In:
Limnology and Oceanography, Wiley, Vol. 37, No. 7 ( 1992-11), p. 1434-1446
Abstract:
Unialgal cultures were used to investigate relationships between cell volume and the carbon and nitrogen content of nondiatomaceous marine nanophytoplankton. Cell dimensions were determined by image‐analyzed epifluorescence microscopy and particulate C and N by high‐temperature dry combustion. Volumes were calculated by direct integration with published algorithms (biovolume), but could be estimated equally well from linear dimensions as prolate spheres. Preservation with 0.5% glutaraldehyde reduced cell volumes 29% on average. Correlations were highly significant between biovolume of preserved cells and C and N contents. Nonlinear regression models appeared most appropriate because smaller cells contained more C and N per unit volume than did larger cells. Suggested general C densities for estimating cell C from preserved volume were 0.36 pg µ m −3 for 10 1 µ m 3 cells, 0.24 pg µ m −3 for 10 2 µ m 3 cells, and 0.16 pg C µ m −3 for 10 3 µm 3 cells. Previous regression models substantially underestimated the C densities of nanophytoplankton of 10 1 –10 3 µ m 3 . The explanation for these differences includes the method of determining mean population volumes, the use of preservatives, and the occurrence of significant vacuolar volume in larger phytoplankton.
Type of Medium:
Online Resource
ISSN:
0024-3590
,
1939-5590
DOI:
10.4319/lo.1992.37.7.1434
Language:
English
Publisher:
Wiley
Publication Date:
1992
detail.hit.zdb_id:
2033191-5
detail.hit.zdb_id:
412737-7
SSG:
12
SSG:
14
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