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  • 1
    Online Resource
    Online Resource
    Phenotypic Characterization of Arabidopsis Ascorbate and Glutathione Deficient Mutants under Abiotic Stresses
    MDPI AG ; 2021
    In:  Agronomy Vol. 11, No. 4 ( 2021-04-14), p. 764-
    Details
    In: Agronomy, MDPI AG, Vol. 11, No. 4 ( 2021-04-14), p. 764-
    Abstract: Ascorbic acid (AsA) and glutathione (GSH) are considered important factors to protect plants against abiotic stress. To investigate whether altered endogenous GSH and AsA affect seed germination, plant performance and the abiotic stress tolerance, GSH deficient mutant cad2-1 and AsA-deficient mutants (vtc2-4 and vtc5-2) were phenotypically characterized for their seed germination, shoot growth, photosynthetic activity and root architecture under abiotic stresses. The cad2-1, vtc2-4 and vtc5-2 mutants showed a decrease in osmotic and salt stress tolerance, in sensitivity to ABA during seed germination, and in plant performance under severe abiotic stresses. GSH deficiency in the cad2-1 plants affected plant growth and root development in plants exposed to strong drought, oxidative and heavy metal stress conditions. Plants with lower GSH did not show an increased sensitivity to strong salt stress (100 mM NaCl). In contrast, the mutants with lower AsA enhanced salt stress tolerance in the long-term exposures to strong salt stress since they showed larger leaf areas, longer primary roots and more lateral root numbers. Limitations on AsA or GSH synthesis had no effect on photosynthesis in plants exposed to long-term strong salt or drought stresses, whereas they effected on photosynthesis of mutants exposed to CdCl2. Taken together, the current study suggests that AsA and GSH are important for seed germination, root architecture, shoot growth and plant performance in response to different abiotic stresses, and their functions are dependent on the stress-inducing agents and the stress levels.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    URL: Article
    DOI: 10.3390/agronomy11040764
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 2
    Online Resource
    Online Resource
    Produce Japanese Encephalitis standard antiserum of strain Nakayama for vaccine quality control
    National Institute for Control of Vaccines and Biologicals ; 2021
    In:  JOURNAL OF CONTROL VACCINES AND BIOLOGICALS , No. 1 ( 2021-12-31), p. 78-88
    Details
    In: JOURNAL OF CONTROL VACCINES AND BIOLOGICALS, National Institute for Control of Vaccines and Biologicals, , No. 1 ( 2021-12-31), p. 78-88
    Abstract: Japanese Encephalitis vaccine has contributed to repel Japanese Encephalitis in young children in the national vaccination campaign. To qualitatively evaluate the protective efficiency of Japanese Encephalitis vaccine, a standard antiserum used as a positive control was performed in parallel with testing samples. A study was organized at National Institute for Control of Vaccines and Biologicals (NICVB) as following. Anti-sera were produced by 2-dose-injection of national standard vaccine to 28-day-old white mice, weighing 11- 13g. After blood collection and serum separation, the PRNT50 neutralizing antibody potency was evaluated in parallel with international standard antisera from NIBSC and antisera from Vabiotech manufacturer. Antiserum lot 0118 was produced with a neutralizing antibody potency PRNT50 of 1.74 to ensure criteria of a positive control standard for performing Japanese Encephalitis vaccine potency test.
    Type of Medium: Online Resource
    ISSN: 2815-6269
    URL: Issue
    URL: Article
    DOI: 10.56086/jcvb.vi1
    DOI: 10.56086/jcvb.vi1.7
    Language: Unknown
    Publisher: National Institute for Control of Vaccines and Biologicals
    Publication Date: 2021
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  • 3
    Online Resource
    Online Resource
    Geology, mineralogy, geochemistry and δ 34 S of sedimentary rock-hosted Au deposits in Song Hien structure, NE Vietnam
    Elsevier BV ; 2017
    In:  Ore Geology Reviews Vol. 84 ( 2017-04), p. 273-288
    Details
    In: Ore Geology Reviews, Elsevier BV, Vol. 84 ( 2017-04), p. 273-288
    Type of Medium: Online Resource
    ISSN: 0169-1368
    URL: Article
    DOI: 10.1016/j.oregeorev.2016.12.032
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2017
    detail.hit.zdb_id: 2029106-1
    SSG: 13
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  • 4
    Online Resource
    Online Resource
    Ca lâm sàng: U hạch thần kinh tuyến thượng thận phát hiện tình cờ
    108 Institute of Clinical Medical and Pharmaceutical Sciences ; 2021
    In:  Journal of 108 - Clinical Medicine and Phamarcy Vol. 16, No. 5 ( 2021-07-31)
    Details
    In: Journal of 108 - Clinical Medicine and Phamarcy, 108 Institute of Clinical Medical and Pharmaceutical Sciences, Vol. 16, No. 5 ( 2021-07-31)
    Abstract: U hạch thần kinh là khối u lành tính xuất phát từ chuỗi hạch giao cảm lớn, thường gặp ở trung thất sau và sau phúc mạc. U hạch thần kinh tại tuyến thượng thận là một khối u rất hiếm gặp, không có triệu chứng và hầu hết được phát hiện tình cờ. Khối u tuy lành tính nhưng có thể phát triển gây chèn ép nên phẫu thuật là phương pháp điều trị tối ưu. Việc chẩn đoán xác định trước phẫu thuật rất khó khăn, dễ nhầm lẫn với u thượng thận ác tính. Tiên lượng u hạch thần kinh sau phẫu thuật là tốt và chỉ cần theo dõi định kỳ mà không cần điều trị gì thêm. Chúng tôi báo cáo trường hợp lâm sàng u hạch thần kinh tuyến thượng thận phát hiện tình cờ, chưa có chẩn đoán xác định trước mổ, chưa loại trừ ung thư tuyến thượng thận. Sau phẫu thuật lấy u, bệnh nhân phục hồi tốt, chẩn đoán xác định khối u bằng giải phẫu bệnh. Kết luận: U hạch thần kinh tuyến thượng thận là khối u lành tính, rất hiếm gặp, chẩn đoán trước mổ khó khăn, phẫu thuật là phương pháp điều trị duy nhất, giải phẫu bệnh là phương pháp chính trong chẩn đoán.
    Type of Medium: Online Resource
    ISSN: 1859-2872
    URL: Issue
    URL: Article
    DOI: 10.52389/ydls.v16i5
    DOI: 10.52389/ydls.v16i5.812
    Language: Unknown
    Publisher: 108 Institute of Clinical Medical and Pharmaceutical Sciences
    Publication Date: 2021
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  • 5
    Online Resource
    Online Resource
    Biological activities and hairy roots induction of Abutilon indicum (L.)
    Viet Nam National University Ho Chi Minh City ; 2016
    In:  Science and Technology Development Journal Vol. 19, No. 4 ( 2016-12-31), p. 95-104
    Details
    In: Science and Technology Development Journal, Viet Nam National University Ho Chi Minh City, Vol. 19, No. 4 ( 2016-12-31), p. 95-104
    Abstract: Abutilon indicum L. belonging to Malvaceae is used as traditional medicinal herbs to treat syphilis, hypoglycemia, hepatic disorders, and malaria... Because of the medicinal value of A. indicum, the aims of this study are the evaluation of bioactivities of A. indicum and production of transformed hairy root for pharmaceutical production. In this study, the reducing power of ethanol root extract and stem extract are higher than that of leave. The root extract exhibited potent brine shrimp (Artemia salina) lethality with LC50 37.04 μg/mL. The α-glucosidase and acetylcholinesterase inhibitory activities of the root extract are the highest. These results showed that roots are higher bioactivities than stems and leaves. This study induced successfully hairy roots of A. indicum L. via Agrobacterium rhizogenes ATCC 15834 in the plant cells. The frequency of hairy root and number of hairy root induction from the wounded sites of leaves are the highest (88.66 % and 8.66 roots). The stable introduction of the rolB and rolC genes of A. rhizogenes strain 15834 into A. indicum plants was confirmed by PCR analysis. Besides, the absence of the virD gene confirmed hairy roots as a bacteria-free. Subsequently, these results demonstrated that A. indicum, particularly the roots, have great potential as pharmacological values and hairy root production maybe used for pharmaceutical sources.
    Type of Medium: Online Resource
    ISSN: 1859-0128 , 1859-0128
    URL: Article
    DOI: 10.32508/stdj.v19i4.602
    Language: Unknown
    Publisher: Viet Nam National University Ho Chi Minh City
    Publication Date: 2016
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  • 6
    Online Resource
    Online Resource
    Chemistry and Molecular Characteristics of Premna flavescens Wall. ex C. B. Clarke in Vietnam
    SAGE Publications ; 2021
    In:  Natural Product Communications Vol. 16, No. 9 ( 2021-09), p. 1934578X2110314-
    Details
    In: Natural Product Communications, SAGE Publications, Vol. 16, No. 9 ( 2021-09), p. 1934578X2110314-
    Abstract: Premna flavescens Wall. ex C. B. Clarke, a medicinal plant in the family Lamiaceae, was used for the treatment of arthritis in folk medicine. From the essential oil extracted from the leaves of this plant we have identified 51 components, with β-caryophyllene as the major component (26.3%). The essential oil showed a strong anti-inflammatory effect in vitro with an IC 50 of 5.88 µg/mL. DNA barcoding is an useful tool for species identification based on the standardized genomic DNA fragments, but the molecular database of P flavescens is still lacking from Genebank. A molecular species identification tool for P flavescens was developed for the first time using DNA barcoding. The sequences of rbcL, trnH–psbA, internal transcribed spacer, and 18S barcodes of P flavescens were submitted to the Genebank with the accession numbers MW553265, MW553266, MT935698.1, and MW485128.1, respectively. This will be highly useful for the molecular authentication of the marker samples.
    Type of Medium: Online Resource
    ISSN: 1934-578X , 1555-9475
    URL: Article
    DOI: 10.1177/1934578X211031410
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2430442-6
    SSG: 15,3
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  • 7
    Online Resource
    Online Resource
    Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor
    MDPI AG ; 2020
    In:  Plants Vol. 9, No. 4 ( 2020-04-23), p. 548-
    Details
    In: Plants, MDPI AG, Vol. 9, No. 4 ( 2020-04-23), p. 548-
    Abstract: Aeroponics is considered as a potential method for the culture of herbal plants due to the high growth rate, quantity and quality enhancement of secondary metabolites, and substantial environmental progress associated with this method. The aim of this study was to develop a sufficient protocol for successful Urena lobata hairy root induction by Agrobacterium rhizogenes ATCC 15834, using a precursor and elicitor to enhance α-glucosidase inhibitory activity (GIA) of aeroponic hairy roots (AHRs) in greenhouse conditions. In this study, we found that the optimized procedure (10 min, Woody plant medium (WPM), 1/25 salt strength) had an outstanding effect with a reduction in the rooting time (RT), promotion of the rooting rate (RR), and increase in the fresh weight (FW) and dry weight (DW) compared with the original procedure (30 min, Murashige and Skoog (MS) medium, 1/25 salt strength) after 30 days of culture. The highest DW, GIA, flavonoid (FLA) and phenolic (PHEL) contents were observed for individual addition of 10 mM phenylalanine (PA) or 50 mM chitosan (CS) in the late exponential phase (eighth week) with 15 days of elicitation compared to the control AHRs. However, individual treatment was less effective than the combination of the two. Positive correlations among the GIA, FLA and PHEL indicate that AHRs accumulated phenolic compounds, leading to an increase in the GIA by a synergistic effect. In conclusion, the culture of Urena lobata AHRs with PA and CS is an efficient procedure to produce GIA material in greenhouse conditions.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    URL: Article
    DOI: 10.3390/plants9040548
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2704341-1
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  • 8
    Online Resource
    Online Resource
    VOLATILE COMPOUNDS AND BIOLOGICAL ACTIVITIES OF ESSENTIAL OIL OF GLOBBA PENDULA ROXB. COLLECTED AT AN GIANG PROVINCE
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Science and Technology Vol. 58, No. 4 ( 2020-07-13), p. 434-
    Details
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 58, No. 4 ( 2020-07-13), p. 434-
    Abstract: Twenty-five compounds were identified from the essential oil of Globba pendula Roxb. by gas chromatography–mass spectrometry (GC-MS) with Selinene 〈 d- 〉 (22.09%) as the main component, followed by Ishwarane (6.52%). The essential oil was found to possess moderate NO inhibitory effect with IC50 of 41.68 ±4.51 µg/ml, significant cytotoxic activity against MCF7 and Hep3B cell lines with IC50 of 28.15±1.08 and 35.24±0.06 (µg/ml), respectively. This is the first report on volatile compounds and biological activities of essential oil of Globba pendula Roxb. collected at An Giang province, Vietnam.
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    URL: Article
    DOI: 10.15625/2525-2518/58/4/14856
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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  • 9
    Online Resource
    Online Resource
    In Vitro and In Vivo Anti-Inflammatory Activity of the Rhizomes of Globba pendula Roxb
    SAGE Publications ; 2021
    In:  Natural Product Communications Vol. 16, No. 10 ( 2021-10), p. 1934578X2110559-
    Details
    In: Natural Product Communications, SAGE Publications, Vol. 16, No. 10 ( 2021-10), p. 1934578X2110559-
    Abstract: In this study, we aimed at evaluating in vitro and in vivo anti-inflammatory activity of various extracts of the rhizomes of Globba pendula Roxb. Three extracts ( n-hexane, ethyl acetate, and water) were screened for their inhibitory effect on NO production by lipopolysaccharide-stimulated RAW 264.7 macrophages. The ethyl acetate extract of G. pendula rhizomes (EGP) showed a potential effect with an IC 50 value of 32.45 µg/mL. For in vivo study, the ethyl acetate extract was further investigated for its anti-inflammatory effect using collagen antibody-induced arthritic mice (CAIA). The level of arthritis in experimental mice significantly reduced ( P  〈  .05) after treatment with EGP at a dose of 500 mg/kg body weight (b.w.). This study also revealed that EGP is orally non-toxic. Ethyl p-methoxy cinamate was identified as the main constituent of EGP, which may result in its anti-inflammatory effect.
    Type of Medium: Online Resource
    ISSN: 1934-578X , 1555-9475
    URL: Article
    DOI: 10.1177/1934578X211055907
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2430442-6
    SSG: 15,3
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  • 10
    Online Resource
    Online Resource
    Multiple endocrine neoplasia 2A with RET mutation p.Cys380Arg (GRCh38): A case report
    108 Institute of Clinical Medical and Pharmaceutical Sciences ; 2023
    In:  Journal of 108 - Clinical Medicine and Phamarcy ( 2023-02-27)
    Details
    In: Journal of 108 - Clinical Medicine and Phamarcy, 108 Institute of Clinical Medical and Pharmaceutical Sciences, ( 2023-02-27)
    Abstract: MEN 2A is a complex autosomal dominant inherited syndrome characterized by pheochromocytoma (PC), medullary thyroid carcinoma (MTC) and primary parathyroid hyperplasia (PHP). We report a case of 26 years old female offended with bilateral pheochromocytoma, medullary thyroid carcinoma and primary hyperparathyroidism due to multiple endocrine neoplasia type 2A confirmed by RET gene mutation p.Cys380Arg according to the GRCh38 (Genome Reference Consortium Humain Build 38 Organism - 2013) classification. The mutation with a change of amino acid 380 from Cys (Cysteine) to Arg (Arginin) is found in more than one-half of kindreds with MEN2A.
    Type of Medium: Online Resource
    ISSN: 1859-2872
    URL: Issue
    URL: Article
    DOI: 10.52389/ydls.v17iTA
    DOI: 10.52389/ydls.v17iTA.1652
    Language: Unknown
    Publisher: 108 Institute of Clinical Medical and Pharmaceutical Sciences
    Publication Date: 2023
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