In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 54, No. 6 ( 2004-11-01), p. 2185-2190
Abstract:
The taxonomic position of a halo- and organo-sensitive, oligotrophic soil bacterium, strain S213 T , was investigated. Cells were Gram-negative, non-motile, strictly aerobic, yellow-pigmented rods of short to medium length on diluted nutrient broth. When 0·1–0·4 % (w/v) NaCl was added to diluted media composed of peptone and meat extract, growth was inhibited with increasing NaCl concentration and the cells became long aberrant forms. When 6 mM CaCl 2 was added, the cells grew quite normally and aberrant cells were no longer found at 0·1–0·5 % (w/v) NaCl. Chemotaxonomically, strain S213 T contains chemical markers that indicate its assignment to the Sphingomonadaceae : the presence of ubiquinone Q-10 as the predominant respiratory quinone, C 18 : 1 and C 16 : 0 as major fatty acids, C 14 : 0 2-OH as the major 2-hydroxy fatty acid and sphingoglycolipids. 16S rRNA gene sequence analysis indicated that strain S213 T belongs to the genus Sphingomonas , exhibiting high sequence similarity to the 16S rRNA gene sequences of Sphingomonas mali IFO 15500 T (98·3 %), Sphingomonas pruni IFO 15498 T (98·0 %), Sphingomonas asaccharolytica IFO 15499 T (97·9 %) and Sphingomonas echinoides DSM 1805 T (97·8 %). The results of DNA–DNA hybridization experiments and its phenotypic characteristics clearly distinguished the strain from its nearest neighbours and demonstrate that strain S213 T represents a novel Sphingomonas species, for which the name Sphingomonas oligophenolica sp. nov. is proposed. The type strain is S213 T (=JCM 12082 T =CIP 107926 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.02959-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2004
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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