In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 95, No. 4 ( 2013-12-06), p. 689-697
Abstract:
Inflammation results in the recruitment of neutrophils and monocytes, which is crucial for the healing process. In the present study, we used 19F MRI to monitor in vivo the infiltration of neutrophils and monocytes from the onset of inflammation to the resolution and healing phase. Matrigel, with or without LPS, was s.c.-implanted into C57BL/6 mice. This resulted in a focal inflammation lasting over a period of 20 days, with constantly decreasing LPS levels in doped matrigel plugs. After i.v. administration of 19F containing contrast agent, 19F MRI revealed a zonular 19F signal in the periphery of LPS containing matrigel plugs, which was not observed in control plugs. Analysis of the 19F signal over the observation period demonstrated the strongest 19F signal after 24 h, which decreased to nearly zero after 20 days. The 19F signal was mirrored by the amount of leukocytes in the matrigel, with neutrophils dominating at early time-points and macrophages at later time-points. Both populations were shown to take up the 19F contrast agent. In conclusion, 19F MRI, in combination with the matrigel/LPS model, permits the noninvasive analysis of neutrophil and monocyte infiltration over the complete course of inflammation in vivo.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2013
detail.hit.zdb_id:
2026833-6
SSG:
12
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