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  • 1
    Online Resource
    Online Resource
    Robust estimation of bacterial cell count from optical density
    Springer Science and Business Media LLC ; 2020
    In:  Communications Biology Vol. 3, No. 1 ( 2020-09-17)
    Details
    In: Communications Biology, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2020-09-17)
    Abstract: Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli . Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals   〈 1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
    Type of Medium: Online Resource
    ISSN: 2399-3642
    URL: Article
    DOI: 10.1038/s42003-020-01127-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2919698-X
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  • 2
    Online Resource
    Online Resource
    Author Correction: Robust estimation of bacterial cell count from optical density
    Springer Science and Business Media LLC ; 2020
    In:  Communications Biology Vol. 3, No. 1 ( 2020-10-27)
    Details
    In: Communications Biology, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2020-10-27)
    Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper.
    Type of Medium: Online Resource
    ISSN: 2399-3642
    URL: Article
    DOI: 10.1038/s42003-020-01371-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2919698-X
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  • 3
    Online Resource
    Online Resource
    The prevalence and characteristics of metabolic syndrome according to different definitions in China: a nationwide cross-sectional study, 2012–2015
    Springer Science and Business Media LLC ; 2022
    In:  BMC Public Health Vol. 22, No. 1 ( 2022-10-07)
    Details
    In: BMC Public Health, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2022-10-07)
    Abstract: Metabolic syndrome (MetS) is characterized by a cluster of signs of metabolic disturbance and has caused a huge burden on the health system. The study aims to explore the prevalence and characteristics of MetS defined by different criteria in the Chinese population. Methods Using the data of the China Hypertension Survey (CHS), a nationally representative cross-sectional study from October 2012 to December 2015, a total of 28,717 participants aged 35 years and above were included in the analysis. The MetS definitions of the International Diabetes Federation (IDF), the updated US National Cholesterol Education Program Adult Treatment Panel III (the revised ATP III), and the Joint Committee for Developing Chinese Guidelines (JCDCG) on Prevention and Treatment of Dyslipidemia in Adults were used. Multivariable logistic regression was used to identify factors associated with MetS. Results The prevalence of MetS diagnosed according to the definitions of IDF, the revised ATP III, and JCCDS was 26.4%, 32.3%, and 21.5%, respectively. The MetS prevalence in men was lower than in women by IDF definition (22.2% vs. 30.3%) and by the revised ATP III definition (29.2% vs. 35.4%), but the opposite was true by JCDCG (24.4%vs 18.5%) definition. The consistency between the three definitions for men and the revised ATP III definition and IDF definition for women was relatively good, with kappa values ranging from 0.77 to 0.89, but the consistency between the JCDCG definition and IDF definition (kappa = 0.58) and revised ATP III definition (kappa = 0.58) was poor. Multivariable logistic regression showed that although the impact and correlation intensity varied with gender and definition, area, age, education, smoking, alcohol use, and family history of cardiovascular disease were factors related to MetS. Conclusions The prevalence and characteristics of the MetS vary with the definition used in the Chinese population. The three MetS definitions are more consistent in men but relatively poor in women. On the other hand, even if estimated according to the definition of the lowest prevalence, MetS is common in China.
    Type of Medium: Online Resource
    ISSN: 1471-2458
    URL: Article
    DOI: 10.1186/s12889-022-14263-w
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2041338-5
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  • 4
    Online Resource
    Online Resource
    An analytical power frequency reactance calculation method of the triaxial high temperature superconducting cables
    Elsevier BV ; 2022
    In:  Energy Reports Vol. 8 ( 2022-11), p. 26-33
    Details
    In: Energy Reports, Elsevier BV, Vol. 8 ( 2022-11), p. 26-33
    Type of Medium: Online Resource
    ISSN: 2352-4847
    URL: Article
    DOI: 10.1016/j.egyr.2022.08.083
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2814795-9
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  • 5
    Online Resource
    Online Resource
    Three Musketeers for Lowering Cholesterol: Statins, Ezetimibe and Evolocumab
    Bentham Science Publishers Ltd. ; 2021
    In:  Current Medicinal Chemistry Vol. 28, No. 5 ( 2021-02), p. 1025-1041
    Details
    In: Current Medicinal Chemistry, Bentham Science Publishers Ltd., Vol. 28, No. 5 ( 2021-02), p. 1025-1041
    Abstract: Coronary heart disease (CHD) is closely related to hypercholesterolemia, and lowering serum cholesterol is currently the most important strategy in reducing CHD. In humans, the serum cholesterol level is determined mainly by three metabolic pathways, namely, dietary cholesterol intake, cholesterol synthesis, and cholesterol degradation in vivo. An intervention that targets the key molecules in the three pathways is an important strategy in lowering serum lipids. Statins inhibit 3-hydroxyl-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) to reduce low-density lipoprotein (LDL) by about 20% to 45%. However, up to 15% of patients cannot tolerate the potential side effects of high statin dosages, and several patients also still do not reach their optimal LDL goals after being treated with statins. Ezetimibe inhibits cholesterol absorption by targeting the Niemann–Pick C1-like 1 protein (NPC1L1), which is related to cholesterol absorption in the intestines. Ezetimibe lowers LDL by about 18% when used alone and by an additional 25% when combined with statin therapy. The proprotein convertase subtilisin/kexin type 9 (PCSK9) increases hepatic LDLR degradation, thereby reducing the liver’s ability to remove LDL, which can lead to hypercholesterolemia. Evolocumab, which is a PCSK9 monoclonal antibody, can reduce LDL from baseline by 53% to 56%. The three drugs exert lipid-lowering effects by regulating the three key pathways in lipid metabolism. Combining any with the two other drugs on the basis of statin treatment has improved the lipid-lowering effect. Whether the combination of the three musketeers will reduce the side effects of monotherapy and achieve the lipid-lowering effect should be studied further in the future.
    Type of Medium: Online Resource
    ISSN: 0929-8673
    URL: Article
    DOI: 10.2174/0929867327666200505091738
    Language: English
    Publisher: Bentham Science Publishers Ltd.
    Publication Date: 2021
    SSG: 15,3
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  • 6
    Online Resource
    Online Resource
    Adoption of Convolutional Neural Network Algorithm Combined with Augmented Reality in Building Data Visualization and Intelligent Detection
    Hindawi Limited ; 2021
    In:  Complexity Vol. 2021 ( 2021-6-29), p. 1-13
    Details
    In: Complexity, Hindawi Limited, Vol. 2021 ( 2021-6-29), p. 1-13
    Abstract: It aims to improve the degree of visualization of building data, ensure the ability of intelligent detection, and effectively solve the problems encountered in building data processing. Convolutional neural network and augmented reality technology are adopted, and a building visualization model based on convolutional neural network and augmented reality is proposed. The performance of the proposed algorithm is further confirmed by performance verification on public datasets. It is found that the building target detection model based on convolutional neural network and augmented reality has obvious advantages in algorithm complexity and recognition accuracy. It is 25 percent more accurate than the latest model. The model can make full use of mobile computing resources, avoid network delay and dependence, and guarantee the real-time requirement of data processing. Moreover, the model can also well realize the augmented reality navigation and interaction effect of buildings in outdoor scenes. To sum up, this study provides a research idea for the identification, data processing, and intelligent detection of urban buildings.
    Type of Medium: Online Resource
    ISSN: 1099-0526 , 1076-2787
    URL: Article
    DOI: 10.1155/2021/5161111
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2004607-8
    SSG: 11
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  • 7
    Online Resource
    Online Resource
    Parallel multicomponent interferometer with a spinor Bose-Einstein condensate
    American Physical Society (APS) ; 2019
    In:  Physical Review A Vol. 100, No. 1 ( 2019-7-15)
    Details
    In: Physical Review A, American Physical Society (APS), Vol. 100, No. 1 ( 2019-7-15)
    Type of Medium: Online Resource
    ISSN: 2469-9926 , 2469-9934
    URL: Article
    DOI: 10.1103/PhysRevA.100.013618
    RVK:
    UA 6500.3A
    Language: English
    Publisher: American Physical Society (APS)
    Publication Date: 2019
    detail.hit.zdb_id: 2844156-4
    detail.hit.zdb_id: 209769-2
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  • 8
    Online Resource
    Online Resource
    Non-oxidative modified low-density lipoproteins: The underappreciated risk factors for atherosclerosis
    Bentham Science Publishers Ltd. ; 2023
    In:  Current Medicinal Chemistry Vol. 31 ( 2023-08-07)
    Details
    In: Current Medicinal Chemistry, Bentham Science Publishers Ltd., Vol. 31 ( 2023-08-07)
    Abstract: Atherosclerosis, the pathological basis of most cardiovascular diseases, is a main risk factor causing about 20 million deaths each year worldwide. Oxidized low-density lipoprotein is recognized as the most important and independent risk factor in initiating and promoting atherosclerosis. Numerous antioxidants are extensively used in clinical practice, but they have no significant effect on reducing the morbidity and mortality of cardiovascular diseases. This finding suggests that researchers should pay more attention to the important role of non-oxidative modified low-density lipoprotein in atherosclerosis with a focus on oxidized low-density lipoprotein. This review briefly summarizes several important non-oxidative modified low-density lipoproteins associated with atherosclerosis, introduces the pathways through which these non-oxidative modified low-density lipoproteins induce the development of atherosclerosis in vivo, and discusses the mechanism of atherogenesis induced by these non-oxidative modified low-density lipoproteins. New therapeutic strategies and potential drug targets are provided for the prevention and treatment of atherosclerotic cardiovascular diseases.
    Type of Medium: Online Resource
    ISSN: 0929-8673
    URL: Article
    DOI: 10.2174/0929867331666230807154019
    Language: English
    Publisher: Bentham Science Publishers Ltd.
    Publication Date: 2023
    SSG: 15,3
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  • 9
    Online Resource
    Online Resource
    Chinese Public’s Engagement in Preventive and Intervening Health Behaviors During the Early Breakout of COVID-19: Cross-Sectional Study
    JMIR Publications Inc. ; 2020
    In:  Journal of Medical Internet Research Vol. 22, No. 8 ( 2020-8-21), p. e19995-
    Details
    In: Journal of Medical Internet Research, JMIR Publications Inc., Vol. 22, No. 8 ( 2020-8-21), p. e19995-
    Abstract: Since January 2020, the coronavirus disease (COVID-19) swept over China and then the world, causing a global public health crisis. People’s adoption of preventive and intervening behaviors is critical in curbing the spread of the virus. Objective The aim of this study is to evaluate Chinese people’s adoption of health behaviors in responding to COVID-19 and to identify key determinants for their engagement. Methods An anonymous online questionnaire was distributed in early February 2020 among Mainland Chinese (18 years or older) to examine their engagement in preventive behaviors (eg, frequent handwashing, wearing masks, staying at home) and intervening behaviors (eg, advising family to wash hands frequently), and to explore potential determinants for their adoption of these health behaviors. Results Out of 2949 participants, 55.3% (n=1629) reported frequent engagement in preventive health behaviors, and over 84% (n=2493) performed at least one intervening health behavior. Greater engagement in preventive behaviors was found among participants who received higher education, were married, reported fewer barriers and greater benefits of engagement, reported greater self-efficacy and emotional support, had greater patient-centered communication before, had a greater media literacy level, and had greater new media and traditional media use for COVID-19 news. Greater engagement in intervening behaviors was observed among participants who were married, had lower income, reported greater benefits of health behaviors, had greater patient-centered communication before, had a lower media literacy level, and had a greater new media and traditional media use for COVID-19 news. Conclusions Participants’ engagement in coronavirus-related preventive and intervening behaviors was overall high, and the associations varied across demographic and psychosocial variables. Hence, customized health interventions that address the determinants for health behaviors are needed to improve people’s adherence to coronavirus-related behavior guidelines.
    Type of Medium: Online Resource
    ISSN: 1438-8871
    URL: Article
    DOI: 10.2196/19995
    Language: English
    Publisher: JMIR Publications Inc.
    Publication Date: 2020
    detail.hit.zdb_id: 2028830-X
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  • 10
    Online Resource
    Online Resource
    Oxidized Low‐Density Lipoprotein Inhibits THP‐1‐Derived Macrophage Autophagy via TET2 Down‐regulation
    Wiley ; 2015
    In:  Lipids Vol. 50, No. 2 ( 2015-02), p. 177-183
    Details
    In: Lipids, Wiley, Vol. 50, No. 2 ( 2015-02), p. 177-183
    Abstract: Oxidized low‐density lipoprotein (ox‐LDL) is an independent risk factor of atherosclerosis. However, the mechanism underlying its pro‐atherosclerosis roles has not yet been well explored. DNA demethylation modification, via DNA methyltransferases or ten‐eleven‐translocation (TET) family, is a crisis epigenetic regulation for various biological and pathological processes. This study aimed to investigate the effects of ox‐LDL on macrophage autophagy and its potential epigenetic mechanism. Results showed that after treatment with 0, 10, 20, 40 or 80 mg/L ox‐LDL for 24 h, the autophagy markers Beclin 1 and LC3 expression were obviously decreased at protein levels ( P 〈 0.05). The mRNA and protein expression of TET2 was evidently decreased ( P 〈 0.05). After pre‐treatment with TET2 siRNA, the mRNA and protein levels of Beclin 1 and LC3 decreased compared with the 80 mg/L treatment group ( P 〈 0.01). The mRNA and protein levels of Beclin 1 and LC3‐II were up‐regulated ( P 〈 0.05) in the 5‐aza‐2′‐deoxycytidine (a DNA methyltransferase inhibitor) of pretreatment group. Consistent with the Western blot results, cell immunofluorescence showed that the protein concentration of LC3‐II decreased in the TET2 siRNA group and increased in the 5‐aza‐2′‐deoxycytidine group. Taken together, these results showed that DNA demethylation modifications regulate ox‐LDL‐treated THP‐1 macrophages autophagy and TET2 might be a novel regulator.
    Type of Medium: Online Resource
    ISSN: 0024-4201 , 1558-9307
    URL: Article
    DOI: 10.1007/s11745-014-3977-5
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2030265-4
    SSG: 12
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