In:
Frontiers in Cell and Developmental Biology, Frontiers Media SA, Vol. 9 ( 2021-3-19)
Abstract:
Cell fate decisions during development are governed by multi-factorial regulatory mechanisms including chromatin remodeling, DNA methylation, binding of transcription factors to specific loci, RNA transcription and protein synthesis. However, the mechanisms by which such regulatory “dimensions” coordinate cell fate decisions are currently poorly understood. Here we quantified the multi-dimensional molecular changes that occur in mouse embryonic stem cells (mESCs) upon depletion of Estrogen related receptor beta (Esrrb), a key pluripotency regulator. Comparative analyses of expression changes subsequent to depletion of Esrrb or Nanog, indicated that a system of interlocked feed-forward loops involving both factors, plays a central part in regulating the timing of mESC fate decisions. Taken together, our meta-analyses support a hierarchical model in which pluripotency is maintained by an Oct4-Sox2 regulatory module, while the timing of differentiation is regulated by a Nanog-Esrrb module.
Type of Medium:
Online Resource
ISSN:
2296-634X
DOI:
10.3389/fcell.2021.630067
DOI:
10.3389/fcell.2021.630067.s001
DOI:
10.3389/fcell.2021.630067.s002
DOI:
10.3389/fcell.2021.630067.s003
DOI:
10.3389/fcell.2021.630067.s004
DOI:
10.3389/fcell.2021.630067.s005
DOI:
10.3389/fcell.2021.630067.s006
DOI:
10.3389/fcell.2021.630067.s007
DOI:
10.3389/fcell.2021.630067.s008
DOI:
10.3389/fcell.2021.630067.s009
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2737824-X
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