration enhances mADSC effects to improve liver function further, and further reduce fibrosis.
    Type of Medium: Online Resource
    ISSN: 2198-4093 , 2198-4093
    URL: Article
    DOI: 10.15419/bmrat.v4iS.330
    Language: Unknown
    Publisher: Biomedical Research and Therapy
    Publication Date: 2017
    detail.hit.zdb_id: 2806789-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Some characters of bacterial cellulases in goats' rumen elucidated by metagenomic DNA analysis and the role of fibronectin 3 module for endoglucanase function
    Asian Australasian Association of Animal Production Societies ; 2021
    In:  Animal Bioscience Vol. 34, No. 5 ( 2021-05-01), p. 867-879
    Details
    In: Animal Bioscience, Asian Australasian Association of Animal Production Societies, Vol. 34, No. 5 ( 2021-05-01), p. 867-879
    Abstract: Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined.Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation.Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis.Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.
    Type of Medium: Online Resource
    ISSN: 2765-0189 , 2765-0235
    URL: Article
    DOI: 10.5713/ajas.20.0115
    Language: English
    Publisher: Asian Australasian Association of Animal Production Societies
    Publication Date: 2021
    detail.hit.zdb_id: 3055169-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Comparative treatment efficiency of adipose and bone marrow derived allogenic mesenchymal stem cell transplantation in mouse models of liver fibrosis
    Biomedical Research and Therapy ; 2017
    In:  Biomedical Research and Therapy Vol. 4, No. 06 ( 2017-06-25), p. 1374-
    Details
    In: Biomedical Research and Therapy, Biomedical Research and Therapy, Vol. 4, No. 06 ( 2017-06-25), p. 1374-
    Abstract: Background: The application of mesenchymal stem cell (MSC) therapy in liver fibrosis treatment has been increasingly investigated in recent years. MSCs obtained from a variety of sources (e.g. bone marrow, umbilical cord blood and adipose tissue) have been studied and have achieved remarkable results. In this study, we compared the effects of adipose-derived mesenchymal stem cells (AD-MSC) transplantation with bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in a mouse model of liver fibrosis, induced by carbon tetrachloride (CCl4). Methods: Eight-week old mice were treated with CCl4 for 11 weeks to induce liver fibrosis then 5x105 cells were transplanted into mice via the tail vein. Results: After 21 days of transplantation, the results showed that the stem cell treated groups ameliorated better than the placebo group. MSC treated groups showed reduced AST and ALT levels, down-regulated expression of extracellular matrix (ECM) genes, and improved liver histopathology. Both sources of MSCs (bone marrow and adipose tissue) were effective in the mouse model of liver fibrosis. Conclusion: Our results also indicated that AD-MSC transplantation in mice accelerated liver regeneration better than BM-MSC transplantation.
    Type of Medium: Online Resource
    ISSN: 2198-4093 , 2198-4093
    URL: Article
    DOI: 10.15419/bmrat.v4i06.179
    Language: Unknown
    Publisher: Biomedical Research and Therapy
    Publication Date: 2017
    detail.hit.zdb_id: 2806789-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    ID: 1074 The efficiency of adipose-derived stem cell pretreated with hepatocyte growth factor and platelet rich plasma on a liver cirrhosis mouse model
    Biomedical Research and Therapy ; 2017
    In:  Biomedical Research and Therapy Vol. 4, No. S ( 2017-09-05), p. 157-
    Details
    In: Biomedical Research and Therapy, Biomedical Research and Therapy, Vol. 4, No. S ( 2017-09-05), p. 157-
    Abstract: Background: Because of their ease of isolation, high proliferation capacity in vitro, as well as their ability to differentiate into liver cells, Adipose-derived stem cell (ADSCs), are considered to be a promised candidate for liver disease treatment, including liver cirrhosis treatment. Recent studies show that hepatocyte growth factor (HGF) can stimulate ADSC to migrate to the injured areas and platelet rich plasma (PRP) can increase the stemness of ADSCs. Method: In this study, we cultured ADSCs in the medium supplemented 20 ng/ml and 10% PRP; then transplanted them into the cirrhosis mouse model. After 11 weeks of CCl4 induction (1ml/kg, three times per week), mice were divided into 3 groups: (1) PBS group which were injected 0.2 ml PBS; (2) ADSC group which were transplanted 5x105 ADSCs non-pretreated with HGF and PRP; (3) ADSC/HGF+PRP group which were transplanted 5x105 ADSCs pretreated with HGF and PRP via the tail vein. We evaluated the effectiveness of the therapeutic at the day 7th and 14th after transplantation. Results: The results show that the transplantation of ADSC pretreated with HGF and PRP after seven days improves the body weight (increase 4.673%); decreases the ALT level (p 〈 0.05), the total leukocyte number (p 〈 0.05) and the expression of Pro-collagen (decrease 4.1 times) as well as α-SMA (decrease 5.1 times), in comparison to the ADSC group. ADSCs pretreated with HGF and PRP also help to improve the liver tissue structure. Conclusion: The therapy using ADSCs pretreated with HGF and PRP is considered to be a promising therapy.
    Type of Medium: Online Resource
    ISSN: 2198-4093 , 2198-4093
    URL: Article
    DOI: 10.15419/bmrat.v4iS.348
    Language: Unknown
    Publisher: Biomedical Research and Therapy
    Publication Date: 2017
    detail.hit.zdb_id: 2806789-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    ID: 1035 Transplantation of umbilical cord blood-derived mesenchymal stem cells to treat liver cirrhosis in mice: a comparison of tail and portal vein injection
    Biomedical Research and Therapy ; 2017
    In:  Biomedical Research and Therapy Vol. 4, No. S ( 2017-09-05), p. 111-
    Details
    In: Biomedical Research and Therapy, Biomedical Research and Therapy, Vol. 4, No. S ( 2017-09-05), p. 111-
    Abstract: Background: Up to date, there have been some studies indicating positive effects of stem cells on treating the liver cirrhosis. In this study, we compared the effectiveness of two methods in which mesenchymal stem cells harvested from umbilical cord blood (UCB-MSCs) were transfused either via portal or tail veins to the mouse models of liver cirrhosis.  Methods: Liver cirrhosis was induced by CCl4 (1 ml/kg) on male Swiss mice within 11 weeks, followed by administration of 106 UCB-MSCs via the portal or tail vein. After 21 days, blood samples were collected for measuring transaminase, bilirubin and albumin activities. The expression of fibrosis-associated genes, specifically procollagen – alpha 1 and integrin – beta1, were assessed using qRT-PCR. The histopathology was also evaluated using hematoxylin/eosin, Masson trichrome staining and immunohistochemistry with collagen type 1 and alpha-SMA antibody.  Results: UCB-MSCs transplantation significantly improved post-21 days of treatment in the liver fibrosis mice as compared with placebo group. Notably, UCB-MSCs transferred through portal veins revealed a more positive effect than via tail veins as indicated by the improvement in the biochemical indexes, fibrosis-related genes expression, and liver histopathology.  Conclusion: The UCB-MSCs therapy proved to be a promising method for treating the liver cirrhosis. The method of delivering stem cells through portal vein was more effective than through tail vein
    Type of Medium: Online Resource
    ISSN: 2198-4093 , 2198-4093
    URL: Article
    DOI: 10.15419/bmrat.v4iS.311
    Language: Unknown
    Publisher: Biomedical Research and Therapy
    Publication Date: 2017
    detail.hit.zdb_id: 2806789-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...