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  • 1
    Online Resource
    Online Resource
    Image_4.JPEG
    Frontiers Media SA ; 2022
    In:  Frontiers in Microbiology Vol. 13 ( 2022-10-13)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-10-13)
    Abstract: Tobacco contains a large amount of bioactive ingredients which can be used as source of feed. The objective of this study was to evaluate the effects of dietary addition of low-nicotine tobacco (LNT) on the growth performance, blood status, cecum microbiota and metabolite composition of meat rabbits. A total of 80 Kangda meat rabbits of similar weight were assigned randomly as four groups, and three of them were supplemented with 5%, 10%, and 20% LNT, respectively, with the other one fed with basal diet as control group. Each experiment group with 20 rabbits was raised in a single cage. The experiments lasted for 40 days with a predictive period of 7 days. The results revealed that LNT supplementation had no significant effect on the growth performance, but increased the half carcass weight compared with control group. Dietary supplemention of LNT decreased the triglycerides and cholesterol content in rabbit serum, and significantly increased the plasma concentration of lymphocytes (LYM), monocytes, eosinophils, hemoglobin HGB and red blood cells. In addition, LNT supplementation significantly changed the microbial diversity and richness, and metagenomic analysis showed that LNT supplementation significantly increased Eubacterium_siraeum_group , Alistipes , Monoglobus and Marvinbryantia at genus level. Moreover, LC–MS data analysis identified a total of 308 metabolites that markedly differed after LNT addition, with 190 significantly upregulated metabolites and 118 significantly downregulated metabolites. Furthermore, the correlation analysis showed that there was a significant correlation between the microbial difference and the rabbit growth performance. Overall, these findings provide theoretical basis and data support for the application of LNT in rabbits.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2022.1026680
    DOI: 10.3389/fmicb.2022.1026680.s001
    DOI: 10.3389/fmicb.2022.1026680.s002
    DOI: 10.3389/fmicb.2022.1026680.s003
    DOI: 10.3389/fmicb.2022.1026680.s004
    DOI: 10.3389/fmicb.2022.1026680.s005
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2587354-4
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  • 2
    Online Resource
    Online Resource
    NtMYB305a binds to the jasmonate-responsive GAG region of NtPMT1a promoter to regulate nicotine biosynthesis
    Oxford University Press (OUP) ; 2022
    In:  Plant Physiology Vol. 188, No. 1 ( 2022-01-20), p. 151-166
    Details
    In: Plant Physiology, Oxford University Press (OUP), Vol. 188, No. 1 ( 2022-01-20), p. 151-166
    Abstract: MYB transcription factors play essential roles in regulating plant secondary metabolism and jasmonate (JA) signaling. Putrescine N-methyltransferase is a key JA-regulated step in the biosynthesis of nicotine, an alkaloidal compound highly accumulated in Nicotiana spp. Here we report the identification of NtMYB305a in tobacco (Nicotiana tabacum) as a regulatory component of nicotine biosynthesis and demonstrate that it binds to the JA-responsive GAG region, which comprises a G-box, an AT-rich motif, and a GCC-box-like element, in the NtPMT1a promoter. Yeast one-hybrid analysis, electrophoretic mobility shift assay and chromatin immunoprecipitation assays showed that NtMYB305a binds to the GAG region in vitro and in vivo. Binding specifically occurs at the ∼30-bp AT-rich motif in a G/C-base-independent manner, thus defining the AT-rich motif as previously unknown MYB-binding element. NtMYB305a localized in the nucleus of tobacco cells where it is capable of activating the expression of a 4×GAG-driven GUS reporter in an AT-rich motif-dependent manner. NtMYB305a positively regulates nicotine biosynthesis and the expression of NtPMT and other nicotine pathway genes. NtMYB305a acts synergistically with NtMYC2a to regulate nicotine biosynthesis, but no interaction between these two proteins was detected. This identification of NtMYB305a provides insights into the regulation of nicotine biosynthesis and extends the roles played by MYB transcription factors in plant secondary metabolism.
    Type of Medium: Online Resource
    ISSN: 0032-0889 , 1532-2548
    URL: Article
    DOI: 10.1093/plphys/kiab458
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
    SSG: 12
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    Online Resource
  • 3
    Online Resource
    Online Resource
    bHLH Transcription Factor NtMYC2a Regulates Carbohydrate Metabolism during the Pollen Development of Tobacco (Nicotiana tabacum L. cv. TN90)
    MDPI AG ; 2021
    In:  Plants Vol. 11, No. 1 ( 2021-12-22), p. 17-
    Details
    In: Plants, MDPI AG, Vol. 11, No. 1 ( 2021-12-22), p. 17-
    Abstract: Basic helix-loop-helix (bHLH) transcription factor MYC2 regulates plant growth and development in many aspects through the jasmonic acid (JA) signaling pathway, while the role of MYC2 in plant carbohydrate metabolism has not been reported. Here, we generated NtMYC2a-overexpressing (NtMYC2a-OE) and RNA-interference-mediated knockdown (NtMYC2a-RI) transgenic plants of tobacco (Nicotiana tabacum L. cv. TN90) to investigate the role of NtMYC2a in carbohydrate metabolism and pollen development. Results showed that NtMYC2a regulates the starch accumulation and the starch-sugar conversion of floral organs, especially in pollen. The RT-qPCR analysis showed that the expression of starch-metabolic-related genes, AGPs, SS2 and BAM1, were regulated by NtMYC2a in the pollen grain, anther wall and ovary of tobacco plants. The process of pollen maturation was accelerated in NtMYC2a-OE plants and was delayed in NtMYC2a-RI plants, but the manipulation of NtMYC2a expression did not abolish the pollen fertility of the transgenic plants. Intriguingly, overexpression of NtMYC2a also enhanced the soluble carbohydrate accumulation in tobacco ovaries. Overall, our results demonstrated that the bHLH transcription factor NtMYC2a plays an important role in regulating the carbohydrate metabolism during pollen maturation in tobacco.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    URL: Article
    DOI: 10.3390/plants11010017
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2704341-1
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  • 4
    Online Resource
    Online Resource
    Manipulation of CBTS1 Expression Alters Tobacco Resistance to Spodoptera frugiperda and Phytophthora nicotianae
    MDPI AG ; 2023
    In:  Agronomy Vol. 13, No. 3 ( 2023-03-14), p. 845-
    Details
    In: Agronomy, MDPI AG, Vol. 13, No. 3 ( 2023-03-14), p. 845-
    Abstract: Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing CBTS1 expression on tobacco resistance to the insect pest Spodoptera frugiperda and oomycete pathogen Phytophthora nicotianae. The CDS sequence of CBTS1 was cloned into gene overexpression and silencing vectors and introduced into tobacco (Nicotiana tabacum L. cv. TN90) to obtain CBTS1-overexpression plants (CBTS1-OE) and CBTS1-silenced plants (CBTS1-RI). Compared with control plants, the content of cembratrien-ol (CBT-ol) was increased 4.48 times in the CBTS1-OE plants but decreased by 68% in the CBTS1-RI plants, while that of cembratrien-diol (CBT-diol) was increased 3.17 times in the CBTS1-OE plants but decreased by 76% in the CBTS1-RI plants. The S. frugiperda resistance of transgenic tobacco plants was evaluated by in vitro toxicity test, and the results showed that the resistance of CBTS1-OE plants to S. frugiperda was significantly improved but that of CBTS1-RI plants was reduced. The P. nicotianae resistance of transgenic tobacco plants was assessed by the detached leaf assay, and the results showed that the resistance of CBTS1-OE plants to P. nicotianae was enhanced, while that of CBTS1-RI plants was attenuated. Further gene expression analysis showed that overexpression of CBTS1 increased the expression of the pathogen-related gene PR-1a, while silencing of CBTS1 decreased its expression. This study demonstrated that manipulating the expression of CBTS1 could change the cembranoid content in tobacco plants and alter their resistance to both insect pests and oomycete pathogens.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    URL: Article
    DOI: 10.3390/agronomy13030845
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 5
    Online Resource
    Online Resource
    The Critical Role of Additive Sulfate for Stable Alkaline Seawater Oxidation on Nickel‐Based Electrodes
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 42 ( 2021-10-11), p. 22922-22926
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 42 ( 2021-10-11), p. 22922-22926
    Abstract: Seawater electrolysis to produce hydrogen is a critical technology in marine energy projects; however, the severe anode corrosion caused by the highly concentrated chloride is a key issue should be addressed. In this work, we discover that the addition of sulfate in electrolyte can effectively retard the corrosion of chloride ions to the anode. We take nickel foam as the example and observe that the addition of sulfate can greatly improve the corrosion resistance, resulting in prolonged operating stability. Theoretical simulations and in situ experiments both demonstrate that sulfate anions can be preferentially adsorbed on anode surface to form a negative charge layer, which repulses the chloride ions away from the anode by electrostatic repulsion. The repulsive effect of the adsorbed sulfate is also applicable in highly‐active catalyst (nickel iron layered double hydroxide) on nickel foam, which shows ca. 5 times stability of that in traditional electrolyte.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.42
    DOI: 10.1002/ange.202110355
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 6
    Online Resource
    Online Resource
    Scalable Fabrication of Cu 2 S@NiS@Ni/NiMo Hybrid Cathode for High‐Performance Seawater Electrolysis
    Wiley ; 2023
    In:  Advanced Functional Materials Vol. 33, No. 37 ( 2023-09)
    Details
    In: Advanced Functional Materials, Wiley, Vol. 33, No. 37 ( 2023-09)
    Abstract: Electrochemical hydrogen evolution reaction (HER) with cost‐effectiveness, high performance, and repeatable scale‐up production hold promises for large‐scale green hydrogen generation technology. Herein, a convenient method for scaling up Cu 2 S@NiS@Ni/NiMo electrocatalysts on Cu foam with high geometric area over 100 cm 2 is presented. The hybrid electrode exhibits high hydrogen evolution activity with 190 and 250 mV overpotential at 1000 mA cm −2 and superior stability with negligible overpotential loss after over 2000 h at 500 mA cm −2 under steady‐state conditions in both artificial seawater and real seawater. Detailed characterizations and simulations demonstrate that high intrinsic activity resulting from the unique boundary interface, enhance mass transport resulting from superaerophobic nanoarray architecture, and corrosion resistance resulting from polyanion‐rich passivating layers together lead to the outstanding performance. The practicability is also demonstrated in an alkaline seawater electrolyzer coupling with the hybrid electrode and stable commercial anode.
    Type of Medium: Online Resource
    ISSN: 1616-301X , 1616-3028
    URL: Issue
    URL: Article
    DOI: 10.1002/adfm.v33.37
    DOI: 10.1002/adfm.202302263
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2029061-5
    detail.hit.zdb_id: 2039420-2
    SSG: 11
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  • 7
    Online Resource
    Online Resource
    Autobiography writing instruction: The journey of a teacher participating in a systemic functional linguistics genre pedagogy professional development
    Elsevier BV ; 2021
    In:  System Vol. 97 ( 2021-04), p. 102429-
    Details
    In: System, Elsevier BV, Vol. 97 ( 2021-04), p. 102429-
    Type of Medium: Online Resource
    ISSN: 0346-251X
    URL: Article
    DOI: 10.1016/j.system.2020.102429
    RVK:
    EQ 1000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 1500775-3
    detail.hit.zdb_id: 189289-7
    SSG: 7,11
    SSG: 5,3
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  • 8
    Online Resource
    Online Resource
    Translanguaging design in a third grade Chinese Language Arts class
    Walter de Gruyter GmbH ; 2022
    In:  Applied Linguistics Review Vol. 13, No. 3 ( 2022-05-25), p. 327-343
    Details
    In: Applied Linguistics Review, Walter de Gruyter GmbH, Vol. 13, No. 3 ( 2022-05-25), p. 327-343
    Abstract: Traditionally strict language separation policies in dual language bilingual education (DLBE) programs reflect parallel monolingualism and have been criticized as failing to recognize the sociolinguistic realities of bilingual students (García, Ofelia & Angel M. Y. Lin. 2017. Translanguaging in bilingual education. In Ofelia García, Angel M. Y. Lin & Stephen May (eds.), Bilingual and multilingual education , 117–130. Cham: Springer International Publishing). To recognize the dynamic nature of bilingualism and leverage bilingual learners’ full semiotic and linguistic repertoire as a resource, this study looks at how to strategically and purposefully develop flexible and multilingual educational spaces in a third grade Chinese Language Arts (CLA) class in a Mandarin-English DLBE program in the New England area, U.S. Drawing upon Sánchez, María Teresa (Maite), Ofelia García & Cristian Solorza. 2018. Reframing language allocation policy in dual language bilingual education. Bilingual Research Journal 41(1). 37–51. https://doi.org/10.1080/15235882.2017.1405098 , translanguaging allocation policy framework, the researcher and the teacher co-designed and implemented translanguaging documentation, translanguaging rings, and translanguaging transformation spaces in the CLA class throughout the school year of 2018–19. Taking the form of participatory design research (Bang, Megan & Shirin Vossoughi. 2016. Participatory design research and educational justice: Studying learning and relations within social change making. Cognition and Instruction 34(3). 173–193. https://doi.org/10.1080/07370008.2016.1181879 ), this collaborative inquiry demonstrates that translanguaging pedagogies could promote student engagement, contribute to their academic learning, and build home-school connections. It aims to provide authentic, sustainable knowledge for both researchers and practitioners to better serve bilingual learners in DLBE programs.
    Type of Medium: Online Resource
    ISSN: 1868-6303 , 1868-6311
    URL: Article
    DOI: 10.1515/applirev-2021-0024
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2575065-3
    SSG: 7,11
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  • 9
    Online Resource
    Online Resource
    Translanguaging: a pedagogy of heteroglossic hope
    Informa UK Limited ; 2023
    In:  International Journal of Bilingual Education and Bilingualism Vol. 26, No. 8 ( 2023-09-14), p. 919-923
    Details
    In: International Journal of Bilingual Education and Bilingualism, Informa UK Limited, Vol. 26, No. 8 ( 2023-09-14), p. 919-923
    Type of Medium: Online Resource
    ISSN: 1367-0050 , 1747-7522
    URL: Article
    DOI: 10.1080/13670050.2023.2212835
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2004789-7
    SSG: 7,11
    SSG: 5,3
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  • 10
    Online Resource
    Online Resource
    Chinese-English dual language bilingual education in the United States美国中英双语沉浸式教学
    Informa UK Limited ; 2022
    In:  Journal of Multilingual and Multicultural Development Vol. 43, No. 6 ( 2022-07-03), p. 477-486
    Details
    In: Journal of Multilingual and Multicultural Development, Informa UK Limited, Vol. 43, No. 6 ( 2022-07-03), p. 477-486
    Type of Medium: Online Resource
    ISSN: 0143-4632 , 1747-7557
    URL: Article
    DOI: 10.1080/01434632.2022.2092627
    RVK:
    EQ 1000
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2022
    detail.hit.zdb_id: 136713-4
    detail.hit.zdb_id: 1480742-7
    SSG: 7,11
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