In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 11 ( 2023-11-30), p. e0295053-
Abstract:
The human malaria parasite Plasmodium falciparum is responsible for the majority of mortality and morbidity caused by malaria infection and differs from other human malaria species in the degree of accumulation of parasite-infected red blood cells in the microvasculature, known as cytoadherence or sequestration. In P . falciparum , cytoadherence is mediated by a protein called PfEMP1 which, due to its exposure to the host immune system, undergoes antigenic variation resulting in the expression of different PfEMP1 variants on the infected erythrocyte membrane. These PfEMP1s contain various combinations of adhesive domains, which allow for the differential engagement of a repertoire of endothelial receptors on the host microvasculature, with specific receptor usage associated with severe disease. We used a co-culture model of cytoadherence incubating human brain microvascular endothelial cells with erythrocytes infected with two parasite lines expressing different PfEMP1s that demonstrate different binding profiles to vascular endothelium. We determined the transcriptional profile of human brain microvascular endothelial cells (HBMEC) following different incubation periods with infected erythrocytes, identifying different transcriptional profiles of pathways previously found to be involved in the pathology of severe malaria, such as inflammation, apoptosis and barrier integrity, induced by the two PfEMP1 variants.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0295053
DOI:
10.1371/journal.pone.0295053.g001
DOI:
10.1371/journal.pone.0295053.g002
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10.1371/journal.pone.0295053.g003
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10.1371/journal.pone.0295053.g004
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10.1371/journal.pone.0295053.g005
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10.1371/journal.pone.0295053.g006
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10.1371/journal.pone.0295053.g007
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10.1371/journal.pone.0295053.g008
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10.1371/journal.pone.0295053.g009
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10.1371/journal.pone.0295053.g010
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10.1371/journal.pone.0295053.g011
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10.1371/journal.pone.0295053.t001
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10.1371/journal.pone.0295053.s001
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10.1371/journal.pone.0295053.s002
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10.1371/journal.pone.0295053.s003
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10.1371/journal.pone.0295053.s004
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10.1371/journal.pone.0295053.s005
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10.1371/journal.pone.0295053.s006
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10.1371/journal.pone.0295053.s007
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10.1371/journal.pone.0295053.s008
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10.1371/journal.pone.0295053.s009
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10.1371/journal.pone.0295053.s010
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10.1371/journal.pone.0295053.s011
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10.1371/journal.pone.0295053.s012
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10.1371/journal.pone.0295053.s013
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10.1371/journal.pone.0295053.s014
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10.1371/journal.pone.0295053.s015
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10.1371/journal.pone.0295053.s016
DOI:
10.1371/journal.pone.0295053.s017
DOI:
10.1371/journal.pone.0295053.s018
DOI:
10.1371/journal.pone.0295053.s019
DOI:
10.1371/journal.pone.0295053.s020
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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