In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 17, No. 12 ( 2021-12-23), p. e1009970-
Abstract:
Insecticide resistance is a major threat to gains in malaria control, which have been stalling and potentially reversing since 2015. Studies into the causal mechanisms of insecticide resistance are painting an increasingly complicated picture, underlining the need to design and implement targeted studies on this phenotype. In this study, we compare three populations of the major malaria vector An . coluzzii : a susceptible and two resistant colonies with the same genetic background. The original colonised resistant population rapidly lost resistance over a 6-month period, a subset of this population was reselected with pyrethroids, and a third population of this colony that did not lose resistance was also available. The original resistant, susceptible and re-selected colonies were subject to RNAseq and whole genome sequencing, which identified a number of changes across the transcriptome and genome linked with resistance. Firstly, an increase in the expression of genes within the oxidative phosphorylation pathway were seen in both resistant populations compared to the susceptible control; this translated phenotypically through an increased respiratory rate, indicating that elevated metabolism is linked directly with resistance. Genome sequencing highlighted several blocks clearly associated with resistance, including the 2Rb inversion. Finally, changes in the microbiome profile were seen, indicating that the microbial composition may play a role in the resistance phenotype. Taken together, this study reveals a highly complicated phenotype in which multiple transcriptomic, genomic and microbiome changes combine to result in insecticide resistance.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1009970
DOI:
10.1371/journal.pgen.1009970.g001
DOI:
10.1371/journal.pgen.1009970.g002
DOI:
10.1371/journal.pgen.1009970.g003
DOI:
10.1371/journal.pgen.1009970.g004
DOI:
10.1371/journal.pgen.1009970.g005
DOI:
10.1371/journal.pgen.1009970.g006
DOI:
10.1371/journal.pgen.1009970.g007
DOI:
10.1371/journal.pgen.1009970.s001
DOI:
10.1371/journal.pgen.1009970.s002
DOI:
10.1371/journal.pgen.1009970.s003
DOI:
10.1371/journal.pgen.1009970.s004
DOI:
10.1371/journal.pgen.1009970.s005
DOI:
10.1371/journal.pgen.1009970.s006
DOI:
10.1371/journal.pgen.1009970.s007
DOI:
10.1371/journal.pgen.1009970.s008
DOI:
10.1371/journal.pgen.1009970.s009
DOI:
10.1371/journal.pgen.1009970.s010
DOI:
10.1371/journal.pgen.1009970.s011
DOI:
10.1371/journal.pgen.1009970.s012
DOI:
10.1371/journal.pgen.1009970.s013
DOI:
10.1371/journal.pgen.1009970.s014
DOI:
10.1371/journal.pgen.1009970.s015
DOI:
10.1371/journal.pgen.1009970.r001
DOI:
10.1371/journal.pgen.1009970.r002
DOI:
10.1371/journal.pgen.1009970.r003
DOI:
10.1371/journal.pgen.1009970.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2186725-2
Permalink