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  • 1
    Online Resource
    Online Resource
    Evaluation of Antioxidant, Antimicrobial, and Cytotoxic Activities and Correlation with Phytoconstituents in Some Medicinal Plants of Nepal
    Hindawi Limited ; 2022
    In:  Journal of Chemistry Vol. 2022 ( 2022-11-18), p. 1-10
    Details
    In: Journal of Chemistry, Hindawi Limited, Vol. 2022 ( 2022-11-18), p. 1-10
    Abstract: Traditional herbal medicines have been consumed in Nepal and other parts of the eastern hemisphere since ancient times. Many of these plants reportedly have been effective against ailments as well. This study aims to analyze the phytochemical constituents from the extracts of ten such plants and evaluate their antimicrobial, cytotoxicity, and antioxidant properties. In addition, the study aims to study the correlation of cytotoxicity and antioxidant activities with the total phenolic, flavonoid, and tannin contents. The plants investigated were Oroxylum indicum, Kalanchoe pinnata, Phragmites vallatoria, Ehretia acuminata, Cirsium wallichii, Ampelocissus tomentosa, Dichrocephala integrifolia, Boenninghausenia albiflora, Cynoglossum zeylanicum, and Clerodendrum serratum. Phytochemical analyses were performed to evaluate secondary metabolites, such as glycosides, flavonoids, terpenoids, saponins, alkaloids, and fats. The total phenolic contents of the extracts ranged from 14.94 to 229.89 mg GAE/g, the total flavonoid contents varied from 66.67 to 900 mg QE/g, and the total tannin contents were 42 to 168 mg GAE/g. The results of the antioxidant studies showed that the highest antioxidant activity was exhibited by the extract of A. tomentosa (IC50 = 7.89 µg/mL) followed by E. acuminata (IC50 = 24.82 µg/mL) and C. serratum (IC50 = 32.91 µg/mL). The extracts from P. vallatoria and A. tomentosa exhibited substantial antimicrobial activity. The extracts of A. tomentosa and B. albiflora showed lethality against brine shrimp with LC50 values of 33.11 µg/mL.
    Type of Medium: Online Resource
    ISSN: 2090-9071 , 2090-9063
    URL: Article
    DOI: 10.1155/2022/4725801
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2393625-3
    detail.hit.zdb_id: 2703077-5
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  • 2
    Online Resource
    Online Resource
    Metabolome Mining of Curcuma longa L. Using HPLC-MS/MS and Molecular Networking
    MDPI AG ; 2023
    In:  Metabolites Vol. 13, No. 8 ( 2023-07-29), p. 898-
    Details
    In: Metabolites, MDPI AG, Vol. 13, No. 8 ( 2023-07-29), p. 898-
    Abstract: Turmeric, Curcuma longa L., is a type of medicinal plant characterized by its perennial nature and rhizomatous growth. It is a member of the Zingiberaceae family and is distributed across the world’s tropical and subtropical climates, especially in South Asia. Its rhizomes have been highly valued for food supplements, spices, flavoring agents, and yellow dye in South Asia since ancient times. It exhibits a diverse array of therapeutic qualities that encompass its ability to combat diabetes, reduce inflammation, act as an antioxidant, exhibit anticancer properties, and promote anti-aging effects. In this study, organic extracts of C. longa rhizomes were subjected to HPLC separation followed by ESI-MS and low-energy tandem mass spectrometry analyses. The Global Natural Product Social Molecular Networking (GNPS) approach was utilized for the first time in this ethnobotanically important species to conduct an in-depth analysis of its metabolomes based on their fragments. To sum it up, a total of 30 metabolites including 16 diarylheptanoids, 1 diarylpentanoid, 3 bisabolocurcumin ethers, 4 sesquiterpenoids, 4 cinnamic acid derivatives, and 2 fatty acid derivatives were identified. Among the 16 diarylheptanoids identified in this study, 5 of them are reported for the first time in this species.
    Type of Medium: Online Resource
    ISSN: 2218-1989
    URL: Article
    DOI: 10.3390/metabo13080898
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662251-8
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  • 3
    Online Resource
    Online Resource
    Assessment of the Role of Leasehold Forest in Livelihood Improvement in Gorkha District, Nepal
    Agricultural & Environmental Technology Development Society ; 2023
    In:  International Journal of Agricultural and Applied Sciences Vol. 4, No. 1 ( 2023-06-30), p. 120-126
    Details
    In: International Journal of Agricultural and Applied Sciences, Agricultural & Environmental Technology Development Society, Vol. 4, No. 1 ( 2023-06-30), p. 120-126
    Abstract: The leasehold forestry aims to free the poor from a complicated cycle of poverty by institutionalizing and manipulating the local resources. This study is based on the structured and semi-structured questionnaire and an examination of both published and unpublished records. The study was carried out in 283 Leasehold Forest User Groups (LHFUGs) of the Gorkha district representing 8 different clusters. This study makes a clear effort to identify livelihood improvement in the Gorkha district through leasehold forest by taking into consideration livelihood assets (natural, physical, human, financial, and social). The condition of livelihood capitals in the study area was examined using a judgmental scoring technique that looked at the changes that occurred in five different capitals. Three indicators have been used for each of the assets and scores; +1, -1, and 0 were given to represent improved, degraded, and remained unchanged respectively. A spider web diagram was used to express the change. The results depicted that Chepang, Gurung, and Magar are the predominant indigenous peoples who benefited from the LHF. The result showed the constructive impact of the leasehold forestry program on each asset. The social, physical, human, natural and financial capital received an average total score of 0.778, 0.951, 0.748, 0.589, and 0.722 respectively. The most notable accomplishments were construction and access to the physical capitals including the effectiveness of constructions and advancement of knowledge about community development. Better-managed natural resources can help achieve both conservation and livelihood options.
    Type of Medium: Online Resource
    ISSN: 2582-8053
    URL: Issue
    URL: Article
    DOI: 10.52804/ijaas2023
    DOI: 10.52804/ijaas2023.4120
    Language: Unknown
    Publisher: Agricultural & Environmental Technology Development Society
    Publication Date: 2023
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  • 4
    Online Resource
    Online Resource
    Technological Advancements for the Detection of Antibiotics in Food Products
    MDPI AG ; 2021
    In:  Processes Vol. 9, No. 9 ( 2021-08-25), p. 1500-
    Details
    In: Processes, MDPI AG, Vol. 9, No. 9 ( 2021-08-25), p. 1500-
    Abstract: Antibiotics, nowadays, are not only used for the treatment of human diseases but also used in animal and poultry farming to increase production. Overuse of antibiotics leads to their circulation in the food chain due to unmanaged discharge. These circulating antibiotics and their residues are a major cause of antimicrobial resistance (AMR), so comprehensive and multifaceted measures aligning with the One Health approach are crucial to curb the emergence and dissemination of antibiotic resistance through the food chain. Different chromatographic techniques and capillary electrophoresis (CE) are being widely used for the separation and detection of antibiotics and their residues from food samples. However, the matrix present in food samples interferes with the proper detection of the antibiotics, which are present in trace concentrations. This review is focused on the scientific literature published in the last decade devoted to the detection of antibiotics in food products. Various extraction methods are employed for the enrichment of antibiotics from a wide variety of food samples; however, solid-phase extraction (SPE) techniques are often used for the extraction of antibiotics from food products and biological samples. In addition, this review has scrutinized how changing instrumental composition, organization, and working parameters in the chromatography and CE can greatly impact the identification and quantification of antibiotic residues. This review also summarized recent advancements in other detection methods such as immunological assays, surface-enhanced Raman spectroscopy (SERS)-based assays, and biosensors which have emerged as rapid, sensitive, and selective tools for accurate detection and quantification of traces of antibiotics.
    Type of Medium: Online Resource
    ISSN: 2227-9717
    URL: Article
    DOI: 10.3390/pr9091500
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2720994-5
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