In:
American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 64, No. 4 ( 2003-04-01), p. 396-403
Abstract:
Objective —To isolate bovine mammary gland cells
with stem cell characteristics. Sample Population —Monolayers of bovine mammary gland cells. Procedure —Mammary gland cell populations were
separated by use of selected media supplements. Phenotypic characteristics were examined via light
and transmission electron microscopy. Cellular expression of casein and connexin 43 was identified
immunohistochemically. A scrape-loading and dye transfer assay was used to examine the mammary
gland cell populations for homogenous gap junctional intercellular communication (GJIC). Results —Subpopulations of mammary gland cells
grown in vitro are classified on the basis of their distinct morphologic features and ability to communicate via gap
junctions. Ultrastructurally, 2 morphologically distinct cell types were classified as type I and II cells. Type I cells
were small light undiffertiated cells and large light undifferentiated cells that were deficient in functional gap
junctions (as is characteristic of stem cells). Type II cells included large light differentiated cells and terminally differentiated
cells; GJIC was functional in type II cells. Type II cells had cytoplasmic expression of connexin 43,
whereas, type I cells did not. All cells expressed casein. Conclusions and Clinical Relevance —Subpopulations
of bovine mammary gland cells with stem cell characteristics were identified. Phenotypic differences
are observed among type I bovine mammary gland cells with stem cell characteristics. Gap junctional
intercellular communication may be necessary for the differentiation of stem cells. Characterization
of bovine mammary gland stem cells and their progeny may provide a new tool with which to study mammary
gland health. ( Am J Vet Res 2003;63:396–403)
Type of Medium:
Online Resource
ISSN:
0002-9645
DOI:
10.2460/ajvr.2003.64.396
Language:
Unknown
Publisher:
American Veterinary Medical Association (AVMA)
Publication Date:
2003
detail.hit.zdb_id:
2056942-7
SSG:
22
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