In:
PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 12 ( 2020-12-3), p. e0243272-
Abstract:
Cluster of differentiation (CD) 166 or activated leukocyte cell adhesion molecule (ALCAM) is a transmembrane molecule known to be an intercellular adhesion factor. The expression and function of ALCAM in medulloblastoma (MB), a pediatric brain tumor with highly advanced molecular genetics, remains unclear. Therefore, this study aimed to clarify the significance and functional role of ALCAM expression in MB. ALCAM expression in 45 patients with MB was evaluated by immunohistochemical analysis of formalin-fixed paraffin-embedded clinical specimens and the relationship between ALCAM expression and pathological type/molecular subgroup, such as WNT, SHH, Group 3, and Group 4, was examined. Eight ALCAM positive (18%), seven partially positive (16%), and 30 negative (67%) cases were detected. All seven cases of the WNT molecular subgroup were ALCAM positive and ALCAM expression strongly correlated with this subgroup ( P 〈 0.0001). In addition, functional studies using MB cell lines revealed ALCAM expression affected proliferation and migration as a positive regulator in vitro . However, ALCAM silencing did not affect survival or the formation of leptomeningeal dissemination in an orthotopic mouse model, but did induce a malignant phenotype with increased tumor cell invasion at the dissemination sites (P = 0.0029). In conclusion, our results revealed that ALCAM exhibited highly specific expression in the WNT subgroup of MB. Furthermore, we demonstrated that the cell kinetics of MB cell lines can be altered by the expression of ALCAM.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0243272
DOI:
10.1371/journal.pone.0243272.g001
DOI:
10.1371/journal.pone.0243272.g002
DOI:
10.1371/journal.pone.0243272.g003
DOI:
10.1371/journal.pone.0243272.g004
DOI:
10.1371/journal.pone.0243272.t001
DOI:
10.1371/journal.pone.0243272.t002
DOI:
10.1371/journal.pone.0243272.s001
DOI:
10.1371/journal.pone.0243272.s002
DOI:
10.1371/journal.pone.0243272.s003
DOI:
10.1371/journal.pone.0243272.s004
DOI:
10.1371/journal.pone.0243272.s005
DOI:
10.1371/journal.pone.0243272.s006
DOI:
10.1371/journal.pone.0243272.r001
DOI:
10.1371/journal.pone.0243272.r002
DOI:
10.1371/journal.pone.0243272.r003
DOI:
10.1371/journal.pone.0243272.r004
DOI:
10.1371/journal.pone.0243272.r005
DOI:
10.1371/journal.pone.0243272.r006
DOI:
10.1371/journal.pone.0243272.r007
DOI:
10.1371/journal.pone.0243272.r008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2267670-3
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