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  • 1
    Online Resource
    Online Resource
    Evaluation of amniotic hydrogel’s impact on the development of murine pre-antral follicles in mouse model
    Viet Nam National University Ho Chi Minh City ; 2019
    In:  Science and Technology Development Journal - Natural Sciences Vol. 2, No. 4 ( 2019-08-13), p. 32-39
    Details
    In: Science and Technology Development Journal - Natural Sciences, Viet Nam National University Ho Chi Minh City, Vol. 2, No. 4 ( 2019-08-13), p. 32-39
    Abstract: Global average infertility rate is about 6– 12%, and in Vietnam at around 7.7%. As a result, there is a high demand for treatment, especially for female infertility. In vitro maturation (IVM) was evaluated and proven to be the most popular and promising at the moment. In long-term cultivation, the follicle was observed to extend, therefore, the usage of a supporting frame is quite necessary to maintain follicle’s natural sphere structure as well as completing the mature process. Amniotic membrane is an avascular membrane, composed of collagen, fibronectin, nidogen, proteoglycan, containing a big number of growth – factors with antimicrobial, anti-inflammatory, low immunogenicity and viscoelasticity properties. Amniotic hydrogel owns structure formed with thin fibers to help preserve the main component as collagen, which can turn to gel form at 37 degree Celsius. With those properties, amniotic hydrogel showed high potential as a scaffold for the follicle. When amniotic hydrogel is used as a scaffold for cultivating of secondary follicle (100 – 130 µm), the size of oocyte and follicle increased after 12 days of culturing, along with the formation of antrum. The results demonstrated the possibility to use amniotic hydrogel as a scaffold for the development of the secondary follicle.
    Type of Medium: Online Resource
    ISSN: 2588-106X , 2588-106X
    URL: Article
    DOI: 10.32508/stdjns.v2i4.807
    Language: Unknown
    Publisher: Viet Nam National University Ho Chi Minh City
    Publication Date: 2019
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  • 2
    Online Resource
    Online Resource
    Injectable Nanocurcumin-Formulated Chitosan-g-Pluronic Hydrogel Exhibiting a Great Potential for Burn Treatment
    Hindawi Limited ; 2018
    In:  Journal of Healthcare Engineering Vol. 2018 ( 2018), p. 1-14
    Details
    In: Journal of Healthcare Engineering, Hindawi Limited, Vol. 2018 ( 2018), p. 1-14
    Abstract: Burn wound healing is a complex multifactorial process that relies on coordinated signaling molecules to succeed. Curcumin is believed to be a potent antioxidant and anti-inflammatory agent; therefore, it can prevent the prolonged presence of oxygen free radicals which is a significant factor causing inhabitation of optimum healing process. This study describes an extension of study about the biofunctional nanocomposite hydrogel platform that was prepared by using curcumin and an amphiphilic chitosan-g-pluronic copolymer specialized in burn wound healing application. This formular (nCur-CP, nanocomposite hydrogel) was a free-flowing sol at ambient temperature and instantly converted into a nonflowing gel at body temperature. In addition, the storage study determined the great stability level of nCur-CP in long time using UV-Vis and DLS. Morphology and distribution of nCur in its nanocomposite hydrogels were observed by SEM and TEM, respectively. In vitro studies suggested that nCur-CP exhibited well fibroblast proliferation and ability in antimicrobacteria. Furthermore, second- and third-degree burn wound models were employed to evaluate the in vivo wound healing activity of the nCur-CP. In the second-degree wound model, the nanocomposite hydrogel group showed a higher regenerated collagen density and thicker epidermis layer formation. In third degree, the nCur-CP group also exhibited enhancement of wound closure. Besides, in both models, the nanocomposite material-treated groups showed higher collagen content, better granulation, and higher wound maturity. Histopathologic examination also implied that the nanocomposite hydrogel based on nanocurcumin and chitosan could enhance burn wound repair. In conclusion, the biocompatible and injectable nanocomposite scaffold might have great potential to apply for wound healing.
    Type of Medium: Online Resource
    ISSN: 2040-2295 , 2040-2309
    URL: Article
    DOI: 10.1155/2018/5754890
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2018
    detail.hit.zdb_id: 2545054-2
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  • 3
    Online Resource
    Online Resource
    Curcuminoid Co-Loading Platinum Heparin-Poloxamer P403 Nanogel Increasing Effectiveness in Antitumor Activity
    MDPI AG ; 2022
    In:  Gels Vol. 8, No. 1 ( 2022-01-14), p. 59-
    Details
    In: Gels, MDPI AG, Vol. 8, No. 1 ( 2022-01-14), p. 59-
    Abstract: Nanosized multi-drug delivery systems provide synergistic effects between drugs and bioactive compounds, resulting in increased overall efficiency and restricted side effects compared to conventional single-drug chemotherapy. In this study, we develop an amphiphilic heparin-poloxamer P403 (HP403) nanogel that could effectively co-load curcuminoid (Cur) and cisplatin hydrate (CisOH) (HP403@CisOH@Cur) via two loading mechanisms. The HP403 nanogels and HP403@CisOH@Cur nanogels were closely analyzed with 1H-NMR spectroscopy, FT-IR spectroscopy, TEM, and DLS, exhibiting high stability in spherical forms. In drug release profiles, accelerated behavior of Cur and CisOH at pH 5.5 compared with neutral pH was observed, suggesting effective delivery of the compounds in tumor sites. In vitro studies showed high antitumor activity of HP403@CisOH@Cur nanogels, while in vivo assays showed that the dual-drug platform prolonged the survival time of mice and prevented tail necrosis. In summary, HP403@CisOH@Cur offers an intriguing strategy to achieve the cisplatin and curcumin synergistic effect in a well-designed delivery platform that increases antitumor effectiveness and overcomes undesired consequences caused by cisplatin in breast cancer treatment.
    Type of Medium: Online Resource
    ISSN: 2310-2861
    URL: Article
    DOI: 10.3390/gels8010059
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2813982-3
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  • 4
    Online Resource
    Online Resource
    Effect of Blood Gel Derivatives on Wound Healing in Mouse Injured Tissue Models
    MDPI AG ; 2023
    In:  Gels Vol. 9, No. 10 ( 2023-09-28), p. 785-
    Details
    In: Gels, MDPI AG, Vol. 9, No. 10 ( 2023-09-28), p. 785-
    Abstract: Several previous studies in the field of assisted reproduction have focused on the use of blood gel derivatives, such as platelet-rich fibrin (PRF), as a treatment for endometrial rehabilitation. However, the ability to release growth factors and the gel form of this product led to the evolution of platelet lysates. In this study, blood gel derivatives, including PRF lysate, which was in liquid form, and PRF gel, were collected and evaluated for growth factors. It was shown to be effective in endometrial wound healing and regeneration in mouse injured uterine tissue models through structure and function (pinopode expression, embryo implantation) evaluation. The results demonstrated that the concentrations of growth factors, including PDGF-AB and VEGF-A, were higher in the PRF lysate compared to the PRF gel (p 〈 0.05). PRF lysate could release these growth factors for 8 days. Furthermore, both PRF gel and PRF lysate restored the morphology of injured endometrial tissues in terms of luminal and glandular epithelia, as well as uterine gland secretory activity. However, the presence of pinopodes and embryonic implantation were only observed in the PRF lysate group. It can be concluded that PRF lysate promotes wound healing in mouse injured tissue models in vitro, which can act as healing products in tissue repair.
    Type of Medium: Online Resource
    ISSN: 2310-2861
    URL: Article
    DOI: 10.3390/gels9100785
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2813982-3
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  • 5
    Online Resource
    Online Resource
    Thermosensitive nanocomposite hydrogel based pluronic-grafted gelatin and nanocurcumin for enhancing burn healing
    Viet Nam National University Ho Chi Minh City ; 2019
    In:  Science and Technology Development Journal - Natural Sciences Vol. 2, No. 4 ( 2019-08-14), p. 146-154
    Details
    In: Science and Technology Development Journal - Natural Sciences, Viet Nam National University Ho Chi Minh City, Vol. 2, No. 4 ( 2019-08-14), p. 146-154
    Abstract: Curcumin is extracted from turmeric exhibiting several biomedical activities. Unfortunately, less aqueous solubility was still a drawback to apply it in medicine. This study introduced a method to produce a thermosensitive nanocomposite hydrogel (nCur-PG) containing curcumin nanoparticles (nCur) which can overcome the poor dissolution of curcumin. Regarding to the method, a thermo-reversible pluronic F127-grafted gelatin (PG) play a role as surfactant to disperse and protect nanocurcumin from aggregation. The synthetic PG was identified by 1H-NMR. The obtained results via Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) indicated that the size of nCur was various in the range from 1.5 ± 0.5 to 128 ± 9.7 nm belong to amount of the fed curcurmin. The nCur-dispersed PG solution formed nCur-PG when the solution was warmed up to 34-35 oC. Release profile indicated sustainable release of curcumin from hydrogel. Thermosensitive nanocomposite hydrogel based pluronic-grafted gelatin and nanocurcumin performed potential application of the biomaterial in tissue regeneration.
    Type of Medium: Online Resource
    ISSN: 2588-106X , 2588-106X
    URL: Article
    DOI: 10.32508/stdjns.v2i4.822
    Language: Unknown
    Publisher: Viet Nam National University Ho Chi Minh City
    Publication Date: 2019
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  • 6
    Online Resource
    Online Resource
    Effects of advanced platelet‐rich fibrin combined with xenogenic bone on human periodontal ligament stem cells
    Wiley ; 2022
    In:  Clinical and Experimental Dental Research Vol. 8, No. 4 ( 2022-08), p. 875-882
    Details
    In: Clinical and Experimental Dental Research, Wiley, Vol. 8, No. 4 ( 2022-08), p. 875-882
    Abstract: In this study, we aimed to investigate the effects of a mixture of advanced platelet‐rich fibrin (A‐PRF) and xenogenic bone substitute material (XBSM) on the proliferation and migration of human periodontal ligament stem cells (hPDLSCs) based on the in vitro release of growth factors. Material and Methods The concentrations of platelet‐derived growth factor‐AB (PDGF‐AB) and vascular endothelial growth factor (VEGF) released by the A‐PRF‐XBSM mixture were estimated using enzyme‐linked immunoassay for up to 7 d. The A‐PRF‐XBSM mixture exudate was incubated with hPDLSCs. At Days 1, 3, 5, and 7, cell proliferation and migration were investigated by cell counting and wound‐healing assays. Results PDGD‐AB and VEGF were released from the A‐PRF‐XBSM mixture exudate for up to 7 days. hPDLSCs were cultured in media with various concentrations of the A‐PRF‐XBSM mixture exudate and exhibited their proliferation and migration ability. Furthermore, the factors released from the 100% A‐PRF‐XBSM mixture exudate had a substantial effect on cell migration, whereas those released from 4% and 20% A‐PRF‐XBSM mixture exudates stimulated hPDLSC proliferation. Conclusions A‐PRF‐XBSM mixture continuously released growth factors over 7 days and enhanced hPDLSC proliferation and migration. Therefore, A‐PRF in combination with XBSM might provide potential advantages for periodontal tissue regeneration.
    Type of Medium: Online Resource
    ISSN: 2057-4347 , 2057-4347
    URL: Issue
    URL: Article
    DOI: 10.1002/cre2.v8.4
    DOI: 10.1002/cre2.563
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2829558-4
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  • 7
    Online Resource
    Online Resource
    RESEARCH ON BIOCOMPATIBILITY OF CANCELLOUS - DERIVED ACELLULAR SCAFFOLD
    Viet Nam National University Ho Chi Minh City ; 2011
    In:  Science and Technology Development Journal Vol. 14, No. 4 ( 2011-12-30), p. 74-84
    Details
    In: Science and Technology Development Journal, Viet Nam National University Ho Chi Minh City, Vol. 14, No. 4 ( 2011-12-30), p. 74-84
    Abstract: Xenogeneic cancellous bone is a abundant resource on bone tissue engineering. But xenograft can arise a serious of immune responses. Thus, we decellularize xenogeneic cancellous bone for obtaining a great number of acellular and low antigenic scaffolds. Porcine cancellous bone (PCB) (4x4x3,5 cm3) were decellularized by acetone or chloroform/methanol (1:1). Biocompatibility of acellular scaffold was evaluated by seeding umbilical blood cells (UBCs) into scaffold and transplanting acellular scaffold to xeno-recipient. The result shows that PCB-derived scaffold treated in two decellularizing solutions have no cell. After 3 days, UBCs adhered on surface of scaffold and proliferated contiously after 12 days. After 8 days, scaffold makes a weak imflammatory response. The intial results show that PCB-derived acellular scaffold can apply in bone tissue engineering.
    Type of Medium: Online Resource
    ISSN: 1859-0128
    URL: Article
    DOI: 10.32508/stdj.v14i4.2038
    Language: Unknown
    Publisher: Viet Nam National University Ho Chi Minh City
    Publication Date: 2011
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  • 8
    Online Resource
    Online Resource
    Platelet-rich plasma as an ideal biomaterial for improving pregnancy of infertility mice
    Elsevier BV ; 2023
    In:  Journal of Science: Advanced Materials and Devices Vol. 8, No. 3 ( 2023-09), p. 100571-
    Details
    In: Journal of Science: Advanced Materials and Devices, Elsevier BV, Vol. 8, No. 3 ( 2023-09), p. 100571-
    Type of Medium: Online Resource
    ISSN: 2468-2179
    URL: Article
    DOI: 10.1016/j.jsamd.2023.100571
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2856527-7
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  • 9
    Online Resource
    Online Resource
    Experimental evidences for role of gel platelet-rich fibrin in treatment of mouse Asherman syndrome
    Open Science Publishers LLP ; 2023
    In:  Journal of Applied Biology & Biotechnology ( 2023)
    Details
    In: Journal of Applied Biology & Biotechnology, Open Science Publishers LLP, ( 2023)
    Type of Medium: Online Resource
    ISSN: 2455-7005 , 2347-212X
    URL: Article
    DOI: 10.7324/JABB.2023.11506
    Language: Unknown
    Publisher: Open Science Publishers LLP
    Publication Date: 2023
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  • 10
    Online Resource
    Online Resource
    Simplified conditions for storing and cryopreservation of dental pulp stem cells
    Elsevier BV ; 2017
    In:  Archives of Oral Biology Vol. 84 ( 2017-12), p. 74-81
    Details
    In: Archives of Oral Biology, Elsevier BV, Vol. 84 ( 2017-12), p. 74-81
    Type of Medium: Online Resource
    ISSN: 0003-9969
    URL: Article
    DOI: 10.1016/j.archoralbio.2017.09.002
    RVK:
    XA 29550
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2017
    detail.hit.zdb_id: 1496079-5
    SSG: 12
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