In:
ChemPhysChem, Wiley, Vol. 7, No. 5 ( 2006-05-12), p. 1062-1067
Abstract:
Semiconductor quantum dots (QDs) as a kind of nonisotopic biological labeling material have many unique fluorescent properties relative to conventional organic dyes and fluorescent proteins, such as composition‐ and size‐dependent absorption and emission, a broad absorption spectrum, photostability, and single‐dot sensitivity. These properties make them a promising stable and sensitive label, which can be used for long‐term fluorescent tracking and subcellular location of genes and proteins. Here, a simple approach for the construction of QD‐labeled DNA probes was developed by attaching thiol‐ssDNA to QDs via a metal–thiol bond. The as‐prepared QD‐labeled DNA probes had high dispersivity, bioactivity, and specificity for hybridization. Based on such a kind of probe with a sequence complementary to multiple clone sites in plasmid pUC18, fluorescence in situ hybridization of the tiny bacterium Escherichia coli has been realized for the first time.
Type of Medium:
Online Resource
ISSN:
1439-4235
,
1439-7641
DOI:
10.1002/cphc.200500608
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2025223-7
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