In:
The Journal of Immunology, The American Association of Immunologists, Vol. 120, No. 5 ( 1978-05-01), p. 1782-1783
Abstract:
Fetal bovine serum is in widespread use for in vitro antibody-synthesizing systems, but very little attention has been paid to the complement (C) activity of this serum, at least partly because methods have not been developed for measuring some bovine C components, particularly C3. We report here some studies on bovine C1, and a simple assay for bovine C3 and its use to measure the C3 content of a number of commercial and other fetal bovine serum preparations, which were “good” or “bad” with respect to their ability to support (in vitro antibody synthesis. The C1, C3b-inactivator and conglutinin contents of these preparations will also be reported. C1 was precipitated twice from normal bovine serum at 0.03 M NaCl and 0.005 M phosphate concentration, pH 7.5 (O. Barta, R. A. Nelson, C. Y. Kuo, Immunol. Commun. 5:75, 1976). It had a C1H50 titer of 210,000 [vs 33,000 for Cordis guinea pig (GP) C1] in a standard assay with EAC4gp, yielding a Z plot slope of 1.00.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.120.5.1782.c
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1978
detail.hit.zdb_id:
1475085-5
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