In:
Arhiv za bioloske nauke, National Library of Serbia, Vol. 67, No. 1 ( 2015), p. 69-81
Abstract:
We examined the functions of the rat lymphocyte nuclear matrix after a single
exposure to total body irradiation with doses ranging from sublethal to
lethal. Irradiation induced systemic oxidative stress, detected as increased
activities of serum SOD and catalase, lymphocyte DNA damage, detected by the
Comet assay, and apoptosis. After irradiation with lower doses, the
recruitment of DNA repair centers on the matrix was observed by Western
analysis as increased levels of matrix-associated PARP-1, p53 and PCNA.
Augmented partitioning of the pro-survival transcription factor NF-?B on the
matrix was also detected after irradiation. Exposure to a lethal dose caused
breakdown of the matrix, observed as lamin B cleavage, and of the
matrix-associated DNA repair centers, detected as caspase-mediated PARP-1
proteolysis and loss of protein associations with the matrix. These findings
suggest that the nuclear matrix establishes functional 2 interactions in a
defensive mechanism, integrated in a decision-making process that resolves
cell fate.
Type of Medium:
Online Resource
ISSN:
0354-4664
,
1821-4339
DOI:
10.2298/ABS140124002M
Language:
English
Publisher:
National Library of Serbia
Publication Date:
2015
detail.hit.zdb_id:
2380842-1
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