In:
Helicobacter, Wiley, Vol. 19, No. 4 ( 2014-08), p. 260-271
Abstract:
Although the infection rate of H elicobacter suis is significantly lower than that of H elicobacter pylori , the H . suis infection is associated with a high rate of gastric mucosa‐associated lymphoid tissue ( MALT ) lymphoma. In addition, in vitro cultivation of H . suis remains difficult, and some H . suis ‐infected patients show negative results on the urea breath test ( UBT ). Materials and Methods Female C 57 BL /6J mice were orally inoculated with mouse gastric mucosal homogenates containing H . suis strains TKY or SNTW 101 isolated from a cynomolgus monkey or a patient suffering from nodular gastritis, respectively. The high‐purity chromosomal DNA samples of H . suis strains TKY and SNTW 101 were prepared from the infected mouse gastric mucosa. The SOL i D sequencing of two H . suis genomes enabled comparative genomics of 20 H elicobacter and 11 C ampylobacter strains for the identification of the H . suis ‐specific nucleotide sequences. Results Oral inoculation with mouse gastric mucosal homogenates containing H . suis strains TKY and SNTW 101 induced gastric MALT lymphoma and the formation of gastric lymphoid follicles, respectively, in C 57 BL /6 J mice. Two conserved nucleotide sequences among six H . suis strains were identified and were used to design diagnostic PCR primers for the detection of H . suis . Conclusions There was a strong association between the H . suis infection and gastric diseases in the C 57 BL /6 mouse model. PCR diagnosis using an H . suis ‐specific primer pair is a valuable method for detecting H . suis in gastric biopsy specimens.
Type of Medium:
Online Resource
ISSN:
1083-4389
,
1523-5378
DOI:
10.1111/hel.2014.19.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2020336-6
SSG:
12
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