In:
Science and Technology Development Journal, Viet Nam National University Ho Chi Minh City, Vol. 14, No. 3 ( 2011-09-30), p. 54-63
Abstract:
hG-CSF is a cytokine that stimulates the proliferation, differentiation, function of mature neutrophils and is generally used for treatment of neutropenia in cancer patients under chemotherapy or other diseased patients. After production process, E. coli-derived recombinant hGCSF is usually available as non-glycosylated, liquid protein, so that it’s often unstable, easy to lose their biological activity. In this study, we report the results of using lyophilization to storage recombinant hG-CSF. We found out the freezing conditions and the suitable protein volumes for freezedrying process. Furthermore, we also studied the effects of lyophilization on this protein by testing the structure with Native-PAGE, RP-HPLC, testing biological activity on M-NFS-60 cell lines and in mouse (Mus musculus).
Type of Medium:
Online Resource
ISSN:
1859-0128
DOI:
10.32508/stdj.v14i3.1989
Language:
Unknown
Publisher:
Viet Nam National University Ho Chi Minh City
Publication Date:
2011
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