GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Reactivation of Neutralized HIV-1 by Dendritic Cells Is Dependent on the Epitope Bound by the Antibody
    The American Association of Immunologists ; 2015
    In:  The Journal of Immunology Vol. 195, No. 8 ( 2015-10-15), p. 3759-3768
    Details
    In: The Journal of Immunology, The American Association of Immunologists, Vol. 195, No. 8 ( 2015-10-15), p. 3759-3768
    Abstract: Ab-neutralized HIV-1 can be captured by dendritic cells (DCs), which subsequently transfer infectious HIV-1 to susceptible CD4+ T cells. In this study, we examined the capacity of early Abs, as well as recently identified broadly neutralizing Abs (bNAbs) targeting different envelope glycoprotein (Env) epitopes, to block HIV-1 transmission by immature and mature DCs to HIV-1–sensitive cells. Three bNAbs directed against the gp41 membrane proximal region of Env (2F5, 4E10, and 10E8) and three gp120 bNAbs targeting the CD4 binding site (b12, VRC01, and NIH45-46) were examined. In addition, eight glycan-dependent bNAbs targeting the V1V2 apex (PG9, PG16, and PGT145), the V3 loop (2G12, PGT121, and PGT128), and the gp120–gp41 interface of Env (PGT151 and 35O22) were tested. bNAbs that bound specific glycans showed, depending on the immature or mature state of the DC, diverse efficiencies in HIV-1 trans-infection. All bNAbs that bound the CD4 binding site blocked trans-infection, whereas all bNAbs directed against the membrane proximal region lost neutralizing activity after DC-mediated HIV-1 transmission. To understand how preneutralized HIV-1 can be transferred as infectious virus by DCs, we followed the processing of 2F5-treated HIV-1 by DCs with confocal microscopy. Inhibition of DC-internalization pathways could not reverse the dissociation of 2F5 from HIV-1, suggesting that Ab dissociation occurs directly at the plasma membrane. Collectively, these findings imply that the location of the epitope and the neutralization capacity of these Abs determine the efficiency of DC-mediated HIV-1 transfer.
    Type of Medium: Online Resource
    ISSN: 0022-1767 , 1550-6606
    URL: Article
    DOI: 10.4049/jimmunol.1402344
    RVK:
    XA 54100
    RVK:
    XA 10000
    Language: English
    Publisher: The American Association of Immunologists
    Publication Date: 2015
    detail.hit.zdb_id: 1475085-5
    detail.hit.zdb_id: 3056-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Vanishing white matter: deregulated integrated stress response as therapy target
    Wiley ; 2019
    In:  Annals of Clinical and Translational Neurology Vol. 6, No. 8 ( 2019-08), p. 1407-1422
    Details
    In: Annals of Clinical and Translational Neurology, Wiley, Vol. 6, No. 8 ( 2019-08), p. 1407-1422
    Abstract: Vanishing white matter (VWM) is a fatal, stress‐sensitive leukodystrophy that mainly affects children and is currently without treatment. VWM is caused by recessive mutations in eukaryotic initiation factor 2B (eIF2B) that is crucial for initiation of mRNA translation and its regulation during the integrated stress response (ISR). Mutations reduce eIF2B activity. VWM pathomechanisms remain unclear. In contrast with the housekeeping function of eIF2B, astrocytes are selectively affected in VWM. One study objective was to test our hypothesis that in the brain translation of specific mRNAs is altered by eIF2B mutations, impacting primarily astrocytes. The second objective was to investigate whether modulation of eIF2B activity could ameliorate this altered translation and improve the disease. Methods Mice with biallelic missense mutations in eIF2B that recapitulate human VWM were used to screen for mRNAs with altered translation in brain using polysomal profiling. Findings were verified in brain tissue from VWM patients using qPCR and immunohistochemistry. The compound ISRIB (for “ISR inhibitor”) was administered to VWM mice to increase eIF2B activity. Its effect on translation, neuropathology, and clinical signs was assessed. Results In brains of VWM compared to wild‐type mice we observed the most prominent changes in translation concerning ISR mRNAs; their expression levels correlated with disease severity. We substantiated these findings in VWM patients’ brains. ISRIB normalized expression of mRNA markers, ameliorated brain white matter pathology and improved motor skills in VWM mice. Interpretation The present findings show that ISR deregulation is central in VWM pathomechanisms and a viable target for therapy.
    Type of Medium: Online Resource
    ISSN: 2328-9503 , 2328-9503
    URL: Issue
    URL: Article
    DOI: 10.1002/acn3.v6.8
    DOI: 10.1002/acn3.50826
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2740696-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Interaction of initiation factors with the cap structure of chimaeric mRNA containing the 5′‐untranslated regions of Semliki Forest virus RNA is related to translational efficiency
    Wiley ; 1992
    In:  European Journal of Biochemistry Vol. 208, No. 3 ( 1992-09), p. 581-587
    Details
    In: European Journal of Biochemistry, Wiley, Vol. 208, No. 3 ( 1992-09), p. 581-587
    Abstract: Chimaeric chloramphenicol acetyltransferase (CAT) mRNA, containing the leader sequences of genomic 42S RNA and subgenomic 26S RNA of Semliki Forest virus (SFV) were synthesized by in‐vitro transcription. These transcripts were translated with different efficiencies, as the authentic mRNA in SFV‐infected cells. Therefore, they can be used as model mRNA species to study the mechanism underlying SFV‐directed shut off of host protein synthesis. The interaction of translation initiation factors with the 5′ cap structure was studied. Transcripts prepared in vitro using T7 RNA polymrase were capped and methylated posttranscriptionally with [ 32 P]‐GTP and S ‐adenosyl‐ l ‐methionine to yield cap‐labelled mRNA species. Irradiation with ultraviolet light of 26S CAT and 42S CAT transcripts, together with crude rabbit reticulocyte initiation factors, resulted in the capspecific cross‐linking of eukaryotic initiation factors (eIF) eIF‐4E and eIF‐4B. The relative binding efficiency of these two factors to the cap structure of the various transcripts was, however, markedly different; the cap structure present in 26S CAT mRNA interacted efficiently with cap‐binding proteins, whereas the cap structure of 42S CAT mRNA hardly bound to these proteins. Comparable results were obtained under competitive conditions. Data are presented that the secondary structure close to the 5′ cap structure determines the efficiency of recognition of the mRNA by these initiation factors. Using a chemical cross‐linking assay, it was demonstrated that eIF‐4F, and also eIF‐4E, differentially interacted with the cap structure of the various transcripts. The data are discussed with respect to the possible mechanisms involved in SFV‐induced shut off of host cell protein synthesis.
    Type of Medium: Online Resource
    ISSN: 0014-2956 , 1432-1033
    URL: Issue
    URL: Article
    DOI: 10.1111/ejb.1992.208.issue-3
    DOI: 10.1111/j.1432-1033.1992.tb17222.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 1992
    detail.hit.zdb_id: 1398347-7
    detail.hit.zdb_id: 1464377-7
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Initiation of protein synthesis in eukaryotes
    Springer Science and Business Media LLC ; 1994
    In:  Molecular Biology Reports Vol. 19, No. 3 ( 1994-5), p. 139-145
    Details
    In: Molecular Biology Reports, Springer Science and Business Media LLC, Vol. 19, No. 3 ( 1994-5), p. 139-145
    Type of Medium: Online Resource
    ISSN: 0301-4851 , 1573-4978
    URL: Article
    DOI: 10.1007/BF00986956
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 1994
    detail.hit.zdb_id: 186544-4
    detail.hit.zdb_id: 1478217-0
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Role of the 3′ untranslated region of baculovirus p10 mRNA in high-level expression of foreign genes
    Microbiology Society ; 1999
    In:  Journal of General Virology Vol. 80, No. 8 ( 1999-08-01), p. 2253-2262
    Details
    In: Journal of General Virology, Microbiology Society, Vol. 80, No. 8 ( 1999-08-01), p. 2253-2262
    Abstract: The p10 gene of Autographa californica nucleopolyhedrovirus has two putative AATAAA polyadenylation signals. The downstream signal is used predominantly, as was determined by analysing 3′ cDNA ends. This downstream motif is followed by a GT-rich sequence, known to be important for efficient polyadenylation in mammalian systems. To analyse the importance of polyadenylation for p10 gene expression, recombinant viruses with altered 3′ untranslated regions (UTRs) were tested using chloramphenicol acetyltransferase (CAT) as a reporter. Surprisingly, after inactivation of the downstream AATAAA motif, CAT expression remained at the same high level as observed with a wild-type 3′ UTR. Polyadenylation occurred 24–28 nucleotides further downstream, probably due to an ATTAAA sequence motif. Replacing the p10 3′ UTR with the SV40 early terminator sequence as part of an hsp70– lacZ –SV40 gene cassette, which is commonly used in baculovirus expression vectors, resulted in a reduction in reporter gene expression. Polyadenylation occurred far more efficiently in the original p10 3′ UTR than in the SV40 terminator sequence, as was shown by testing the SV40 terminator separately. These results indicate that in order to obtain high levels of foreign gene expression, vectors that provide a wild-type p10 3′ UTR are to be preferred over those containing the hsp70– lacZ –SV40 gene cassette. Comparison of the p10 genes of various baculoviruses showed the presence of at least one AATAAA or ATTAAA motif in combination with a GT-rich sequence in the 3′ UTR, suggesting an evolutionary conservation of these two elements, thereby maintaining the high level of p10 gene expression.
    Type of Medium: Online Resource
    ISSN: 0022-1317 , 1465-2099
    URL: Article
    DOI: 10.1099/0022-1317-80-8-2253
    RVK:
    XA 53770
    RVK:
    WA 15000
    Language: English
    Publisher: Microbiology Society
    Publication Date: 1999
    detail.hit.zdb_id: 219316-4
    detail.hit.zdb_id: 2007065-2
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Cloning and analysis of cDNAs encoding the hypusine‐containing protein eIF5A of two lepidopteran insect species
    Wiley ; 1999
    In:  Insect Molecular Biology Vol. 8, No. 4 ( 1999-11), p. 531-538
    Details
    In: Insect Molecular Biology, Wiley, Vol. 8, No. 4 ( 1999-11), p. 531-538
    Abstract: Eukaryotic initiation factor eIF5A is essential for cell viability and contains a characteristic post‐translational modification of a specific lysine residue into a hypusine. cDNAs with similarity to eIF5A sequences were derived from Spodoptera exigua and S. frugiperda cDNA libraries. The deduced amino acid sequences are identical for both species and predict a protein with a molecular mass of 17.5 kDa. The Drosophila melanogaster eIF5A cDNA sequence was retrieved from the Drosophila EST Project. The predicted protein is 80% similar to Spodoptera eIF5A. A single eIF5A gene copy is present in the S. frugiperda genome, which is transcribed into four different transcripts. Infection of S. frugiperda cells with a baculovirus resulted in a strong decline of all four transcripts already at 12 h after infection. In contrast, the eIF5A protein was fairly stable up to 48 h post infection.
    Type of Medium: Online Resource
    ISSN: 0962-1075 , 1365-2583
    URL: Article
    DOI: 10.1046/j.1365-2583.1999.00148.x
    Language: English
    Publisher: Wiley
    Publication Date: 1999
    detail.hit.zdb_id: 33602-6
    detail.hit.zdb_id: 2020348-2
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Glia-Specific Activation of All Pathways of the Unfolded Protein Response in Vanishing White Matter Disease
    Oxford University Press (OUP) ; 2006
    In:  Journal of Neuropathology and Experimental Neurology Vol. 65, No. 7 ( 2006-07), p. 707-715
    Details
    In: Journal of Neuropathology and Experimental Neurology, Oxford University Press (OUP), Vol. 65, No. 7 ( 2006-07), p. 707-715
    Type of Medium: Online Resource
    ISSN: 0022-3069
    URL: Article
    DOI: 10.1097/01.jnen.0000228201.27539.50
    RVK:
    XA 55250
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2006
    detail.hit.zdb_id: 3088-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Solubilization by Cholate or Deoxycholate of a Dicyclohexylcarbodi-imide-Sensitive Adenosine Triphosphatase Complex from Escherichia coli
    Portland Press Ltd. ; 1974
    In:  Biochemical Society Transactions Vol. 2, No. 3 ( 1974-06-01), p. 512-513
    Details
    In: Biochemical Society Transactions, Portland Press Ltd., Vol. 2, No. 3 ( 1974-06-01), p. 512-513
    Type of Medium: Online Resource
    ISSN: 0300-5127 , 1470-8752
    URL: Article
    DOI: 10.1042/bst0020512
    Language: English
    Publisher: Portland Press Ltd.
    Publication Date: 1974
    detail.hit.zdb_id: 184237-7
    detail.hit.zdb_id: 2007367-7
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Infectious Bursal Disease Virus Capsid Protein VP3 Interacts both with VP1, the RNA-Dependent RNA Polymerase, and with Viral Double-Stranded RNA
    American Society for Microbiology ; 2002
    In:  Journal of Virology Vol. 76, No. 22 ( 2002-11-15), p. 11301-11311
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 76, No. 22 ( 2002-11-15), p. 11301-11311
    Abstract: Infectious bursal disease virus (IBDV) is a double-stranded RNA (dsRNA) virus of the Birnaviridae family. Its two genome segments are encapsidated together with multiple copies of the viral RNA-dependent RNA polymerase, VP1, in a single-shell capsid that is composed of VP2 and VP3. In this study we identified the domains responsible for the interaction between VP3 and VP1. Using the yeast two-hybrid system we found that VP1 binds to VP3 through an internal domain, while VP3 interacts with VP1 solely by its carboxy-terminal 10 amino acids. These results were confirmed by using a reverse-genetics system that allowed us to analyze the interaction of carboxy-terminally truncated VP3 molecules with VP1 in infected cells. Coimmunoprecipitations with VP1- and VP3-specific antibodies revealed that the interaction is extremely sensitive to truncation of VP3. The mere deletion of the C-terminal residue reduced coprecipitation almost completely and also fully abolished production of infectious virions. Surprisingly, these experiments additionally revealed that VP3 also binds to RNA. RNase treatments and reverse transcription-PCR analyses of the immunoprecipitates demonstrated that VP3 interacts with dsRNA of both viral genome segments. This interaction is not mediated by the carboxy-terminal domain of VP3 since C-terminal truncations of 1, 5, or 10 residues did not prevent formation of the VP3-dsRNA complexes. VP3 seems to be the key organizer of birnavirus structure, as it maintains critical interactions with all components of the viral particle: itself, VP2, VP1, and the two genomic dsRNAs.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/JVI.76.22.11301-11311.2002
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2002
    detail.hit.zdb_id: 1495529-5
    detail.hit.zdb_id: 80174-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Dendritic Cells Preferentially Transfer CXCR4-Using Human Immunodeficiency Virus Type 1 Variants to CD4 + T Lymphocytes in trans
    American Society for Microbiology ; 2008
    In:  Journal of Virology Vol. 82, No. 16 ( 2008-08-15), p. 7886-7896
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 82, No. 16 ( 2008-08-15), p. 7886-7896
    Abstract: Human immunodeficiency virus type 1 (HIV-1) preferentially utilizes the CCR5 coreceptor for target cell entry in the acute phase of infection, while later in disease progression the virus switches to the CXCR4 coreceptor in approximately 50% of patients. In response to HIV-1 the adaptive immune response is triggered, and antibody (Ab) production is elicited to block HIV-1 entry. We recently determined that dendritic cells (DCs) can efficiently capture Ab-neutralized HIV-1, restore infectivity, and transmit infectious virus to target cells. Here, we tested the effect of Abs on trans transmission of CCR5 or CXCR4 HIV-1 variants. We observed that transmission of HIV-1 by immature as well as mature DCs was significantly higher for CXCR4- than CCR5-tropic viral strains. Additionally, neutralizing Abs directed against either the gp41 or gp120 region of the envelope such as 2F5, 4E10, and V3-directed Abs inhibited transmission of CCR5-tropic HIV-1, whereas Ab-treated CXCR4-tropic virus demonstrated unaltered or increased transmission. To further study the effects of coreceptor usage we tested molecularly cloned HIV-1 variants with modifications in the envelope that were based on longitudinal gp120 V1 and V3 variable loop sequences from a patient progressing to AIDS. We observed that DCs preferentially facilitated infection of CD4 + T lymphocytes of viral strains with an envelope phenotype found late in disease. Taken together, our results illustrate that DCs transmit CXCR4-tropic HIV-1 much more efficiently than CCR5 strains; we hypothesize that this discrimination could contribute to the in vivo coreceptor switch after seroconversion and could be responsible for the increase in viral load.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/JVI.00245-08
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2008
    detail.hit.zdb_id: 1495529-5
    detail.hit.zdb_id: 80174-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...