In:
Chemical Communications, Royal Society of Chemistry (RSC), Vol. 59, No. 8 ( 2023), p. 1006-1009
Abstract:
Metal-mediated nucleobase pairing can play a central role in the expression of metal-responsive DNA functions. We report the Cu II -mediated stabilisation of DNA duplexes bearing damaged nucleobases, 1, N 6 -ethenoadenine (εA), as metal-binding sites, which was utilised to construct a metal-responsive DNAzyme. Consecutive incorporation of three or more εA–εA mismatch pairs allowed for Cu II -dependent significant duplex stabilisation through metal-mediated εA–Cu II –εA base pairing. Subsequently, a split DNAzyme with three εA–Cu II –εA base pairs was strategically designed. The activity of the εA-modified DNAzyme was enhanced by 5.3-fold upon addition of Cu II ions. This study demonstrates the utility of εA lesions for building metal-responsive DNA architectures.
Type of Medium:
Online Resource
ISSN:
1359-7345
,
1364-548X
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2023
detail.hit.zdb_id:
1472881-3
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