In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 72, No. 8_Supplement ( 2012-04-15), p. LB-116-LB-116
Abstract:
Currently, SCID mice are the major biomedical model for the study of human cancer and other diseases; however, they can be a poor model because there is a large discrepancy in body size and physiology between rodents and humans. Since most new anticancer drugs are first evaluated in rodents, most fail in phase I and phase II clinical trials. Thus, there is a tremendous demand for more sophisticated animal models, which may improve the translation efficiency from preclinical to clinical studies. We recently identified pigs that are severely immunocompromised. Here we tested these pigs as a model for human tumors. Three immunodeficient (ID) pigs and three normal (WT) pigs, six weeks of age, were tested for their ability to allow xenotransplanted human tumors to grow. Each pig was transplanted with 4 million A375 human malignant melanoma cells subcutaneously into the left ear and 4 million PANC-1 human pancreatic carcinoma cells subcutaneously into the right ear on day 0. The ID pigs were euthanized 6, 14, or 23 days after cell transplantation, based on their health condition. Palpable melanoma tumors were identified on day 13 after cell transplantation in the two remaining ID pigs. After euthanasia, full necropsy was performed on all pigs; tissues were collected from ears and other organs for histopathological analysis. Melanoma and pancreatic tumor xenotransplants were identified histologically in all three ID pigs. The presence of human cancer cells in these pigs was further verified with anti-human mitochondrial immunohistochemistry. No transplanted tumors were found, grossly, histologically, or immunohistochemically, in the WT pigs. We propose that this genetic line of ID pigs may prove to be a useful large animal model for human tumors. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-116. doi:1538-7445.AM2012-LB-116
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2012-LB-116
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2012
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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